Thidiazuron-induced plant regeneration from hypocotyl cultures of St. John's wort ( Hypericum perforatum . cv 'Anthos')

Murch, Susan J.; Choffe, Kristen L.; Victor, J. M. R.; Slimmon, T.; KrishnaRaj, Sankaran; Saxena, Praveen K.

doi:10.1007/s002990050776

Cited by 87 publications

(52 citation statements)

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“…Whereas our results finding that, the maximum number of direct shootlets regeneration (24 shootlets /explant) was recorded with nodal explant cultured on MS supplemented with 0.1 NAA mg/l plus 1mg/l BA under light condition also that is in agreement with (Santarem and Astaria 2003), (Pasque et al, 2008) who observed organogenesis in calli tissue on medium supplemented with plant growth regulators such as BA, NAA and 2,4-D either alone or in combination. Direct organogenesis of H. perforatum using TDZ observed by Murch et al, (2000) this observation was differs slightly with our result whereas the results.…”

Section: Hypericin Analysiscontrasting

confidence: 99%

Hypericum sinaicum L. in Vitro Regeneration and Analysis of Hypericin Content

Khlifa¹,

Ibrahim²,

Bekhit³

et al. 2016

Int.J.Curr.Microbiol.App.Sci

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Section: Hypericin Analysiscontrasting

confidence: 99%

Hypericum sinaicum L. in Vitro Regeneration and Analysis of Hypericin Content

Khlifa¹,

Ibrahim²,

Bekhit³

et al. 2016

Int.J.Curr.Microbiol.App.Sci

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“…12 and 16 d and the shoots regenerated were deformed in morphology. Similar results were observed in Hypericum perforatum [15] Cassia angustifolia [24] and Rauvolfia serpentina [4]. To see the potential of the plants produced from the preculturing experiments and its reproducibility, shoots induced from 50 µM TDZ concentration and 8 d duration were again used for three extra rounds of propagation.…”

Section: Resultssupporting

confidence: 57%

Preculturing effect of thidiazuron onin vitroshoot multiplication and micropropagation round inCapparis decidua(Forsk.) an important multipurpose plant

Bukhari

Siddique

Perveen

2016

Acta Biologica Hungarica

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An efficient protocol was developed for clonal multiplication of an important shrub: Capparis decidua (Forsk.) Edgew, through in vitro shoot induction and multiplication from nodal explants. Pretreatment of nodal explants in a liquid Murashige and Skoog (MS) medium augmented with various thidiazuron (TDZ) concentrations at relatively high levels (5-100 µM) for different time duration (4, 8, 12 and 16 d), proved a significant approach for in vitro shoot production. After an initial exposure time to TDZ, nodal explants were inoculated onto a MS basal medium devoid of TDZ for further induction and proliferation. The highest regeneration rate (85%), average number of shoots/explant (8.7 ± 0.22) and maximum shoot length (3.9 ± 0.33 cm) were obtained from the nodal explants exposed to 50 µM TDZ for 8 d. The nodal explants excised from the proliferated cultures of TDZ (50 µM) for 8 d were used as explants and showed an enhancement rate after next three round of in vitro propagation. Best results for rooting was obtained by ex vitro treatment of shoots with 200 µM indole-3-butyric acid (IBA) for 20 min. as it produced an average of 5.7 ± 0.41 roots per microshoot with 4.4 ± 0.39 cm root length in 84% shoots. Different planting substrates was tested for maximum survival of hardening off micropropagated plantlets and soilrite proved most effective than others as 97.1 ± 7.21 plantlets survived. All micropropagated plants grew well in natural conditions and showed similar morphology to the mother plant.

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“…However, ensuring the safety, consistency and efficacy of whole plant preparations can be challenging. Products can be compromised by abiotic and biotic contaminants, misidentified species, unidentified phytochemicals, phytochemical variability between individuals within a species, degradation of medicinal metabolites during harvest, processing and handling, as well as a general lack of knowledge about the unique physiology of medicinal plants (Murch et al 2000a). To address these issues and to ensure the safety, quality and efficacy of products, the Government of Canada created the Natural Health Products Directorate (NHPD) within Health Canada, and enacted the new legislation (JUS-601727) to govern the manufacture and marketing of NHPs.…”

