Thin-film conductometric biosensors for glucose and urea determination

Шульга, А.А.; Солдаткин, А. П.; El'skaya, A. V.; Dzyadevich, S. V.; Patskovsky, Sergiy; Strikha, V. I.

doi:10.1016/0956-5663(94)80124-x

Cited by 85 publications

(21 citation statements)

References 10 publications

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“…This change can be simulated by the change of background conductivity which in our experiments was realized by varying the KCl concentration in solution and its temperature [11, 13, 14, 16, 17]. The change in solution background conductivity has been shown to affect mainly the high-frequency part of admittance.…”

Section: Transducers For Conductometric Biosensorsmentioning

confidence: 99%

“…Various materials have been tested: platinum [5, 15-22], gold [5, 11, 14, 18, 23-25], aluminium [11, 14, 26], nickel [11, 14, 26], copper [11, 14, 21], titanium [5, 11, 14], chromium [11, 14], Ta 2 O 5 [12, 13], silver [22] and carbon [27]. In general, all these materials are suitable, especially when high-frequency ac is used.…”

Section: Transducers For Conductometric Biosensorsmentioning

confidence: 99%

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Conductometric Microbiosensors for Environmental Monitoring

Jaffrézic‐Renault

Dzyadevych

2008

Sensors

218

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This review presents the principles of conductometric measurements in ionic media and the equivalent electrical circuits of different designs for conductometric measurements. These types of measurements were first applied for monitoring biocatalytic reactions. The use of conductometric microtransducers is then presented and detailed in the case of pollutant detection for environmental monitoring. Conductometric biosensors have advantages over other types of transducers: they can be produced through inexpensive thin- film standard technology, no reference electrode is needed and differential mode measurements allow cancellation of a lot of interferences. The specifications obtained for the detection of different pesticides, herbicides and heavy metal ions, based on enzyme inhibition, are presented as well as those obtained for the detection of formaldehyde, 4- chlorophenol, nitrate and proteins as markers of dissolved organic carbon based on enzymatic microbiosensors.

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Section: Transducers For Conductometric Biosensorsmentioning

confidence: 99%

Section: Transducers For Conductometric Biosensorsmentioning

confidence: 99%

Conductometric Microbiosensors for Environmental Monitoring

Jaffrézic‐Renault

Dzyadevych

2008

Sensors

218

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“…According to previous conductometric studies (Sheppard and Mears, 1996;Shulga et al, 1994;Watson et al, 1987Watson et al, -1988 urease enzyme was selected for the microbiosensor elaboration due to its high stability and activity that leads to the production of ammonium, bicarbonate and hydroxide ions through the hydrolysis of urea.…”

Section: Introductionmentioning

confidence: 99%

Urease–gelatin interdigitated microelectrodes for the conductometric determination of protease activity

Ionescu

Fillit

Jaffrézic‐Renault

et al. 2008

Biosensors and Bioelectronics

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“…Glucose oxidase (GOD) from Aspergillus niger was crosslinked with bovine serum albumin following the methods outlined by Shul'ga and co-workers [15,16]. A quantity of 1 mL of 5 % w/v GOD preparation was placed on the surface of the electrode and spread into a thin layer.…”

Section: Enzyme Preparation and Immobilizationmentioning

confidence: 99%

Frequency Domain Selection of the Peroxide Signal for Amperometric Biosensors

et al. 1998

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The amperometric biosensor has become a convenient analytical device concept since the biorecognition element can be highly specific to the analyte of interest when coupled to the appropriate transducer. However, a shortcoming of this system is that the transducer alone is not necessarily specific to the analyte, but only in conjunction with the recognition element. Since many applications require that the sensor be used in complex analytical media, other sample species may cause a direct response at the transducer, for example electroactive species may be directly oxidized or reduced at an electrode in an electrochemical sensor, yielding an erroneous signal. Filtration/membrane barriers have been proposed to overcome this, but this also eliminates any information about the interferent. In this article the role of impedence spectroscopy is examined as a technique to probe overlapping electrochemical signals by separation in the frequency domain. Hydrogen peroxide is examined as a model to test the feasibility of extraction of quantitative data in this control mode, and experimental conditions are determined where analytical data may be obtained to extract the peroxide signal from other electrochemical information in an enzyme biosensor based on peroxide determination.

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Thin-film conductometric biosensors for glucose and urea determination

Cited by 85 publications

References 10 publications

Conductometric Microbiosensors for Environmental Monitoring

Conductometric Microbiosensors for Environmental Monitoring

Urease–gelatin interdigitated microelectrodes for the conductometric determination of protease activity

Frequency Domain Selection of the Peroxide Signal for Amperometric Biosensors

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