Thidiazuron-Induced Protocorm-Like Bodies in Orchid: Progress and Prospects

Kundu, Suprabuddha; Gantait, Saikat

doi:10.1007/978-981-10-8004-3_13

Cited by 17 publications

(6 citation statements)

References 58 publications

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“…This might be because P. SCBG Huihuang90 is sensitive to exogenous plant growth regulators. TDZ is highly effective at very low concentrations [ 11 ] and so is not applicable to P. SCBG Huihuang90. Low concentrations of 2,4-D promote the proliferation of PLB masses.…”

Section: Discussionmentioning

confidence: 99%

“…In orchids, the formation of PLB is regulated by various factors, and plant growth regulators (PGRs) are one of the most important factors [ 8 ]. Thidiazuron (TDZ), 2,4-D, or their combination were considered to be the most suitable PRGs for callus and PLB induction [ 8 , 11 ]. The induction of PLB formation by 2,4-D or TDZ has been confirmed to be successful in some orchids such as Cymbidium [ 12 ], Phalaenopsis [ 13 ], and Dendrobium [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

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Tissue Culture via Protocorm-like Bodies in an Orchids Hybrids Paphiopedilum SCBG Huihuang90

Guo,

Chen,

Yin

et al. 2024

Plants

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This study successfully established an efficient in vitro propagation system for Paphiopedilum SCBG Huihuang90 via protocorm-like body (PLB) formation from seed-derived calluses, PLB proliferation and differentiation, root induction and greenhouse acclimatization. Furthermore, 1/2 Murashige and Skoog (MS) + 0.025 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D) was suitable for the proliferation of PLBs, and 1/2MS + 10% coconut water (CW, v/v) + 0.5 g/L activated carbon (AC) was suitable for PLB differentiation. PLBs at different developmental stages required different kinds of sugars. This study provided a reference for research on the propagation techniques of other Paphiopedilum.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tissue Culture via Protocorm-like Bodies in an Orchids Hybrids Paphiopedilum SCBG Huihuang90

Guo,

Chen,

Yin

et al. 2024

Plants

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“…Among the PGRs used, thidiazuron (TDZ) is considered the most powerful synthetic PGR for in vitro reprogramming cell developmental fate initiating a wide variety of morphogenic responses [8,9]. It was noted that TDZ-induced in vitro morphogenesis from Rhododendron leaf explants could occur through direct [10,11], indirect organogenesis [12,13] as well as somatic embryogenesis [14].…”

Section: Introductionmentioning

confidence: 99%

Direct Regeneration and Morpho-Histological Study of de novo Shoot Development from Leaf Explants of Rhododendron mucronulatum Turcz.

Zaytseva

2024

Russ J Plant Physiol

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“…In previous work, the stationary microshoot cultures of R. mucronulatum based on TDZ treatment as a trigger of shoot morphogenesis have been established (Novikova et al 2020). TDZ, phenyl urea, is the most suitable plant growth regulator (PGR) for rapid and effective in vitro propagation (Kundu and Gantait 2018;Novikova and Zaytseva 2018). Moreover, TDZ was shown to affect not only endogenous cytokinin and auxin production but the level of secondary metabolites and essential oils in many medicinally important plants (Liu et al 2007;Wannakrairoj and Tefera 2012;Ali et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Thidiazuron and LED Lighting Enhance Taxifolin and Rutin Production in Rhododendron mucronulatum Turcz. Microshoot Culture

Petruk

Novikova

2022

J Plant Growth Regul

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Rhododendron mucronulatum Turcz., distributed throughout the northern region of East Asia has been considered to be an alternative natural source of taxifolin (dihydroquercetin) and rutin. The present study was conducted based on a biotechnological approach to develop an environment friendly and efficient system to produce taxifolin and rutin in R. mucronulatum microshoots, using different thidiazuron (TDZ) treatments (0.1; 0.5; 2.5 µM) in combination with various types of lighting including fluorescent (FL) and light-emitting diode (LED) (R/B− 80% red + 20% blue; 5LED-20% red + 20% blue + 20% green + 20% yellow + 20% white). The highest number of shoots per explant was obtained under 0.5 µM TDZ combined with 5LED in comparison with FL lighting. Among shoot clusters obtained under different lighting types and TDZ concentrations, a considerable increase in fresh and dry weight was observed in ones cultivated on medium, supplemented with 2.5 µM TDZ under FL and 0.5 µM TDZ at R/B or 5LED. The content of total chlorophylls in R. mucronulatum microshoots increased on TDZ-free medium under FL lighting, whereas, the TDZ treatment decreased chlorophylls concentration at FL and 5LED. The use of 0.1 µM TDZ at 5LED decreased the ratio of chlorophylls a + b to carotenoids and led to the highest accumulation of taxifolin and rutin, quercetin, hyperoside, and avicularin. Thus, it has been demonstrated that the application of combined action of LED and TDZ has great potential in terms of propagation efficiency, biomass accumulation, and taxifolin and rutin production in R. mucronulatum microshoots.

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Thidiazuron-Induced Protocorm-Like Bodies in Orchid: Progress and Prospects

Cited by 17 publications

References 58 publications

Tissue Culture via Protocorm-like Bodies in an Orchids Hybrids Paphiopedilum SCBG Huihuang90

Tissue Culture via Protocorm-like Bodies in an Orchids Hybrids Paphiopedilum SCBG Huihuang90

Direct Regeneration and Morpho-Histological Study of de novo Shoot Development from Leaf Explants of Rhododendron mucronulatum Turcz.

Thidiazuron and LED Lighting Enhance Taxifolin and Rutin Production in Rhododendron mucronulatum Turcz. Microshoot Culture

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