Thin Cell Layer Studies of Vegetable, Leguminous and Medicinal Plants

Nhựt, Dương Tấn; Silva, Jaime A. Teixeira da; Lệ, Bùi Văn; Van, K. Tran Thanh

doi:10.1007/978-94-017-3522-3_10

Cited by 11 publications

(10 citation statements)

References 127 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This technique promoted rapid and intensive shoot regeneration with shoot buds developing within 7 days for the most reactive genotype. These results compare favourably with recent studies of shoot regeneration of rapeseed from traditional explants (Tang et al 2003;Akasaka-Kennedy et al 2005) and from TCLs (Klimaszewska and Keller 1985;Nhut et al 2003). Based on these two last references, using TCL explants provides a method for efficient bud regeneration but the explants were excised longitudinally and originated from 6-weekold flowering plants cultured in greenhouse.…”

Section: Discussionsupporting

confidence: 78%

“…For B. napus, it is known that shoot regeneration largely depends on genotype, explant source (Akasaka-Kennedy et al 2005), PGR concentration and sucrose content (Nhut et al 2003;Tang et al 2003). Previous studies showed that organogenesis could be achieved from various explants: cotyledons (Narasimhulu and Chopra 1988), immature cotyledons (Turgut et al 1998), hypocotyls (Khehra and Mathias 1992), stem sections (O'Neill et al 1996) and longitudinal Thin Cell Layers (lTCLs) from stems (Klimaszewska and Keller 1985;Nhut et al 2003). However, to the best of our knowledge, there is no report on plant regeneration system using tTCLs excised from hypocotyls or petioles of young plants cultivated in vitro.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Rapid shoot regeneration from thin cell layer explants excised from petioles and hypocotyls in four cultivars of Brassica napus L.

Ghnaya

Charles

Branchard

2007

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

The present work describes a procedure that allows for the easy and rapid induction of caulogenesis in four cultivars of Brassica napus L. from transversal Thin Cell Layers (tTCLs). In order to investigate the regeneration ability of this crop, the effects of genotype, explant source and culture medium were examined on shoot regeneration. The tTCL explants were excised from hypocotyl and petiole of 2-week-old seedlings and cultured on a solid basal MS medium supplemented with a-naphthaleneacetic acid (NAA: 0.1-0.4 mg l -1 ), 6-benzylamino-purine (BAP: 1-4 mg l -1 ) and sucrose (20-40 g l -1 ). A significant genotypic effect was observed between the four cvs; Jumbo and Drakkar displayed higher capacities to produce shoots than Pactol and Cossair. Regeneration commenced earlier and the percentage of shoot-producing explants as well as the number of shoots per regenerating explant was greater. The comparison between the regeneration ability of different explants showed that the hypocotyls exhibited a high rate of shoot organogenesis when they were cultured on MS medium supplemented with 3 mg l -1 BAP, 0.3 mg l -1 NAA and 30 g l -1 sucrose. Adventitious shoot buds developed from 46% of the tTCLs, with a mean of 7.5 buds. Furthermore, the method was fast with shoot formation occurring by 7 days culture. Plantlets regenerated from all shoots and developed normally. The regenerated plants were fertile and identical to source plants.

show abstract

Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Rapid shoot regeneration from thin cell layer explants excised from petioles and hypocotyls in four cultivars of Brassica napus L.

Ghnaya

Charles

Branchard

2007

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…TCL technology has also been effectively used in the micropropagation of vegetable, leguminous, and medicinal plants, including Amaranthus edulis (amaranth), Beta vulgaris (sugar beet), B. napus (oilseed rape), Lupinus spp. (lupin), Panax ginseng (ginseng), and Phaseolus vulgaris (common bean) (Nhut et al 2003). Influence of orientation of explants on shoot multiplication Efficiency of shoot regeneration was also affected by the orientation of tTCL nodal segments.…”

Section: Resultsmentioning

confidence: 99%

An improved micropropagation of Spilanthes acmella L. through transverse thin cell layer culture

et al. 2009

View full text Add to dashboard Cite

An efficient and improved in vitro propagation system for Spilanthes acmella L. using transverse thin cell layer (tTCL) culture system was established. The frequency of shoot regeneration from tTCL nodal segments was affected by concentrations of plant growth regulators and orientation of the explant. MS (Murashige and Skoog in Physiol Plant 15:473-497, 1962) medium with 5.0 mg dm -3 BAP was optimal for shoot regeneration. Upon this medium, the explant inoculated in the upright orientation exhibited a high frequency of shoot regeneration (about 97%), and the highest number of shoots (31.5) per explant. The intact node (1.0-1.5 cm) cultured on the same medium had significantly lower shoot multiplication ability with only 4.5 shoots per responsive explant. As compared to BAP alone, the combination of BAP and Kin or NAA did not have positive effects on shoot multiplication from tTCL nodal segments. Rooting of shoots was achieved on growth regulator free full-strength MS medium. Plantlets were transplanted into soil with 90-100% survival rate.

show abstract

“…TCL technology originated almost 30 years ago with the controlled development of various organs on tobacco pedicel (Tran Thanh Van, 1973 (Nhut et al, 2003b); cereals and grasses, including Digitaria sanguinalis (large crabgrass), Oryza sativa (rice), Sorghum bicolor (sorghum), and Zea mays (corn) (Nhut et al, 2003a); fruits, including Musa sp. (banana), Citrus spp.…”

Section: Callus Inductionmentioning

confidence: 99%

Somatic embryogenesis from leaf transverse thin cell layer derived-callus of Vietnamese ginseng (Panax vietnamensis Ha et Grushv.)

Hiền¹,

Huy²,

Chien³

et al. 2016

Vietnam J. Biotechnol.

Self Cite

View full text Add to dashboard Cite

SUMMARYNo report on plant regeneration via somatic embryogenesis of P. vietnamensis has been previously published. In the present study, somatic embryogenesis via callus formation from cultures of leaf transverse thin cell layers (tTCLs) of Vietnamese ginseng (Panax vietnamensis Ha et Grushv.) was investigated. α-naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BA) and thidiazuron (TDZ) were added separately and in combination into the culture media. Explant necrosis or low callogenesis rates were observed when 1-mm wide leaf tTCLs were cultured on media with TDZ, BA, 2,4-D or NAA. On the other hand, calli were successfully induced from the tTCL explants cultured on medium supplemented with either 2,4-D and BA or 2,4-D and TDZ. Callogenesis was observed under both light and dark conditions. The highest callogenesis rate

show abstract

Thin Cell Layer Studies of Vegetable, Leguminous and Medicinal Plants

Cited by 11 publications

References 127 publications

Rapid shoot regeneration from thin cell layer explants excised from petioles and hypocotyls in four cultivars of Brassica napus L.

Rapid shoot regeneration from thin cell layer explants excised from petioles and hypocotyls in four cultivars of Brassica napus L.

An improved micropropagation of Spilanthes acmella L. through transverse thin cell layer culture

Somatic embryogenesis from leaf transverse thin cell layer derived-callus of Vietnamese ginseng (Panax vietnamensis Ha et Grushv.)

Contact Info

Product

Resources

About