Thin-layer chromatography of polymers introductory lecture

Belen'kii, B.G.; Gankina, E.S.

doi:10.1016/s0021-9673(00)86699-6

Cited by 45 publications

(7 citation statements)

References 12 publications

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“…Then tubes were placed on a hot plate and stirred with medium speed at 37 °C. Aliquots of 10 mL were withdrawn at different time points (1,2,4,12,24,36, and 48 h) and replaced the same with an equal volume of fresh buffers. The released fluoxetine concentration was analyzed through a UV-vis spectrophotometer at a wavelength of 227 nm.…”

Section: In-vitro Drug Releasementioning

confidence: 99%

Fabrication, Characterization and Therapeutic Evaluation of Fluoxetine‐Dextran Nanoparticles

et al. 2023

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In recent times conventional dosage forms face a number of challenges, i. e., adverse effects, low absorption of drugs, and delivery at the targeted sites for desired therapeutic effect. In the current study, dextran-fluoxetine nanoconjugates were prepared, characterized, and evaluated for therapeutic efficacy. Dextran was oxidized via aldehyde functional group subsequently conjugated with fluoxetine using Schiff based reaction. Characterization techniques like ultra-violet spectroscopy, Fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, and circular dichroism spectroscopy confirmed successful drug-polymer conjugation. Nanoconjugates size and shape was assessed using dynamic light scattering and atomic force microscopy respectively, whereas thermo-gravimetric analysis revealed the stability. Drug loading was found 21 %, whereas the drug release for 48 h showed 28 % release at pH = 5 and 16 % at pH = 7.4. Anticancer activity of the nanoparticles was assessed using HeLa cell lines and revealed 99.5 % cellular inhibition whereas cytotoxic assay was done using healthy cell line 3T3 showing 14.6 % cell inhibition. This study showed controlled release of drugs from the nanoparticles in the simulated conditions and has potential to reduce associated adverse effects. It is hypothesized that these nanoparticles may find application in anticancer drugs-controlled release with reduced toxicities.

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Section: In-vitro Drug Releasementioning

confidence: 99%

Fabrication, Characterization and Therapeutic Evaluation of Fluoxetine‐Dextran Nanoparticles

et al. 2023

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“…From these investigations occasional molec ular weight effects were noted (8). Recently efforts in three separate laboratories have been successful in determining the molecular weight distribution of a polymer sample by TLC techniques (9)(10)(11)(12). An explana tion of the facility and high resolving power observed has been sought through a number of continuing experiments (11)(12)(13)(14)(15)(16).…”

Section: Thin-layer Chromatography (Tlc) Is a Common Laboratory Technmentioning

confidence: 99%

“…Recently efforts in three separate laboratories have been successful in determining the molecular weight distribution of a polymer sample by TLC techniques (9-12). An explana tion of the facility and high resolving power observed has been sought through a number of continuing experiments (11)(12)(13)(14)(15)(16). The purpose of this report is to review the results of these studies and to comment on the fractionation mechanisms postulated.…”

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confidence: 98%

Thin-Layer Methods for Determining Molecular Weight Distribution

Otocka¹

1973

Polymer Molecular Weight Methods

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Thin-layer chromatography (TLC) is a common laboratory technique for separating complex mixtures of solutes, usually by an adsorbtion mechanism. Several laboratories have applied the technique to the separation of polymer fractions and characterization of polymer molecular weight distribu tions. This work reviews the experimental results and theoretical approaches to the fractionation mechanisms.^phin-layer chromatography (TLC) is a well-known technique for the separation of mixed solutes by adsorption. Compared with related techniques in column chromatography, TLC offers several advantages. The use of adsorbents having large surface area ( particle size ~10 μ vs. 50 μ) results in excellent resolution. Separation and analysis time are reduced because many samples can be run simultaneously and material need not be eluted for quantitation. Several references provide a com prehensive background on TLC ( 1-3 ).TLC has been a widely accepted technique in biochemistry and or ganic chemistry for a number of years. With modern quantitation TLC is being used today in such diverse applications as air pollution analysis and clinical medicine.The initial applications of TLC to problems in polymer chemistry were directed to the separation of polymer blends, stereoisomers, and a variety of copolymers ( 4-8 ). From these investigations occasional molec ular weight effects were noted (8). Recently efforts in three separate laboratories have been successful in determining the molecular weight distribution of a polymer sample by TLC techniques (9-12). An explana tion of the facility and high resolving power observed has been sought through a number of continuing experiments (11)(12)(13)(14)(15)(16). The purpose of this report is to review the results of these studies and to comment on the fractionation mechanisms postulated. 55Downloaded by UCSF LIB CKM RSCS MGMT on December 2, 2014 |

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“…TLC does not necessitate any expensive instruments and can be practiced easily. Validity of TLC in polymer chemistry has already been verified by some researchers (6,7,8). Kieselgei H (E. Merk Typ 60; layer thickness, 0.4 ram).was used as a stationary phase.…”

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confidence: 91%