Thidiazuron‐Induced Tissue Culture Regeneration from Quartered‐Seed Explants of Arachis Paraguariensis

Abstract

Arachis paraguariensis Chodat & Hassl. is a potential source of novel genes for the genetic improvement of cultivated peanut (Arachis hypogaea L.) because some of its accessions show high levels of resistance to early leaf spot caused by Cercospora arachidicola Hori. In this study, induction of high frequency shoot regeneration from quartered‐seed explants was accomplished for six plant introductions of A. paraguariensis under continuous light on Murashige and Skoog (MS) medium containing 4.4 mg L−1 thidiazuron (TDZ) in combination with 2.2 mg L−1 6‐γ‐γ‐(dimethylallylamino)‐purine (2ip). Recovery of a moderately high number of plantlets per quarter seed cultured was also achieved on medium containing 4.4 mg L−1 thidiazuron in combination with 1.1 to 4.4 mg L−1 6‐benzylaminopurine (BAP) with bud formation occurring as early as 1 wk after culture initiation. There were no differences in seed production or in early leaf spot incidence between plants of two genotypes of A. paraguariensis derived from seeds vs. in vitro tissue culture derived plants; however, cultivated peanut cv. Florunner had a higher incidence of early leaf spot.

EMBRIOGÉNESIS SOMÁTICA INDUCIDA CON ESTRÉS OSMÓTICO EN Bouteloua curtipendula (Michx.) Torr., ESPECIE RECALCITRANTE Y APOMÍCTICA

“…Frecuentemente, los factores genotipo, medio de cultivo, fuente de auxinas, concentración (Rakshit et al, 2010), tipo de explante y edad (Aina et al, 2012), son considerados en la inducción de callos morfogénicos para incrementar la respuesta de regeneración de plantas morfológicamente normales. Se ha reportado alta eficiencia en la inducción de callos en diferentes explantes en especies apomícticas, pero la capacidad de regeneración ha sido muy pobre (Hu et al, 2006;Bernal-Flores et al, 2013), capacidad que constituye la principal limitante para la obtención de protocolos eficientes de mejoramiento.…”