Thin Frozen Film Method for Visualization of Storage Proteins in Mature Rice Grains

Saito, Yuhi; Nakatsuka, Nobuaki; Shigemitsu, Takanari; Tanaka, Kunisuke; Morita, Shigeto; Satoh, Shigeru; Masumura, Takehiro

doi:10.1271/bbb.80337

Cited by 17 publications

(18 citation statements)

References 9 publications

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“…Distribution of 13 kDa prolamin and 23 kDa glutelin in rice grains of seed-protein mutant cultivars was visualized in this study using immunofluorescence microscopic analysis according to the procedures used by Saito et al (2008). Previously, the distribution of each protein in rice grains of seed-protein mutant cultivars had been investigated by SDS-PAGE using rice grains milled at different percentages (Ohdaira et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Immunofluorescence microscopy of 23 kDa glutelin and 13 kDa prolamin Experiments were performed using the following methods according to Saito et al (2008).…”

Section: Cultivation Of Plants and Preparation Of Brown Rice Grainsmentioning

confidence: 99%

“…Recently, Saito et al (2008) developed a method for sectioning mature rice grains, applying the thin frozen fi lm method (Kawamoto and Shimizu, 2000) and visualized storage proteins using immunofluorescence microscopy. This method visualizes the localization of storage proteins in intact rice sections.…”

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confidence: 99%

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Analysis of Storage Protein Distribution in Rice Grain of Seed-Protein Mutant Cultivars by Immunofluorescence Microscopy

Ohdaira

Masumura

Nakatsuka

et al. 2011

Plant Production Science

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Section: Discussionmentioning

confidence: 99%

“…Immunofluorescence microscopy of 23 kDa glutelin and 13 kDa prolamin Experiments were performed using the following methods according to Saito et al (2008).…”

Section: Cultivation Of Plants and Preparation Of Brown Rice Grainsmentioning

confidence: 99%

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Analysis of Storage Protein Distribution in Rice Grain of Seed-Protein Mutant Cultivars by Immunofluorescence Microscopy

Ohdaira

Masumura

Nakatsuka

et al. 2011

Plant Production Science

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“…Saito et al (2008) 14) have developed a convenient system that uses a cryomicrotome to obtain uniform sections of the whole grain of mature seeds. Penetration of the embedding agent into the intracellular spaces of dry mature grain made it possible to achieve uniform section thickness and to conserve subcellular structures.…”

Section: )mentioning

confidence: 99%

“…The modified method used in this study allows excellent histological sections of mature cereal grains (at least for rice, oat, sorghum, and maize) to be obtained for morphometric investigation. The frozen film method 14) should be suitable for observation of tissue or cell structures and protein distribution in cereal whole grain, whereas the new method should be suitable for observation of the subcelluler structures of mature cereal grain under high magnification by optical microscope or transmission electron microscope. These methods should provide useful tools to investigate and understand cereal grains.…”

Section: )mentioning

confidence: 99%

Ultrastructure of Mature Protein Body in the Starchy Endosperm of Dry Cereal Grain

Saito

Shigemitsu

Tanaka

et al. 2010

Bioscience, Biotechnology, and Biochemistry

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Proteomic analysis of reserve proteins in commercial rice cultivars

Graziano

Marmiroli

Gullì

2020

Food Science & Nutrition

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| INTRODUC TI ONOryza sativa L. is one of the main food commodity, and nearly half of the world population depend on this culture for daily nutrition.Rice is cultivated mainly in Asia, but in Europe, Italy is the largest producer of rice with an area of 234.133 ha and a production of over 1.5 M ton, representing about 39% and 36% of the harvest area and of the European production, respectively (Faostat, December 2018). Both japonica and indica varieties are cultivated and, some japonica cultivars, such as Arborio, Carnaroli, Vialone nano, are present only in Italy. Rice consumption is steadily rising in western countries, with the adoption of new nutritional styles and the avoidance of gluten. Rice grains contain a low amount of proteins (7%-10%) most of which are storage proteins represented by glutelins, prolamins (poor in lysine), and a lower amount of albumins and globulins. The aminoacidic composition of seed storage proteins (SSP) contributes to the nutritional quality of rice seeds (Shewry and Halford, 2002). Abstract Rice consumption is rising in western countries with the adoption of new nutritional styles, which require the avoidance of gluten. Nevertheless, there are reports of rice allergic reactions. Rice grains contain a low amount of proteins most of which are storage proteins represented by glutelins, prolamins, albumins, and globulins. Some of these proteins are seed allergenic proteins as α-amylase/trypsin inhibitor, globulins, β-glyoxylase, and several glutelins. Italy is the major rice producer in Europe, and for this, seed reserve proteins of four Italian rice cultivars were characterized by 2D-GE analysis. Some differentially abundant proteins were identified and classified as allergenic proteins, prompting a further characterization of the genes encoding some of these proteins. In particular, a deletion in the promoter region of the 19 KDa globulin gene has been identified, which may be responsible for the different abundance of the protein in the Karnak cultivar. This polymorphism can be applied for cultivar identification in commercial samples. Seed proteome was characterized by a variable combination of several proteins, which may determine a different allergenic potential. Proteomic and genomic allowed to identify the protein profile of four commercial cultivars and to develop a molecular marker useful for the analysis of commercial products. K E Y W O R D S 2D-GE, allergens, Oryza sativa, seed storage proteins | 1789 GRAZIANO et Al.

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Thin Frozen Film Method for Visualization of Storage Proteins in Mature Rice Grains

Cited by 17 publications

References 9 publications

Analysis of Storage Protein Distribution in Rice Grain of Seed-Protein Mutant Cultivars by Immunofluorescence Microscopy

Analysis of Storage Protein Distribution in Rice Grain of Seed-Protein Mutant Cultivars by Immunofluorescence Microscopy

Ultrastructure of Mature Protein Body in the Starchy Endosperm of Dry Cereal Grain

Proteomic analysis of reserve proteins in commercial rice cultivars

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