Thin-layer chromatography of steroids

Applications of Thin-Layer Chromatography in Pharmaceutical Analyses

“…Heftmann 's (167) article on T L C of steroids in chromatographic reviews (13 pages, 180 references) again surveyed earlier work and extended the review Methyltestosterone and three related compounds were extracted from tablets and capsules with acetone and from liquids with CHC13 for subsequent TLC by Castren (183). After development in benzene-ethyl acetate (7 : 3), the methyltestosterone 2,4-dinitrophenylhydrazone was determined colorimetrically with accuracy Jacobsohn (184) photographed the H2S04 color reaction zones of TLC plates of estrone, estradiol, and estriol, irradiated by U.V.…”

“…The section on steroids by Waldi in Stahl's book (1) followed by a series (13 pages) by Neher in Marini-Bettolo's book (22) on TLC of steroids and by Cavina and Vicari in Marini-Bettolo's book (22) on the qualitative and quantitative analyses of natural and synthetic corticosteroids by T L C (14 pages) covered the literature through 1962-1963. These authors all gave excellent details on techniques, reagents, and listings for specific separation. Heftmann 's (167) article on T L C of steroids in chromatographic reviews (13 pages, 180 references) again surveyed earlier work and extended the review Methyltestosterone and three related compounds were extracted from tablets and capsules with acetone and from liquids with CHC13 for subsequent TLC by Castren (183). After development in benzene-ethyl acetate (7 : 3), the methyltestosterone 2,4-dinitrophenylhydrazone was determined colorimetrically with accuracy Jacobsohn (184) photographed the H2S04 color reaction zones of TLC plates of estrone, estradiol, and estriol, irradiated by U.V.…”

Chromatography

“…General surveys of steroid chromatography have been written by Heftmann and Wotiz (770) and by Seki (1834). PC was reviewed by Siblikova (1871) and TLC by several other authors (736,769,1413,1988,2049,2108). More specialized applications which have been reviewed follow: chromatography of sterols (335), of cholesterol and its esters (957), and of bile acids (1892); TLC of bile acids (807,965), of steroid hormones (849), and of adrenocortical hormones (1428); PC of steroid glycosides (915) and of digitalis glycosides (2218); TLC of sapogenins (1714), cardenolides (1464), and bufadienolides (1468).…”