1973
DOI: 10.1016/0003-2697(73)90114-0
|View full text |Cite
|
Sign up to set email alerts
|

Thin-layer chromatography of sub-nanomole amounts of phenylthiohydantoin (PTH) amino acids on polyamide sheets

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
96
0
1

Year Published

1974
1974
1983
1983

Publication Types

Select...
9
1

Relationship

0
10

Authors

Journals

citations
Cited by 349 publications
(97 citation statements)
references
References 2 publications
0
96
0
1
Order By: Relevance
“…This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. Amino acid sequence analysis of the fully reduced and alkylated protein (24) was carried out in the automatic sequencer (Beckman 890C) using the Quadrol double cleavage program. Residues released were identified by thin-layer chromatography (25), by back hydrolysis in 6 M HCI under reduced pressure for 24 hr at 1500 followed by amino acid analysis, and by colorimetric assay (His and Arg) (26,27). Three separate determinations made on separately prepared samples, two from milk and one from colostrum, gave equivalent results.…”
mentioning
confidence: 99%
“…This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. Amino acid sequence analysis of the fully reduced and alkylated protein (24) was carried out in the automatic sequencer (Beckman 890C) using the Quadrol double cleavage program. Residues released were identified by thin-layer chromatography (25), by back hydrolysis in 6 M HCI under reduced pressure for 24 hr at 1500 followed by amino acid analysis, and by colorimetric assay (His and Arg) (26,27). Three separate determinations made on separately prepared samples, two from milk and one from colostrum, gave equivalent results.…”
mentioning
confidence: 99%
“…All PTH amino acids were analyzed by gas chromatography [20] and thin layer chromatography [21], and 14C was counted in a Packard scintillation spectrometer Model 3003.…”
Section: Methodsmentioning
confidence: 99%
“…Protamine was introduced into the cup in water and subjected to 34 successive cycles of Edman degradation. Phenylthiohydantoins were analysed by HPLC on a Beckman HPLC instrument with a Zorbax (Du Pont) cyanopropyl column according to the method of Johnson et al [13], with norleucine as an internal standard; they were also identified by chromatography on polyamide sheets [14].…”
Section: Automuted Protein Sequencingmentioning
confidence: 99%