Thin‐layer chromatography and gas chromatography–mass spectrometry examination of shoe materials from patients with shoe dermatitis

Febriana, Sri Awalia; Zimerson, Erik; Svedman, Cecilia; Haryadi, Winarto; Coenraads, Pieter‐Jan; Schuttelaar, Marie L A

doi:10.1111/cod.12342

Cited by 12 publications

(6 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The area corresponding to the positive reaction on the TLC strip to which the acetone extract had been applied was analysed by scraping off the silica gel from the duplicate of the tested TLC strip and extracting the gel with a minimal amount of ethyl acetate. The obtained solution was analysed by gas chromatography-mass spectrometry (GCMS), which did not give any conclusive results (5).…”

Section: Patch Testing Thin-layer Chromatography (Tlc) and Chemical mentioning

confidence: 99%

Allergic contact dermatitis caused by dimethylthiocarbamylbenzothiazole sulfide (DMTBS) in canvas shoes: in search of the culprit allergen

et al. 2017

View full text Add to dashboard Cite

SummaryBackground. During rubber vulcanization, new compounds can be formed. Objectives. To report a case of allergic shoe dermatitis in which the search for the allergen ultimately led to the identification of dimethylthiocarbamylbenzothiazole sulfide (DMTBS). Methods. A female presented with eczema on her feet after wearing Sperry Top Sider ® canvas sneakers. Patch testing was performed with the European baseline series, additional series, shoe materials, and extracts of shoe materials. Thin-layer chromatography (TLC) was performed for additional patch testing, and high-performance liquid chromatography and gas chromatography-mass spectometry were used for chemical analysis.Results. Positive reactions were found to thiuram mix (+), tetramethylthiuram monosulfide (TMTM) (+), shoe material (+), and shoe extracts in eth. (++) and acetone (+). The extracts did not contain TMTM or other components of thiuram mix. TLC strips yielded a positive reaction (+) to one spot, whereas chemical analysis gave a negative result. Thereafter, a similar sneaker from another patient with shoe dermatitis was analysed, and DMBTS was identified. New extracts of the shoe of our first patient were then also shown to contain DMTBS. DMTBS as culprit allergen was confirmed by positive patch testing with a dilution series with DMTBS. Conclusion. DMBTS was identified as the culprit allergen in shoe dermatitis, giving rise to compound allergy. The positive reaction to TMTM was considered to represent cross-reactivity.

show abstract

Section: Patch Testing Thin-layer Chromatography (Tlc) and Chemical mentioning

confidence: 99%

Allergic contact dermatitis caused by dimethylthiocarbamylbenzothiazole sulfide (DMTBS) in canvas shoes: in search of the culprit allergen

et al. 2017

View full text Add to dashboard Cite

show abstract

“…In case 2, BIT was also present in the liquid soap that the patient came into contact with daily at work. This biocide has been reported to be present in imitation leather shoes and in certain glues used in leather shoes , and the dermatitis on the patient's feet also disappeared. However, combined foot and hand eczema is a common finding both in patients with occupational dermatitis and in those with non‐occupational contact dermatitis .…”

Section: Discussionmentioning

confidence: 83%

“…The use of this preservative is allowed in household and industrial products ; however, its use in cosmetics is not allowed . It may occasionally be present in PVC gloves and (imitation) leather shoes .…”

mentioning

confidence: 99%

Occupational allergic contact dermatitis caused by benzisothiazolinone in printing ink and soap

Meysman

Goossens

2016

Contact Dermatitis

View full text Add to dashboard Cite

“…Guidelines highlight the need to perform patch tests with both baseline and textile series when TCD is suspected, but this evidently has its limitations, such as the limited number of allergens present in commercialized patch test series, and the need to verify whether a substance to which one observes a positive patch test is effectively present in the fabric, or not 13 . Complementary chemical analyses, as exemplified by the current case, offer the possibility of identifying new textile contact allergens 1,17‐19 . Such chemical analyses may involve different techniques, like TLC and GC–MS.…”

Section: Discussionmentioning

confidence: 99%

Bikini textile contact dermatitis: A Sherlockian approach revealing 2,4‐dichlorophenol as a potential textile contact allergen

et al. 2021

View full text Add to dashboard Cite

Background: Different textile constituents may act as allergens and/or irritants and provoke textile contact dermatitis (TCD).Objectives: To report a case of TCD caused by ethylene glycol monododecyl ether and 2.4-dichlorophenol present in a bikini.Methods: A woman presented with an eczematous, pruritic rash in the area of the straps and back. Patch testing was performed with the (extended) European baseline, textile, sunscreen and photo-patch series, the bikini "as is", and ethanol and acetone extracts of the bikini. Thin-layer chromatography (TLC) of the extracts and gas chromatographymass spectrometry (GC-MS) analysis were used to elucidate the culprit(s).Result: Positive reactions were found to the bikini "as is", and to the ethanol and acetone extracts. Patch testing with TLC strips showed a strong reaction to particular fractions (3 and 4). GC-MS was performed to identify substances in each fraction, and those suspected to be skin sensitizers were patch tested. On day 4 positive reactions to ethylene glycol monododecyl ether (IR) and 2.4-dichlorophenol (++) were observed. Conclusion:A myriad of chemical compounds can be found in clothing. Ethylene glycol monododecyl ether and 2.4-dichlorophenol were identified as the potential culprits of this bikini TCD.

show abstract

Thin‐layer chromatography and gas chromatography–mass spectrometry examination of shoe materials from patients with shoe dermatitis

Abstract: A 50-year-old male with shoe dermatitis developed symmetrical eczematous lesions on the dorsal and plantarsurfaces of the feet after wearing imitation leather sandals for 6 months (Fig. 1). His lesions healed when he was not wearing the sandals, but recurred after re-exposure.

Cited by 12 publications

References 8 publications

Allergic contact dermatitis caused by dimethylthiocarbamylbenzothiazole sulfide (DMTBS) in canvas shoes: in search of the culprit allergen

Allergic contact dermatitis caused by dimethylthiocarbamylbenzothiazole sulfide (DMTBS) in canvas shoes: in search of the culprit allergen

Occupational allergic contact dermatitis caused by benzisothiazolinone in printing ink and soap

Bikini textile contact dermatitis: A Sherlockian approach revealing 2,4‐dichlorophenol as a potential textile contact allergen

Contact Info

Product

Resources

About