Thidiazuron in Micropropagation of Aroid Plants

Chen, Jianjun; Wei, Xiangying

doi:10.1007/978-981-10-8004-3_4

Cited by 11 publications

(7 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thus, plantlets regenerated in this study were not via direct shoot organogenesis. There are at least two schools of thought regarding regeneration via PLBs: one considers that PLBs are somatic embryos [16,26], the other believes that regeneration through PLBs is an independent pathway differing from somatic embryogenesis and organogenesis [12,25,28], but it does have some components of both. A recent study showed that PLBs from Phalaenopsis aphrodite do not follow the somatic embryogenesis pattern [28].…”

Section: Discussionmentioning

confidence: 99%

“…In another non-orchid plant, nodal explants of S. podophyllum were cultured on MS in the presence of 2.26 µM 2,4-D, addition of 8.07 µM N-(2-chloro-4-pyridyl)-N -phenylurea (CPPU), 8.88 µM BA, 9.84 µM N-isopentenylaminopurine (2iP), and 9.08 µM TDZ induced 28.6%, 39.3%, 67.9%, and 71.9% nodal explants to produce PLBs, respectively [25]. TDZ is a synthetic phenyl urea derivative and has strong cytokinin-like activity exceeding most other commonly used adenine-type cytokinins in regulating plant morphogenesis [12]. Thus, TDZ in induction of PLBs in ginger family plants and monocot plants in general [25,33,36,53] deserves further investigation.…”

Section: Discussionmentioning

confidence: 99%

“…Micropropagation includes shoot culture and regeneration via organogenesis, somatic embryogenesis, and protocorm-like-bodies (PLBs) [11,12]. Shoot culture is the induction of axillary shoots from existing meristems, which is considered a reliable method for producing genetically identical plants without the occurrence of somaclonal variation [13,14].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Efficient Regeneration of Hedychium coronarium through Protocorm-Like Bodies

et al. 2020

Self Cite

View full text Add to dashboard Cite

Hedychium coronarium J. Koenig is a multipurpose plant with significant economic value, but it has been overexploited and listed as a vulnerable, near threatened or endangered species. In vitro culture methods have been used for propagating disease-free propagules for its conservation and production. However, explant contamination has been a bottleneck in in vitro propagation due to the use of rhizomes as the explant source. Plants in the family Zingiberaceae have pseudostems that support inflorescences, while rhizomes are considered true stems. The present study, for the first time, reported that the pseudostem bears nodes and vegetative buds and could actually be true stems. The evaluation of different sources of explants showed that mature node explants derived from the stem were the most suitable ones for in vitro culture because of the lowest contamination and the highest bud break rates. Culture of mature node explants on MS medium supplemented with 13.32, 17.76, and 22.20 μM 6-benzylaminopurine (BA), each in combination with 9.08 μM thidiazurin (TDZ) and 0.05 μM α-naphthaleneacetic acid (NAA) induced the conversion of buds to micro-rhizomes in six weeks. More than 96% of the micro-rhizomes cultured on MS medium supplemented with 17.76 μM BA, 6.81 μM TDZ, and 2.46 μM indole-3-butyric acid (IBA) were converted to globular-shaped clumps with protocorm-like bodies (PLBs). Further culture of a piece of the clumps induced more than 15 adventitious shoots. Adventitious roots were produced at the base of adventitious shoots, and plantlets were readily transplanted to a substrate for acclimatization in a shaded greenhouse. The survival rate of the plants in the greenhouse was up to 90%. Plants grew vigorously, and there were no off-types from the regenerated 11,100 plants. Our study also, for the first time, shows that H. coronarium can be regenerated via PLBs, which may represent a new way of the in vitro propagation of H. coronarium. The established protocol could be used for the increased propagation of H. coronarium for conservation or commercial production.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Efficient Regeneration of Hedychium coronarium through Protocorm-Like Bodies

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Most importantly, there is an increase of antioxidant enzymes ( Devireddy et al, 2021 ). Other outcomes include inducing shoot regeneration, roots and morphogenesis in Chinese water chestnut ( Wang et al, 2015 ), in vitro responses such as organogenesis ( Aremu et al, 2015 ; Werbrouck, 2016 ; Chen and Wei, 2018 ; Mazri et al, 2018 ), callus culture bioassays ( Kopecný et al, 2010 ), delayed senescence ( Nisler et al, 2016 ; Prernostava et al, 2018 ) and as basic as increasing yield of the cereal crops ( Ashikari et al, 2005 ; Nisler et al, 2021 ). Moreover, 44% increased zinc levels were present in the seeds of the transgenic barley plant along with drought tolerance.…”

Section: Biotechnological Responsesmentioning

confidence: 99%

Cytokinin Oxygenase/Dehydrogenase Inhibitors: An Emerging Tool in Stress Biotechnology Employed for Crop Improvement

Arora¹,

Sen²

2022

Front. Genet.

