Thiohalocapsa marina sp. nov., from an Indian marine aquaculture pond

Kumar, P. Anil; Srinivas, T. N. R.; Thiel, Vera; Tank, Marcus; Sasikala, Ch.; Ramana, Ch. V.; Imhoff, Johannes F.

doi:10.1099/ijs.0.003053-0

Cited by 14 publications

(8 citation statements)

References 18 publications

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“…Using these final atomic ratios, our data showed that Thiohalocapsa -related bacteria preferentially assimilate acetate and bicarbonate in the light. Their uptakes of acetate, bicarbonate and pyruvate under light conditions are in agreement with the substrate utilization of Thiohalocapsa species in culture under anaerobic conditions when sulfide is present ( Caumette et al, 1991 ; Imhoff et al, 1998 ; Anil Kumar et al, 2009 ), suggesting that anoxic conditions prevailed in the mats during the incubation experiments. To our knowledge, no studies have compared the assimilation rates of carbon sources by Thiohalocapsa species either in culture or in the natural environment.…”

Section: Discussionsupporting

confidence: 72%

“…Second, Thiohalocapsa -related sequences were prominent in the DNA- and rRNA based pufM libraries. And third, microcolonies viewed on NanoSIMS images were mainly composed by spherical cells typical of the Thiohalocapsa species described to date ( Caumette et al, 1991 ; Imhoff et al, 1998 ; Anil Kumar et al, 2009 ). Minor phylotypes of purple sulfur bacteria and aerobic anoxygenic phototrophic bacteria were detected in the mat samples.…”

Section: Discussionmentioning

confidence: 91%

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A Nanoscale Study of Carbon and Nitrogen Fluxes in Mats of Purple Sulfur Bacteria: Implications for Carbon Cycling at the Surface of Coastal Sediments

et al. 2017

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Mass blooms of purple sulfur bacteria growing seasonally on green stranded macroalgae have a major impact on the microbial composition and functionality of intertidal mats. To explore the active anoxygenic phototrophic community in purple bacterial mats from the Roscoff Aber Bay (Brittany, France), we conducted a combined approach including molecular and high-resolution secondary ion mass spectrometry (NanoSIMS) analyses. To investigate the dynamics of carbon and nitrogen assimilation activities, NanoSIMS was coupled with a stable isotope probing (SIP) experiment and a compound specific isotope analysis (CSIA) of fatty acid methyl ester (FAME). Sediment samples were incubated with 13C- and/or 15N-labeled acetate, pyruvate, bicarbonate and ammonium. NanoSIMS analysis of 13C - and 15N -incubated samples showed elevated incorporations of 13C - and 15N in the light and of 13C -acetate in the dark into dense populations of spherical cells that unambiguously dominated the mats. These results confirmed CSIA data that ranked vaccenic acid, an unambiguous marker of purple sulfur bacteria, as the most strongly enriched in the light after 13C -acetate amendment and indicated that acetate uptake, the most active in the mat, was not light-dependent. Analysis of DNA- and cDNA-derived pufM gene sequences revealed that Thiohalocapsa-related clones dominated both libraries and were the most photosynthetically active members of the mat samples. This study provides novel insights into the contribution of purple sulfur bacteria to the carbon cycle during their seasonal developments at the sediment surface in the intertidal zone.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 91%

A Nanoscale Study of Carbon and Nitrogen Fluxes in Mats of Purple Sulfur Bacteria: Implications for Carbon Cycling at the Surface of Coastal Sediments

et al. 2017

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“…On the other hand, in families that are phenotypically well studied, like Chromatiaceae ( e.g. , [53]) or Enterobacteriaceae [54] the delineation of genera is often based on 16S rRNA gene divergence values of around 3% or less. However, the determined significant phenotypic differences among closely related strains within the OM60/NOR5 clade indicate that comparative 16S rRNA sequence analyses alone do not allow a reliable dissection of taxa in this phylogenetic group.…”

Section: Resultsmentioning

confidence: 99%

Taxonomy and evolution of bacteriochlorophyll a-containing members of the OM60/NOR5 clade of marine gammaproteobacteria: description of Luminiphilus syltensis gen. nov., sp. nov., reclassification of Haliea rubra as Pseudohaliea rubra gen. nov., comb. nov., and emendation of Chromatocurvus halotolerans

