Thin-layer chromatography

Abstract

The following are summaxies of papers presented at the Joint 1-day Symposium of the Western Section and the Thin-layer Chromatography Group of the Society and the Welsh College of Advanced Technology held on February 19th, 1965, and reported in the April, 1965, issue of Proceedings (p. 42).

Isolation Techniques for Flavonoids

“…Although this has not as yet been used for the separation of flavonoids, resolution with other compounds is c1aimed to be as good as TLC when low sampIe loadings (e.g. 1 : 500 (Truter, 1963)) are used.…”

“…Numerous excellent review artic1es have recently been published on the general techniques of thin-layer chromatography (TLC) (Kirchner, 1967;Stahl, 1969;Truter, 1963;Stahl and Mangold, 1967), and for this reason the comments in this artic1e will be confined largely to the types of flavonoid separation achieved by this method. TLC is a technique which has developed rapidly during the last decade and to a limited extent it has replaced paper chromatography in analytical and small scale separations of flavonoids.…”

Estimation of barbiturates by quantitative thin-layer chromatography

“…According to Truter (1963), one of the main sources of error with elution techniques in thin-layer chromatography is the elution of impurities from the adsorbent. These impurities absorb ultraviolet radiant energy, particularly from 250-380 mp, and so interfere with the spectrophotometric assay of many drugs.…”

Bloat in cattle

“…Darmstadt) coated to a thickness of 200 fL • and activated at l200e for 30 minutes. The developing solvents were chloroform-methanol-water, 80:20:2 v/v (Mangold 1962), and benzene-methanol, 85: 15 v/v (Truter 1963). The staining reagents used were iodine vapour, and 40% aqueous phosphoric acid followed by heating to 160 0 e for 20 minutes.…”