Thin-layer chromatography of organophosphorus pesticides and acids on microchromatoplates

Stanley, Charles W.

doi:10.1016/s0021-9673(01)82517-6

Cited by 33 publications

(7 citation statements)

References 10 publications

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“…Bidimensional thin-layer chromatography of phospholipids was made on glass plates (Silica Gel 60) according to Awasthi et al [31]. Phospholipids were visualized either by spraying with ammonium molybdate/HC104 [32] or with iodine vapor. The iodine-stained spots were scraped out and phosphate was determined according to Schnitger et al [33].…”

Section: Methodsmentioning

confidence: 99%

Kinetic and Structural Differences between Cytochrome c Oxidases from Beef Liver and Heart

Merle

Kadenbach

1982

European Journal of Biochemistry

106

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The cytochrome content of beef liver mitochondria differs from that of beef heart mitochondria by an eight‐ fold lower cytochrome aa3 and a twofold lower cytochrome b and c + c1 content. The kinetic properties of cytochrome c oxidases from beef liver and heart were measured with intact cytochrome c‐depleted membranes, deoxycholate‐dissolved membranes, and with the isolated enzymes at various cytochrome c concentrations with an oxygen electrode. Under all conditions a higher V was found for the liver enzyme, both for the low‐affinity and for the high‐affinity binding site for cytochrome c. Differences were also found for the Km of the two enzymes. Isolated beef heart mitochondria contained about twice as much cardiolipin than beef liver mitochondria. The isolated enzymes contained one mole cardiolipin per mole of the heart enzyme, but 2 moles cardiolipin per mole of the liver enzyme. By application of a high performance sodium dodecylsulfate gel electrophoretic system the two isolated enzymes could be separated into 13 different protein components, three of which (polypeptides VIa, VIIa and VIII) were found to differ in their apparent molecular weights. The functional meaning of cytochrome c oxidase iso‐ enzymes in liver and heart is discussed.

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Section: Methodsmentioning

confidence: 99%

Kinetic and Structural Differences between Cytochrome c Oxidases from Beef Liver and Heart

Merle

Kadenbach

1982

European Journal of Biochemistry

106

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“…The silver nitrate procedure used was identical to the thin-Iayer mOOification described by WALKER and BEROZA (1963). STANLEY (1964) found that the fluorescein method was most effective when the plates were first sprayed with the iodine spray reagent, spots were recorded, and then the plates were oversprayed with fluorescein solution as described by McKINLEY and MAHON (1959). Some spots were visible under room light but others produced spots only after exposure to 2547 A or 3660A ultraviolet light.…”

Section: Thin-iayer Chromatography -Chemical Detectionmentioning

confidence: 99%

“…Spots were then observed under either 2547 A or 3660A ultraviolet light. STANLEY (1964) modified the reagent used by HANES and ISHERWOOD (1949) for use with thin-Iayer plates. The modified reagent was prepared by dissolving 0.5 g. of ammonium molybdate tetrahydrate in live ml.…”

Section: Thin-iayer Chromatography -Chemical Detectionmentioning

confidence: 99%

Residue Reviews / Rückstands-Berichte

1967

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“…A number of papers have also been published on the thinlayer chromatographic analyses of organophosphorus pesticides (Abbott et al, 1967;Bunyan, 1964;Ragab, 1967a,b;Salame, 1964;Stanley, 1964;Walker and Beroza, 1963). A methodology for the determination of phosalone and its oxygen analog is presented.…”

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confidence: 99%