Thin fluorinated polymer film microcavity arrays for 3D cell culture and label-free automated feature extraction

Abstract

A microengineered fluorinated ethylene-propylene based cell culture system was developed for automated, label-free feature extraction from 3D stem cell cultures.

Direct deep UV lithography to micropattern PMMA for stem cell culture

“…These thin films enabled the use of Substrate Modification And Replication by Thermoforming (SMART) technology, which has been previously described [ 3 , 13 , [39] , [40] , [41] ]. The method utilizes microthermoforming [ 42 , 43 ] in combination with pre-processed polymer films to generate patterned, curvilinear surfaces. For this purpose, we fabricated a brass mold with microcavity arrays of 800 μm diameter ( Fig.…”