Thin-layer chromatography—direct bioautography as an alternative method for screening of antibiotic residues in milk: A comparative study

Piech, Tomasz; Majer‐Dziedzic, Barbara; Kostruba, Anna; Grzelak, Edyta M.; Choma, Irena

doi:10.1080/10826076.2016.1163468

Cited by 7 publications

(3 citation statements)

References 18 publications

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“…However, these methods can only estimate biological activities, which are time consuming and the results are influenced by many factors, that may lead to poor sensitivity and specificity [ 57 ]. Alternatively, high-performance liquid chromatography (HPLC), gas chromatography (GC), liquid chromatography, liquid chromatography-tandem mass spectrometry (LC-MS/MS) and capillary electrophoresis (CE) have also been applied to the detection of AGs [ 2 , 8 , 40 , 58 , 59 , 60 , 61 , 62 , 63 ]. HPLC and LC-MS/MS are the most commonly used analytical methods.…”

Section: Non-targeting Detection Methodsmentioning

confidence: 99%

Affinity-Based Analysis Methods for the Detection of Aminoglycoside Antibiotic Residues in Animal-Derived Foods: A Review

Liu

Chen

et al. 2023

Foods

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With the increasingly serious problem of aminoglycoside antibiotic residues, it is imperative to develop rapid, sensitive and efficient detection methods. This article reviews the detection methods of aminoglycoside antibiotics in animal-derived foods, including enzyme-linked immunosorbent assay, fluorescent immunoassay, chemical immunoassay, affinity sensing assay, lateral flow immunochromatography and molecular imprinted immunoassay. After evaluating the performance of these methods, the advantages and disadvantages were analyzed and compared. Furthermore, development prospects and research trends were proposed and summarized. This review can serve as a basis for further research and provide helpful references and new insights for the analysis of aminoglycoside residues. Accordingly, the in-depth investigation and analysis will certainly make great contributions to food safety, public hygiene and human health.

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Section: Non-targeting Detection Methodsmentioning

confidence: 99%

Affinity-Based Analysis Methods for the Detection of Aminoglycoside Antibiotic Residues in Animal-Derived Foods: A Review

Liu

Chen

et al. 2023

Foods

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“…In order to identify the antibiotic, various bacterial species are used, such as Bacillus stearothermophilus . This method has been optimized, particularly for the analysis of milk samples with limits of detection (LODs) between 30 and 80 ng/mL for β-lactam antibiotics, as an example [ 15 , 16 , 17 ].…”

Section: Conventional Methods Of Antibiotic Detection and Food Contam...mentioning

confidence: 99%

Electrochemical Aptasensors for Antibiotics Detection: Recent Achievements and Applications for Monitoring Food Safety

Evtugyn

Porfireva

Tsekenis

et al. 2022

Sensors

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Antibiotics are often used in human and veterinary medicine for the treatment of bacterial diseases. However, extensive use of antibiotics in agriculture can result in the contamination of common food staples such as milk. Consumption of contaminated products can cause serious illness and a rise in antibiotic resistance. Conventional methods of antibiotics detection such are microbiological assays chromatographic and mass spectroscopy methods are sensitive; however, they require qualified personnel, expensive instruments, and sample pretreatment. Biosensor technology can overcome these drawbacks. This review is focused on the recent achievements in the electrochemical biosensors based on nucleic acid aptamers for antibiotic detection. A brief explanation of conventional methods of antibiotic detection is also provided. The methods of the aptamer selection are explained, together with the approach used for the improvement of aptamer affinity by post-SELEX modification and computer modeling. The substantial focus of this review is on the explanation of the principles of the electrochemical detection of antibiotics by aptasensors and on recent achievements in the development of electrochemical aptasensors. The current trends and problems in practical applications of aptasensors are also discussed.

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“…In order to ensure food is safe for human consumption, the European Union (EU) has set the maximum residue limits (MRLs) for doxycycline as 0.1mg kg -1 in mussel, 0.3 mg kg -1 in skin and fat, 0.3 mg kg -1 in the liver, and 0.6 mg kg-1 in the kidney, respectively [23]. To date, several techniques have been used for the analysis of doxycycline residues in food samples, such as highperformance liquid chromatography [24,25], thin-layer chromatography [26,27], capillary electrophoresis [28,29], immunoassays (e.g. ELISA) [30,31].…”

Section: Introductionmentioning

confidence: 99%

Fluorometric determination of doxycycline based on the use of carbon quantum dots incorporated into a molecularly imprinted polymer

et al. 2018

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Thin-layer chromatography—direct bioautography as an alternative method for screening of antibiotic residues in milk: A comparative study

Cited by 7 publications

References 18 publications

Affinity-Based Analysis Methods for the Detection of Aminoglycoside Antibiotic Residues in Animal-Derived Foods: A Review

Affinity-Based Analysis Methods for the Detection of Aminoglycoside Antibiotic Residues in Animal-Derived Foods: A Review

Electrochemical Aptasensors for Antibiotics Detection: Recent Achievements and Applications for Monitoring Food Safety

Fluorometric determination of doxycycline based on the use of carbon quantum dots incorporated into a molecularly imprinted polymer

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