Thickness of the basement membrane of bronchial epithelial cells in lung diseases as determined by transbronchial biopsy

Watanabe, Kentaro; Senju, Shoji; Toyoshima, Hideo; Yoshida, Minoru

doi:10.1016/s0954-6111(97)90254-7

Cited by 44 publications

(27 citation statements)

References 9 publications

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“…T1DM is associated with reduced lung capacities, elastic recoil, and dynamic lung compliance (55,57), likely a consequence of increased collagen cross-linking in the lung and thorax (9). Autopsy of diabetic lung tissue shows basement membrane thickening (32,73) including alveolar epithelium and capillary endothelium basal lamina (72,77). In streptozotocin-induced T1DM, the volume of alveolar wall is increased due to higher contents of collagen, elastin, and basal laminae (31,42,51), which partially result from slower degradation of connective tissue (43).…”

Section: Summary Of Findingsmentioning

confidence: 99%

Fatty diabetic lung: altered alveolar structure and surfactant protein expression

Foster

Ravikumar

Bellotto

et al. 2010

American Journal of Physiology-Lung Cellular and Molecular Phys

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nary dysfunction develops in type 2 diabetes mellitus (T2DM) in direct correlation with glycemia and is exacerbated by obesity; however, the associated structural derangement has not been quantified. We studied lungs from obese diabetic (fa/fa) male Zucker diabetic fatty (ZDF) rats at 4, 12, and 36 wk of age, before and after onset of T2DM, compared with lean nondiabetic (ϩ/ϩ) rats. Surfactant proteins A and C (SP-A and SP-C) immunoexpression in lung tissue was quantified at ages 14 and 18 wk, after the onset of T2DM. In fa/fa animals, lung volume was normal despite obesity. Numerous lipid droplets were visible within alveolar interstitium, lipofibroblasts, and macrophages, particularly in subpleural regions. Total triglyceride content was 136% higher. By 12 wk, septum volume was 21% higher, and alveolar duct volume was 36% lower. Capillary basement membrane was 29% thicker. Volume of lamellar bodies was 45% higher. By age 36 wk, volumes of interstitial collagen fibers, cells, and matrix were respectively 32, 25, and 80% higher, and capillary blood volume was 18% lower. ZDF rats exhibited a strain-specific increase in resistance of the air-blood diffusion barrier with age, which was exaggerated in fa/fa lungs compared with ϩ/ϩ lungs. In fa/fa lungs, SP-A and SP-C expression were elevated at age 14 -18 wk; the normal age-related increase in SP-A expression was accelerated, whereas SP-C expression declined with age. Thus lungs from obese T2DM animals develop many qualitatively similar changes as in type 1 diabetes mellitus but with extensive lipid deposition, altered alveolar type 2 cell ultrastructure, and surfactant protein expression patterns that suggest additive effects of hyperglycemia and lipotoxicity. diabetes mellitus; lung morphometry; lipid deposition; collagen; surfactant-associated proteins THE LUNG IS A RECOGNIZED TARGET of diabetic microangiopathy, manifested by modest restrictions of ventilatory capacity, lung volume, and diffusing capacity (12,24,41). Because alveolar microvascular reserves are extensive, pulmonary dysfunction is usually not the presenting complaint in diabetes mellitus, although modest pulmonary dysfunction may become overtly debilitating under physiological stress (e.g., high-altitude exposure or exercise) or following the loss of alveolar microvascular reserves brought on by aging or disease (23,24). In patients with type 1 diabetes mellitus (T1DM), we have found a significant restrictive defect associated with decreased lung diffusing capacity for carbon monoxide (DL CO ) at a given pulmonary blood flow, mainly due to a reduction of membrane conductance compared with age-matched nondiabetic healthy subjects (41, 52). Structural abnormalities observed at autopsy in diabetic human lungs include thickened epithelial and capillary basement membranes (72, 77), alveolar septal destruction, and enlarged air spaces (32). In streptozotocin-induced T1DM, volumes of basal laminae, extracellular matrix, and interstitial connective tissue are increased in diabetic lungs compared with contr...

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Section: Summary Of Findingsmentioning

confidence: 99%

Fatty diabetic lung: altered alveolar structure and surfactant protein expression

Foster

Ravikumar

Bellotto

et al. 2010

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…Remodelling of the airway wall has been assessed using bronchial biopsy specimens (3,(7)(8)(9)(10)(11)(12)(13) as well as inflammatory changes of airway using high resolution computed tomography (HRCT) (14)(15)(16)(17)(18)(19). The assesment using HRCT technique involved radiation risk and is not applicable for bronchial mural structure.…”

Section: Introductionmentioning

confidence: 99%

Airway Wall Structure Assessed by Endobronchial Ultrasonography and Bronchial Hyperresponsiveness in Patients With Asthma

Kita

Fujimura

Kurimoto

et al. 2010

Journal of Bronchology &Amp; Interventional Pulmonology

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“…Major alterations found in the airways of asthmatics are thickened basement membrane [18,19] and bronchial vessel dilation (reviewed by [20]). The authors found that there was a significant change in the thickness of the basement membrane after epithelial damage to the rat trachea that was due to the disruption of the TJ.…”

Section: Discussionmentioning

confidence: 99%

Effect of luminal osmolarity on ion content of connective tissue in rat trachea after epithelial damage

Relova¹,

Roomans²

2001

European Respiratory Journal

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Effect of luminal osmolarity on ion content of connective tissue in rat trachea after epithelial damage. A.J. Relova, G.M. Roomans. #ERS Journals Ltd 2001. ABSTRACT: The authors investigated the physical role of the airway epithelium in response to changes in the airway surface fluid9s composition by superperfusing the lumen with nonisotonic solutions. Morphological studies and measurements of changes in ion content in the underlying connective tissue (CT) were carried out.The study used an experimental model of isolated rat trachea. The trachea was mounted in an extraluminal organ bath with Ringer9s solution, whereas the lumen was perfused with a fluid in which the NaCl concentration was varied. The tissue was fixed for electron microscopy or frozen for X-ray microanalysis.X-ray microanalysis showed that the Na and Cl content of the CT increased with increasing luminal NaCl concentrations. This increase was significantly larger when the tight junctions had been damaged by exposure to ethylene glycol tetra-acetic acid. At high luminal NaCl concentrations, electron microscopy showed that a significant influx of fluid into the CT had occurred in tracheae with damaged epithelia. Damage to the epithelium also resulted in ultrastructural changes in myofibroblasts, increased diameter of capillaries, and thickening of the basement membrane.The epithelium evidently plays a crucial role in the regulation of the ion content of the connective tissue in the airway wall, and epithelial damage may explain the greater sensitivity to provocation with hypersomolar sodium chloride solutions or airway dehydration observed in patients with asthma or cystic fibrosis.

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Thickness of the basement membrane of bronchial epithelial cells in lung diseases as determined by transbronchial biopsy

Cited by 44 publications

References 9 publications

Fatty diabetic lung: altered alveolar structure and surfactant protein expression

Fatty diabetic lung: altered alveolar structure and surfactant protein expression

Airway Wall Structure Assessed by Endobronchial Ultrasonography and Bronchial Hyperresponsiveness in Patients With Asthma

Effect of luminal osmolarity on ion content of connective tissue in rat trachea after epithelial damage

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