Thiol-Disulphide Homeostasis in Ovarian Torsion-Detorsion: An Experimental Rat Model

Abstract

Ovarian torsion is one of the reasons of acute abdomen requiring immediate surgical intervention. There is not any reliable method for its diagnosis. Delay in treatment may result in loss of ovary. In this study, the purpose is to evaluate efficiency of thioldisulphide homeostasis in early diagnosis of ovarian torsion in experimental rat model and in its follow-up care with biochemical and histopathological findings. Material and Methods: In the study, 24 Albino Wistar female rats were divided into three groups. The first group was the sham group. The second group was the torsion group. The last group was the torsion-detorsion group. Histopathological evaluation was carried out in ovaries in all groups, and values of thiol/disulphide homeostasis were measured in serum samples biochemically. Results: Values of native thiol and total thiol were lower in the torsion-detorsion group compared to the other groups. However, there was no significant difference between of native thiol and total thiol levels in the sham and torsion groups. Interestingly, the index of native thiol/total thiol was relatively low in the torsion and torsion-detorsion groups compared to the sham group. However, this decrease was not significant. Conclusion: These results suggest that native thiol/total thiol values may not be useful for early diagnosis of ovarian torsion but it may be used for follow-up care after detorsion

Comparison of Pre-Operative and Post-Operative Thiol-Disulfide Levels in Acute Abdomen Patients

“…These parameters were investigated in the diagnosis of many causes of acute abdomen. [7][8][9] In addition to inflammatory markers, oxidative stress markers have recently become focus of interest in research efforts. In the literature, there is limited number of experimental and clinical trials addressing relationship between acute abdomen and oxidative stress.…”