Section: Mots Clésmentioning

confidence: 99%

“…PLANT PRODUCTION Since the therapeutic properties of medicinal plants may not be attributed to single compounds, but rather require whole plant preparations (Murch et al 2000a;Roscetti et al 2004), the production of chemically consistent crops is essential. Concerns such as crop quality, variability, levels of active constituents, adulteration and contamination with other misidentified species, bacteria, and fungi (Murch et al 2000a) may be addressed by combining in vitro propagation and controlled environment greenhouse technologies.…”

Section: Strategies For Medicinalmentioning

confidence: 99%

“…There are numerous factors that contribute to the chemical inconsistency, such as complex genetics related to secondary metabolite biosynthesis, adulterations with misidentified plant species, plant-environment interactions, and herbivory (Murch et al 2000a). Genetic infidelity is a major cause of variability in St. John's wort as the species reproduces in at least 11 different ways.…”

Section: St John's Wort: Chemical Profiles In Thementioning

confidence: 99%

See 1 more Smart Citation

St. John’s wort (Hypericum perforatum L.): Challenges and strategies for production of chemically-consistent plants

Murch¹,

Saxena²

2006

Can. J. Plant Sci.

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Murch, S. J. and Saxena, P. K. 2006. St. John's wort (Hypericum perforatum L.): Challenges and strategies for production of chemically consistent plants. . Plants are by far the most important source of natural therapeutics, and the role of plants in enhancing the longevity and the quality of life is increasingly accepted throughout the world. A series of problems with medicinal plant products, such as contamination with biological and environmental pollutants, quantitative and qualitative variations of bioactive compounds, adulteration with misidentified species, and the concern of unsustainable harvest, has prompted the introduction of regulations to ensure the quality and safety of medicinal plant products in Canada. In the future, Natural Health Products in Canada will be manufactured to a new standard of quality and these changes in the industry have necessitated new approaches to the breeding, production, and processing of medicinal plant tissues. The continuing growth in the medicinal plant marketplace has also brought about the challenge of maintaining a balance between consumer demand and the need to protect medicinal biodiversity. St. John's wort (Hypericum perforatum L.) is one of the most popular medicinal plants with a history of use spanning more than two millennia and modern studies demonstrating efficacy. However, inconsistencies in the results of various clinical trials and difficulties in identifying a specific medicinal molecule with defined pharmaceutical function prompted our efforts to improve St. John's wort products. Development of elite varieties with predictable phytochemical profiles, mass clonal propagation in vitro, large-scale production in sterile environments and controlled environment production systems, have been combined to produce a new standard in the production of St. John's wort. . Les plantes restent de loin la principale source de substances médicinales naturelles et, partout dans le monde, on reconnaît de plus en plus leur rôle dans un accroissement de la longévité et l'amélioration de la qualité de vie. Diverses difficultés (contamination des substances médicinales végé-tales par des polluants organiques et environnementaux, variation de la quantité et de la qualité des composés bioactifs, adultération avec des espèces non identifiées, récolte à des taux insoutenables) ont incité les administrations publiques du Canada à réglementer ces plantes afin d'en garantir la qualité et l'innocuité. Dorénavant, au Canada, les produits de santé naturels seront préparés en fonction d'une nouvelle norme qualitative et ce changement a contraint l'industrie à adopter de nouvelles approches à l'hybridation, à la culture et à la transformation des plantes médicinales. Parallèlement, le marché poursuit son essor, si bien qu'il est difficile de répondre à la demande tout en préservant la biodiversité des plantes. Le millepertuis (Hypericum perforatum L.) figure parmi les plus populaires. On utilise cette plante depuis plus de deux millénaires et des études contemporaines en ont prouvé l'e...

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Thidiazuron-induced plant regeneration from hypocotyl cultures of St. John's wort ( Hypericum perforatum . cv 'Anthos')

Cited by 87 publications

References 0 publications

Hypericum sinaicum L. in Vitro Regeneration and Analysis of Hypericin Content

Hypericum sinaicum L. in Vitro Regeneration and Analysis of Hypericin Content

Preculturing effect of thidiazuron onin vitroshoot multiplication and micropropagation round inCapparis decidua(Forsk.) an important multipurpose plant

St. John’s wort (Hypericum perforatum L.): Challenges and strategies for production of chemically-consistent plants

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