et al. 2013

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BackgroundAerobic gammaproteobacteria affiliated to the OM60/NOR5 clade are widespread in saline environments and of ecological importance in several marine ecosystems, especially the euphotic zone of coastal areas. Within this group a close relationship between aerobic anoxygenic photoheterotrophs and non-phototrophic members has been found.ResultsSeveral strains of aerobic red-pigmented bacteria affiliated to the OM60/NOR5 clade were obtained from tidal flat sediment samples at the island of Sylt (North Sea, Germany). Two of the novel isolates, Rap1red and Ivo14T, were chosen for an analysis in detail. Strain Rap1red shared a 16S rRNA sequence identity of 99% with the type strain of Congregibacter litoralis and was genome-sequenced to reveal the extent of genetic microheterogeneity among closely related strains within this clade. In addition, a draft genome sequence was obtained from the isolate Ivo14T, which belongs to the environmental important NOR5-1 lineage that contains so far no cultured representative with a comprehensive description. Strain Ivo14T was characterized using a polyphasic approach and compared with other red-pigmented members of the OM60/NOR5 clade, including Congregibacter litoralis DSM 17192T, Haliea rubra DSM 19751T and Chromatocurvus halotolerans DSM 23344T. All analyzed strains contained bacteriochlorophyll a and spirilloxanthin as photosynthetic pigments. Besides a detailed phenotypic characterization including physiological and chemotaxonomic traits, sequence information based on protein-coding genes and a comparison of draft genome data sets were used to identify possible features characteristic for distinct taxa within this clade.ConclusionsComparative sequence analyses of the pufLM genes of genome-sequenced representatives of the OM60/NOR5 clade indicated that the photosynthetic apparatus of these species was derived from a common ancestor and not acquired by multiple horizontal gene transfer from phylogenetically distant species. An affiliation of the characterized bacteriochlorophyll a-containing strains to different genera was indicated by significant phenotypic differences and pufLM nucleotide sequence identity values below 82%. The revealed high genotypic and phenotypic diversity of closely related strains within this phylogenetic group reflects a rapid evolution and frequent niche separation in the OM60/NOR5 clade, which is possibly driven by the necessities of an adaptation to oligotrophic marine habitats.

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“…The cellular sulfur of Chromatiaceae species is dominated by periplasmic stores of elemental sulfur (34% of total cell weight), which is exclusively derived from the reduced sulfur pool ( Dahl and Prange, 2006 ). Furthermore, many Chromatiaceae , including the closest cultured relatives to PB-PSB1, are unable to assimilate sulfate ( Dahl, 2008 ; Kumar et al , 2009 ). Even in those species that can assimilate sulfate, this process is thought to be repressed during anoxic photolithotrophic growth ( Neumann et al , 2000 ; Sander and Dahl, 2008 ).…”

Section: Discussionmentioning

confidence: 99%

Microscale sulfur cycling in the phototrophic pink berry consortia of the Sippewissett Salt Marsh

Wilbanks

Jaekel

Salman

et al. 2014

Environmental Microbiology

116

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Microbial metabolism is the engine that drives global biogeochemical cycles, yet many key transformations are carried out by microbial consortia over short spatiotemporal scales that elude detection by traditional analytical approaches. We investigate syntrophic sulfur cycling in the ‘pink berry’ consortia of the Sippewissett Salt Marsh through an integrative study at the microbial scale. The pink berries are macroscopic, photosynthetic microbial aggregates composed primarily of two closely associated species: sulfide-oxidizing purple sulfur bacteria (PB-PSB1) and sulfate-reducing bacteria (PB-SRB1). Using metagenomic sequencing and 34S-enriched sulfate stable isotope probing coupled with nanoSIMS, we demonstrate interspecies transfer of reduced sulfur metabolites from PB-SRB1 to PB-PSB1. The pink berries catalyse net sulfide oxidation and maintain internal sulfide concentrations of 0–500 μm. Sulfide within the berries, captured on silver wires and analysed using secondary ion mass spectrometer, increased in abundance towards the berry interior, while δ34S-sulfide decreased from 6‰ to −31‰ from the exterior to interior of the berry. These values correspond to sulfate–sulfide isotopic fractionations (15–53‰) consistent with either sulfate reduction or a mixture of reductive and oxidative metabolisms. Together this combined metagenomic and high-resolution isotopic analysis demonstrates active sulfur cycling at the microscale within well-structured macroscopic consortia consisting of sulfide-oxidizing anoxygenic phototrophs and sulfate-reducing bacteria.

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Thiohalocapsa marina sp. nov., from an Indian marine aquaculture pond

Cited by 14 publications

References 18 publications

A Nanoscale Study of Carbon and Nitrogen Fluxes in Mats of Purple Sulfur Bacteria: Implications for Carbon Cycling at the Surface of Coastal Sediments

A Nanoscale Study of Carbon and Nitrogen Fluxes in Mats of Purple Sulfur Bacteria: Implications for Carbon Cycling at the Surface of Coastal Sediments

Microscale sulfur cycling in the phototrophic pink berry consortia of the Sippewissett Salt Marsh

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