View full text Add to dashboard Cite

In order to meet the global challenges of food security, one of the foremost solutions lies in enhancing the crop productivity. This can be attained by considering key plant hormones such as cytokinins as agrochemicals as cytokinins in particular are known to control the essential processes of the plants. Even though, it has already been established since 1980s that the enzyme, cytokinin oxidase/dehydrogenase (CKO/CKX) deactivates cytokinins; the potential applications of manipulating these enzymes have mostly been speculated to have a high potential in the biotechnology industry and spreads to agriculture, horticulture and agroforestry. The enzyme is critical in maintaining a balanced level of cytokinins in plants. However, it is yet to be fully established that inhibiting this enzyme can be the constant source of improvement in the productivity of plants, even though success has been obtained in some economically important plant species. Furthermore, the impact efficiency of this enzyme may vary from plant to plant, which needs to be evaluated employing tissue culture and other extrinsic applications. This review intends to cover the relevant studies addressing any biological activity of this enzyme in the current context and any associated biotechnological applications specific to enhanced grain yield, abiotic stress tolerance, delayed senescence and in vitro organogenesis among various plants and not only cereals. Moreover, our study will identify the present gaps in research with respect to many important food crops, which will be useful for researchers who are actively involved in providing a foundation for a variety of genetically improved plants achieved through this manner. In addition to this, other ways of engineering the amount of cytokinin levels appropriate for signaling also needs to be analyzed in order to extend the benefits of cytokinin biology to other crops too. The application of these inhibitors can be considered among the best alternates as well as addition to genetically modified plants for overcoming the gaps in crop demand.

show abstract

“…Nur Fitriyah * Mawi Wahyudi 69 yang lebih tinggi dibandingkan dengan perlakuan lainnya. TDZ atau thidiazuron telah terbukti menjadi regulator pertumbuhan tanaman penting untuk mikropropagasi tanaman dan regenerasi tanaman melalui rute organogenesis tunas, embriogenesis somatik, dan tubuh seperti protokorm (Chen and Wei, 2018) selain itu kombinasi TDZ dengan hormon auksin juga mampu meningkatkan tinggi tanaman, dimana hormon auksin ini berfungsi untuk pemanjangan batang dan akar (Tambunan, dkk. 2018).…”

Section: Perlakuanunclassified

PENAMBAHAN ZAT PENGATUR TUMBUH PADA STEK MIKRO TANAMAN PORANG (Amorphophallus muelleri Blume) DAN TALAS BENENG (Xanthosoma undipes K.Koch)

Fitriyah¹,

Wahyudi²

2022

JIA

View full text Add to dashboard Cite

Tujuan dari penelitian ini untuk mengetahui efektivitas penambahan Zat Pengatur Tumbuh ( ZPT) pada pertumbuhan tunas dan perakaran stek mikro kulit umbi tanaman porang (Amorphophallus muelleri blume) dan talas beneng (Xanthosoma undipes k.koch). Rancangan Acak Lengkap (RAL) digunakan dalam penelitian ini dengan 8 perlakuan : Z1 = umbi porang tanpa ZPT, Z2= umbi porang + Auksin 1,5 ml/L, Z3= umbi porang +Thidiazuron 1 ppm, Z4= umbi porang +Auksin 1,5 ml/L + Thidiazuron 1 ppm, Z5 = umbi talas beneng tanpa ZPT, Z6= umbi talas beneng + Auksin 1,5 ml/L, Z7= umbi talas beneng +Thidiazuron 1 ppm, Z8= umbi talas beneng +Auksin 1,5 ml/L + Thidiazuron 1 ppm, masing-masing perlakuan di ulang 10 kali. Data dianalisa dengan menggunakan sidik ragam (ANOVA) pada taraf kepercayaan 95% dan apabila perlakuan yang memiliki sidik ragam yang nyata, dilanjutkan analisis lanjutan dengan menggunakan Uji BNT 5%. Penambahan zat pengatur tumbuh auksin, thidiazuron dan kombinasinya keduanya berpengaruh nyata terhadap persentase stek tumbuh, tinggi bibit, jumlah daun, panjang dan jumlah akar. Selain pengaruh dari ZPT eksogen, kemampuan tumbuh dalam perbanyakan vegetatif juga di pengaruh oleh jenis dan ukuran bahan tanam serta adanya titik tumbuh pada stek. Stek tanaman talas beneng (Xanthosoma undipes K.Koch) dari kulit umbi mempunyai kemampuan regenerasi yang lebih tinggi jika dibandingkan stek dari tanaman porang (Amorphophallus muelleri Blume).

show abstract

Thidiazuron in Micropropagation of Aroid Plants

Cited by 11 publications

References 82 publications

Efficient Regeneration of Hedychium coronarium through Protocorm-Like Bodies

Efficient Regeneration of Hedychium coronarium through Protocorm-Like Bodies

Cytokinin Oxygenase/Dehydrogenase Inhibitors: An Emerging Tool in Stress Biotechnology Employed for Crop Improvement

PENAMBAHAN ZAT PENGATUR TUMBUH PADA STEK MIKRO TANAMAN PORANG (Amorphophallus muelleri Blume) DAN TALAS BENENG (Xanthosoma undipes K.Koch)

Contact Info

Product

Resources

About