Thin-layer and high-performance liquid chromatography in the evaluation of the lipophilicity of aryloxoalkanoic and arylhydroxyalkanoic acids

Kuchař, Miroslav; Kraus, E.; Jelínková, M.; Rejholec, V.; Miller, V.B.

doi:10.1016/s0021-9673(01)95502-5

Cited by 6 publications

(1 citation statement)

References 18 publications

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“…A basic physical and chemical description of the compound was carried out. The constant of lipophilicity (the logarithm of the partition coefficient-log P) was found to be in the range 6.0-6.5 by the shake flask method 30 in an octanolwater system at 20 °C. Two pK a values, pK a1 = 5.45 ± 0.15 and pK a2 = 6.69 ± 0.10 were found for VUFB-19363.…”

Section: Reagentsmentioning

confidence: 99%

On-line coupling of sequential injection extraction with restricted-access materials for sample clean-up and analysis of drugs in biological matrix

Šatı́nský

Sklenářová

Huclová

et al. 2003

Analyst

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In this contribution, the on-line coupling of solid phase extraction (SPE), based on a restricted-access material (RAM), with sequential injection technique (SIA) for the analysis of biological samples, is described. The SIA-RAM system was tested with a new potential antileucotrienic drug (VUFB-19363 (Quinlukast)) for serum analysis. The method is based on SPE with the novel internal-surface reversed-phase column packing material-alkyl-diol silica (ADS). The supports tolerate direct and repetitive injection of proteinaceous fluids (plasma, serum) and allow reversed-phase partitioning at the internal surface. A column packed with a 25 microm C18 alkyl-diol support was used for direct serum injection. Using a 6-port selection valve and the system of three mobile phases, the polar matrix compounds and metabolites are removed by sequentially aspirated mobile phases with lower content of the organic part (methanol-water (2:98) and following acetonitrile-water (20:80)) to the waste, and then, the analyte enriched on the column is eluted by a strong mobile phase (acetonitrile-methanol-water (40:20:40)) to the UV detector without transfer loss. With the fully automated SIA system, a total analysis time of less than 10 min was achieved. The only off-line sample pre-treatment step required to remove particulate matter was centrifugation. The studies showed a range of linearity (2-40 microg ml(-1)) and a high recovery (93.6-96.8%) of drug from the biological matrix with coefficients of variation (RSD) less than 5.0% (n = 6). This paper introduces a new, simple and robust analytical technique suitable for screening determination and direct analysis of drugs in biological materials.

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Section: Reagentsmentioning

confidence: 99%