Thin Pilus PilV Adhesins of Plasmid R64 Recognize Specific Structures of the Lipopolysaccharide Molecules of Recipient Cells

Ishiwa, Akiko; Komano, Teruya

doi:10.1128/jb.185.17.5192-5199.2003

Cited by 30 publications

(39 citation statements)

References 27 publications

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“…This interaction results in stabilization of conjugation partners and is necessary for efficient mobilization (45). Adhesin PilV of the thin pilus encoded by plasmid R64 specifically interacts with the lipopolysaccharide of the recipient cells, determining recipient specificity (46,47). Receptors similar to OmpA or specific lipopolysaccharide components might be lacking in cyanobacteria and thus prevent the interphylum conjugation of MPF F (F) or MPF I (R64) plasmids, this being a second alternative to explain MPF T enhanced promiscuity.…”

Section: Discussionmentioning

confidence: 99%

Plasmid Conjugation from Proteobacteria as Evidence for the Origin of Xenologous Genes in Cyanobacteria

et al. 2014

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Comparative genomics have shown that 5% of Synechococcus elongatus PCC 7942 genes are of probable proteobacterial origin. To investigate the role of interphylum conjugation in cyanobacterial gene acquisition, we tested the ability of a set of prototype proteobacterial conjugative plasmids (RP4, pKM101, R388, R64, and F) to transfer DNA from Escherichia coli to S. elongatus. A series of BioBrick-compatible, mobilizable shuttle vectors was developed. These vectors were based on the putative origin of replication of the Synechococcus resident plasmid pANL. Not only broad-host-range plasmids, such as RP4 and R388, but also narrower-host-range plasmids, such as pKM101, all encoding MPF T -type IV secretion systems, were able to transfer plasmid DNA from E. coli to S. elongatus by conjugation. Neither MPF F nor MPF I could be used as interphylum DNA delivery agents. Reciprocally, pANL-derived cointegrates could be introduced in E. coli by electroporation, where they conferred a functional phenotype. These results suggest the existence of potentially ample channels of gene flow between proteobacteria and cyanobacteria and point to MPF T -based interphylum conjugation as a potential mechanism to explain the proteobacterial origin of a majority of S. elongatus xenologous genes.

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Section: Discussionmentioning

confidence: 99%

Plasmid Conjugation from Proteobacteria as Evidence for the Origin of Xenologous Genes in Cyanobacteria

et al. 2014

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“…The thin pilus locus encompasses 14 genes, designated pilI through pilV, and 12 of these genes are essential for pilus biogenesis (49). A number of studies define the role of the thin pili in mediating surface interactions between bacteria (16)(17)(18); however, the IncI1-encoded type IV pilus has not been implicated in phenotypes apart from conjugation.…”

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confidence: 99%

An IncI1 Plasmid Contributes to the Adherence of the Atypical EnteroaggregativeEscherichia coliStrain C1096 to Cultured Cells and Abiotic Surfaces

et al. 2006

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Enteroaggregative Escherichia coli (EAEC) is defined by a characteristic "stacked-brick" aggregative adherence (AA) pattern to cultured cells. In well-studied EAEC prototype strains (called typical EAEC strains), the AA phenotype requires aggregative adherence fimbriae (AAFs). However, previous studies suggest that known AAF alleles are not found in all EAEC strains. To define mechanisms contributing to adherence in an atypical strain, we studied EAEC strain C1096. An E. coli K12 derivative carrying two plasmids, designated pSERB1 and pSERB2, from C1096 adhered to cell lines and exhibited an AA pattern. Nucleotide sequence analysis of pSERB1 indicated that it is related to plasmids of the IncI1 incompatibility group. These plasmids encode genes involved in pilus-mediated conjugal transfer, as well as pilL-V, which encodes a second pilus of the type IV family. Insertional inactivation of the gene predicted to encode the major type IV pilin subunit (pilS) reduced conjugal transfer of the plasmid by 4 orders of magnitude. Adherence of the mutant strain to polystyrene and to HT29 cells was reduced by approximately 21% and 75%, respectively. In a continuous-flow microfermentor, the pilS inactivation reduced mature biofilm formation on a glass slide by approximately 50%. In addition, the simultaneous presence of both pSERB1 and pSERB2 plasmids promoted pilS-independent biofilm formation. We conclude that the IncI1 plasmid of EAEC C1096 encodes a type IV pilus that contributes to plasmid conjugation, epithelial cell adherence, and adherence to abiotic surfaces. We also observe that AA can be mediated by factors distinct from AAF adhesins.

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“…The seven PilV C-terminal segments determine the recipient specificity of R64 liquid mating through recognition of lipopolysaccharides (LPSs) of recipient cells (10). The recipient E. coli K-12 strain was recognized by the PilVAЈ, PilVC, and PilVCЈ adhesins.…”

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confidence: 99%

“…E. coli R1, S. enterica serovar Typhimurium LT2, and Shigella flexneri were recognized by the PilVA or PilVAЈ, PilVAЈ or PilVBЈ, and PilVAЈ or PilVDЈ adhesin, respectively. Liquid mating experiments using various waa mutants showed that the specific receptor structures for the PilVA, PilVBЈ, PilVC, and PilVCЈ adhesins are GlcNAc(␤1-3)Glc, GlcNAc(␣1-2)Glc, GlcNAc(␤1-7)Hep, and Glc(␣1-2)Glc or Glc(␣1-2)Gal, respectively, of LPS from E. coli R1, E. coli K-12, or S. enterica serovar Typhimurium LT2 (10,11). The binding of PilV adhesins or their C-terminal segments to LPSs has been demonstrated using filter overlay assays and surface plasmon resonance analyses (11).…”

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confidence: 99%

Novel Class of Mutations of pilS Mutants, Encoding Plasmid R64 Type IV Prepilin: Interface of PilS-PilV Interactions

et al. 2008

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The type IV pili of plasmid R64 belonging to the type IVB group are required only for liquid mating. They consist of the major and minor components PilS pilin and PilV adhesin, respectively. PilS pilin is first synthesized as a 22-kDa prepilin from the pilS gene and is then processed to a 19-kDa mature pilin by PilU prepilin peptidase. In a previous genetic analysis, we identified four classes of the pilS mutants (T. Horiuchi and T. Komano, J. Bacteriol. 180:4613-4620, 1998). The products of the class I pilS mutants were not processed by prepilin peptidase; the products of the class II mutants were not secreted; in the class III mutants type IV pili with reduced activities in liquid mating were produced; and in the class IV mutants type IV pili with normal activities were produced. Here, we describe a novel class, class V, of pilS mutants. Mutations in the pilS gene at Gly-56 or Tyr-57 produced type IV pili lacking PilV adhesin, which were inactive in liquid mating. Residues 56 and 57 of PilS pilin are suggested to function as an interface of PilS-PilV interactions.

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Thin Pilus PilV Adhesins of Plasmid R64 Recognize Specific Structures of the Lipopolysaccharide Molecules of Recipient Cells

Cited by 30 publications

References 27 publications

Plasmid Conjugation from Proteobacteria as Evidence for the Origin of Xenologous Genes in Cyanobacteria

Plasmid Conjugation from Proteobacteria as Evidence for the Origin of Xenologous Genes in Cyanobacteria

An IncI1 Plasmid Contributes to the Adherence of the Atypical EnteroaggregativeEscherichia coliStrain C1096 to Cultured Cells and Abiotic Surfaces

Novel Class of Mutations of pilS Mutants, Encoding Plasmid R64 Type IV Prepilin: Interface of PilS-PilV Interactions

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