Thiol-disulfide homeostasis and ischemia-modified albumin levels as indicators of oxidative stress in welders’ lung disease

Abstract

<jats:p> Welders’ lung disease refers to mixed exposure to different kinds of metals and chemicals from welding fumes, which affect all parts of the respiratory tract including airways and parenchyma together. This study aimed to investigate the oxidative status in patients with welders’ lung (PWL) by means of thiol-disulfide homeostasis and ischemia-modified albumin (IMA) levels. The male welder workers diagnosed with welders’ lung disease and healthy individuals were recruited in the study. Plasma levels of disulfide, disulfide/native thiol ratio, disulfide/total thiol ratio, IMA, and catalase (CAT) were determined. Pulmonary function test parameters of both groups were compared. The thiol-disulfide homeostasis parameters of PWL and control group were as follows: disulfide (20.5 ± 6.3 vs. 16.2 ± 3.9 μmol L<jats:sup>−1</jats:sup>, p &lt; 0.001), disulfide/native thiol (4.36 (1.59) vs. 4.0 (1.64), p = 0.024), and disulfide/total thiol (4.01 (1.34) vs. 3.71 (1.41), p = 0.024). IMA levels in PWL were significantly higher than the control group (1.37 (0.27) mg dL<jats:sup>−1</jats:sup> vs. 0.49 (0.61) mg dL<jats:sup>−1</jats:sup>, p &lt; 0.001), whereas CAT activities were significantly higher in the control group (106.6 (54.5) kU L<jats:sup>−1</jats:sup> vs. 78.3 (67.8) kU L<jats:sup>−1</jats:sup>, p = 0.003). The findings of the present study revealed that oxidative stress plays a key role in the pathogenesis of welders’ lung disease. Plasma thiol-disulfide homeostasis and IMA levels might be indicators of oxidative stress in PWL. </jats:p>

Pathological changes of lung tissue and oxidative stress in rats exposed to welding fumes

“…They also found a signi cant decrease in the activity of catalase (CAT) in workers with lung disease compared to controls. These results indicate the importance of oxidative stress in the pathogenesis of Welder's lung disease (17). Graczyk et al (2016) carried outa human study on the welding fume impacts on 20 non-smoking workers.…”

Relationship of thiol/disulphide homeostasis with oxidative stress parameters in non-diabetic, prediabetic and type 2 diabetic Turkish women

“…Under OS conditions, the thiol groups of the aminoacids with sulphur groups such as cysteine and methionine form reversible disulphide bonds with the low molecular weight thiol groups. This is known as dynamic TDH and has been investigated in several diseases such as Graves' disease (Agan et al, 2019), childhood iron deficiency anemia (Topal, et al, 2019), Welders' lung disease (Karatas et al, 2019) stage were found to be significantly lower than the lower stage patients. In contrast, ARES levels were found to correlate with nuclear grade in renal cell carcinoma patients (Aldemir et al, 2015).…”

The effect of two formulations of carbon enterosorbents on oxidative stress indexes and molecular conformation of serum albumin in experimental animals exposed to CCl4

“…An intensity of this process is predetermined by amino acid composition of proteins and could be considered as an early marker of oxidative stress. The degree of oxidative modification of albumin can be estimated by the concentration of mercaptalbumin and non-mercaptalbumin or their ratio, or by determination of albumin ability to bind cobalt iones (Albumin Cobalt Binding Test) [46,47]. However, both of these methods do not give the information about the conformational changes in this protein in response to the oxidation process.…”