Thiocapsa marina sp. nov., a novel, okenone-containing, purple sulfur bacterium isolated from brackish coastal and marine environments

Cartigny, Pierre; Guyoneaud, Rémy; Imhoff, Johannes F.; Süling, Jörg; Горленко, В. М.

doi:10.1099/ijs.0.02964-0

Cited by 51 publications

(30 citation statements)

References 21 publications

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“…With its independence of vitamins, its salt tolerance and high tolerance towards oxygen, strain KS1 is a typical representative of the genus Thiocapsa (Puchkova et al, 2000;Caumette et al, 2004). Also, two known strains of T. roseopersicina, DSM 221 and DSM 217, were found to oxidize nitrite phototrophically.…”

Section: Discussionmentioning

confidence: 99%

“…Photosynthetic bacteria can be found in almost every aquatic environment, including freshwater, marine, alkaline, acidic and hot or cold waters (Pfennig, 1976(Pfennig, , 1978Stanier et al, 1981;Trüper & Pfennig, 1981;Van Trappen et al, 2004;Caumette et al, 2004;Herbert et al, 2005;Asao et al, 2007). In addition to these natural environments, purple anoxygenic phototrophs also inhabit engineered systems, such as wastewater treatment facilities, although their role there is not well understood.…”

Section: Introductionmentioning

confidence: 99%

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Anaerobic phototrophic nitrite oxidation by Thiocapsa sp. strain KS1 and Rhodopseudomonas sp. strain LQ17

2010

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In anaerobic enrichment cultures for phototrophic nitrite-oxidizing bacteria from different freshwater sites, two different cell types, i.e. non-motile cocci and motile, rod-shaped bacteria, always outnumbered all other bacteria. Most-probable-number (MPN) dilution series with samples from two freshwater sites yielded only low numbers (¡3¾10 3 cm "3 ) of phototrophic nitrite oxidizers. Slightly higher numbers (about 10 4 cm "3 ) were found in activated sewage sludge. Anaerobic phototrophic oxidation of nitrite was studied with two different isolates, the phototrophic sulfur bacterium strain KS1 and the purple nonsulfur bacterium strain LQ17, both of which were isolated from activated sludge collected from the municipal sewage treatment plant in Konstanz, Germany. Strain KS1 converted 1 mM nitrite stoichiometrically to nitrate with concomitant formation of cell matter within 2-3 days, whereas strain LQ17 oxidized only up to 60 % of the given nitrite to nitrate within several months with the concomitant formation of cell biomass. Nitrite oxidation to nitrate was strictly light-dependent and required the presence of molybdenum in the medium. Nitrite was oxidized in both the presence and absence of oxygen. Nitrite inhibited growth at concentrations higher than 2 mM. Hydroxylamine and hydrazine were found to be toxic to the phototrophs in the range 5-50 mM and did not stimulate phototrophic growth. Based on morphology, substrate-utilization pattern, in vivo absorption spectra, and 16S rRNA gene sequence similarity, strain KS1 was assigned to the genus Thiocapsa and strain LQ17 to the genus Rhodopseudomonas. Also, Thiocapsa roseopersicina strains DSM 217 and DSM 221 were found to oxidize nitrite to nitrate with concomitant growth. We conclude that the ability to use nitrite phototrophically as electron donor is widespread in nature, but low MPN counts indicate that its contribution to nitrite oxidation in the studied habitats is rather limited.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anaerobic phototrophic nitrite oxidation by Thiocapsa sp. strain KS1 and Rhodopseudomonas sp. strain LQ17

2010

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“…In the case of the soeABC genes, a function for the encoded proteins has been reported but not rigorously documented (Lehmann et al, 2012). References for substrate ranges: Bryantseva et al (1999), Zaar et al (2003), Caumette et al (2004) and Imhoff (2005a, b).…”

