Thin Cell Layer Technology in Micropropagation of Jatropha curcas L.

Nhựt, Dương Tấn; Giap, Do Dang; Loan, Nguyen Thi Kim; Huy, Nguyễn Xuân; Hải, Nguyễn Thanh; Chien, Hoang Xuan; Tuan, Tran Trong; Du, Thai Xuan

doi:10.1007/978-94-007-5500-0_2

Cited by 5 publications

(5 citation statements)

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“…In order to overcome the recalcitrance, characteristic of adult plants of J. curcas for micropropagation (Jyoti et al, 1998;Nhut et al, 2013), it was sought to develop a protocol to induce SE using different types of explants from adult mother plants. Several types of explants were tested, and it was found that tTCLs of young petioles were the most suitable explants (Figure 2B).…”

Section: Resultsmentioning

confidence: 99%

“…It could be observed that callus started developing on the central part of the petiole TCL and continued all over the explant (Figure 2C). Moreover, Nhut et al (2013) described a protocol for propagating J. curcas through direct and indirect SE and Organogenesis using tTCL. However, they also performed it on very young plants and used leaves lamina, while in this study, adult plants were used given the advantage that the protocol can be used for elite genotypes.…”

Section: Resultsmentioning

confidence: 99%

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A novel genotype-independent technique for successful induction of somatic embryogenesis of adult plants of Jatropha curcas L. using petiole transverse Thin Cell Layer (TCL)

Mendoza-PeÃ±a¹,

Hvoslef-Eide²

2021

Afr. J. Biotechnol.

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Developing a protocol for somatic embryogenesis (SE) for adult mother plants of Jatropha curcas, aids to overcome problems such as harvesting time and an uneven yield, providing the opportunity to propagate proven elite genotypes. Until now, several authors have achieved SE in J. curcas using different explants, however, in none of these protocols adult plants have been used as mother plant material. Furthermore, the challenge of overcoming the morphogenesis limitations successfully and further regeneration of adult plants of J. curcas has been great. The main objective of this study was to develop a somatic embryogenesis protocol for adult plants of the oil producing J. curcas. transverse thin cell layers (tTCL) of young petioles were used as explants. Half strength Y3 and MS media were used containing three different concentrations of 2,4-dichloropherioxyacetic acid and 6-benzilaminopurine in a factorial trial. Nine accessions of J. curcas of various ages (3-12 years.) were tested using this protocol. All accessions responded positively to callus induction and to the induction of somatic embryos, demonstrating the protocol to be genotype-independent. This study enables micropropagation of adult of proven elite plants of J. curcas via somatic embryogenesis.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

A novel genotype-independent technique for successful induction of somatic embryogenesis of adult plants of Jatropha curcas L. using petiole transverse Thin Cell Layer (TCL)

Mendoza-PeÃ±a¹,

Hvoslef-Eide²

2021

Afr. J. Biotechnol.

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“…O 2,4-D, considerado como a principal auxina de indução da embriogênese somática, também pode ser requerida em altas concentrações e/ou por longos períodos para iniciar a sequência de eventos que desencadeiam a embriogênese somática (FEHER et al, 2003). Em pinhão-manso, Nhut et al (2013) também obtiveram sucesso na indução de calos a partir de folhas utilizando o 2,4-D.…”

Section: Caracterização Morfológica E Anatômica De Calos Formados Em Hipocótilos E Folhas Cotiledonares De Pinhão-mansounclassified

“…curcas têm sido relatados por autores (WEIDA et al 2003;JHA et al 2007;NHUT et al 2013;SHUKLA et al 2013;SIANG et al 2014;CHIANGMAI et al 2015;YING et al 2015). Entretanto, apesar dos vários trabalhos encontrados na literatura, ainda não existe um protocolo confiável para a propagação in vitro da espécie, sendo observado geralmente trabalhos não reproduzíveis.…”

Section: Introductionunclassified

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