Section: Resultsmentioning

confidence: 99%

Sulfite oxidation in the purple sulfur bacterium Allochromatium vinosum: identification of SoeABC as a major player and relevance of SoxYZ in the process

et al. 2013

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In phototrophic sulfur bacteria, sulfite is a well-established intermediate during reduced sulfur compound oxidation. Sulfite is generated in the cytoplasm by the reverse-acting dissimilatory sulfite reductase DsrAB. Many purple sulfur bacteria can even use externally available sulfite as a photosynthetic electron donor. Nevertheless, the exact mode of sulfite oxidation in these organisms is a long-standing enigma. Indirect oxidation in the cytoplasm via adenosine-59-phosphosulfate (APS) catalysed by APS reductase and ATP sulfurylase is neither generally present nor essential. The inhibition of sulfite oxidation by tungstate in the model organism Allochromatium vinosum indicated the involvement of a molybdoenzyme, but homologues of the periplasmic molybdopterin-containing SorAB or SorT sulfite dehydrogenases are not encoded in genome-sequenced purple or green sulfur bacteria. However, genes for a membrane-bound polysulfide reductase-like iron-sulfur molybdoprotein (SoeABC) are universally present. The catalytic subunit of the protein is predicted to be oriented towards the cytoplasm. We compared the sulfide-and sulfite-oxidizing capabilities of A. vinosum WT with single mutants deficient in SoeABC or APS reductase and the respective double mutant, and were thus able to prove that SoeABC is the major sulfite-oxidizing enzyme in A. vinosum and probably also in other phototrophic sulfur bacteria. The genes also occur in a large number of chemotrophs, indicating a general importance of SoeABC for sulfite oxidation in the cytoplasm. Furthermore, we showed that the periplasmic sulfur substrate-binding protein SoxYZ is needed in parallel to the cytoplasmic enzymes for effective sulfite oxidation in A. vinosum and provided a model for the interplay between these systems despite their localization in different cellular compartments.

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“…Synechoxanthin and related compounds (see Table 2) are especially abundant in the stationary phase in those cyanobacterial cells that can synthesize them, are apparently associated predominantly with the outer membrane and spent growth medium, and have absorption properties in solution that suggest they might act as UV-absorbing sunscreen compounds. Some of the phototrophic purple sulfur bacteria (PSB), members of the ''Chromatiales,'' produce okenone as the primary carotenoid (e.g., Caumette et al 1991Caumette et al , 2004Kumar et al 2007;Sidorova et al 1998). Okenone is a monocyclic aromatic carotenoid with one v-ring end group; the arrangement of the methyl groups is identical to that of renierapurpurin, the precursor to synechoxanthin.…”

Section: Ring Desaturationsmentioning

confidence: 99%

The biochemical basis for structural diversity in the carotenoids of chlorophototrophic bacteria

2008

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Ongoing work has led to the identification of most of the biochemical steps in carotenoid biosynthesis in chlorophototrophic bacteria. In carotenogenesis, a relatively small number of modifications leads to a great diversity of carotenoid structures. This review examines the individual steps in the pathway, discusses how each contributes to structural diversity among carotenoids, and summarizes recent progress in elucidating the biosynthetic pathways for carotenoids in chlorophototrophs.

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Thiocapsa marina sp. nov., a novel, okenone-containing, purple sulfur bacterium isolated from brackish coastal and marine environments

Abstract: Thiocapsa marina sp. nov., a novel, okenonecontaining, purple sulfur bacterium isolated from brackish coastal and marine environments

Cited by 51 publications

References 21 publications

Anaerobic phototrophic nitrite oxidation by Thiocapsa sp. strain KS1 and Rhodopseudomonas sp. strain LQ17

Anaerobic phototrophic nitrite oxidation by Thiocapsa sp. strain KS1 and Rhodopseudomonas sp. strain LQ17

Sulfite oxidation in the purple sulfur bacterium Allochromatium vinosum: identification of SoeABC as a major player and relevance of SoxYZ in the process

The biochemical basis for structural diversity in the carotenoids of chlorophototrophic bacteria

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