Thiolated pectin–doxorubicin conjugates: Synthesis, characterization and anticancer activity studies

Cheewatanakornkool, Kamonrak; Niratisai, Sathit; Manchun, Somkamol; Dass, Crispin R.; Sriamornsak, Pornsak

doi:10.1016/j.carbpol.2017.06.115

Cited by 40 publications

(15 citation statements)

References 32 publications

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“…The content of free thiol groups in conjugate was determined by Ellman's reagent method. 41 Briefly, 40 mg of DTNB was dissolved in 10 mL of 0.1 M PBS (pH 8.0, including 1 mM EDTA) to prepare Ellman's reagent. Then 20 mg of fresh Thio-alg-1, Thio-alg-2, and Thio-alg-3 conjugates was, respectively, dispersed in 10 mL of PBS to prepare sample solutions.…”

Section: Methodsmentioning

confidence: 99%

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Development of Microspheres Based on Thiol-Modified Sodium Alginate for Intestinal-Targeted Drug Delivery

Mao

Zhang

et al. 2019

ACS Appl. Bio Mater.

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Bioactive peptide drugs are mostly delivered by parenteral administration, which brings great pain and risks to patients. Oral administration is an acceptable alternative form. However, peptide drugs are extremely sensitive to the strong acidic environment in the stomach after oral administration. They would be degraded by pepsin and trypsin in the gastrointestinal tract. Herein, we present microspheres for intestinal-targeted peptides drug delivery through oral administration. Sodium alginate was reacted with l-cysteine to bring it into thiol groups. Then sodium alginate-l-cysteine conjugates were mixed with native sodium alginate and emulsified by an improved method. Ca2+ was used to fix the emulsion to get the microspheres. Bovine serum albumin was used as the simulating drug to assess the feasibility of microspheres as intestinal delivery carriers. The results showed that the microspheres exhibited spherical properties and narrow size distribution. The drug-loading capacity of microspheres was not compromised after thiol-modification. It is interesting that the microspheres can maintain structural integrity and hold drugs in the strong acidic environment in the stomach. Conversely, the microspheres presented sustained intestinal-targeted drugs release ability as expected. Moreover, thiol-modification further improved the adherence ability of microspheres on the inner walls of the small intestine, which is good for enhancing drug permeability. In sum, the microspheres based on thiol-modified sodium alginate have promising applications as intestinal-targeted macromolecular drug carriers.

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Section: Methodsmentioning

confidence: 99%

“…The content of free thiol groups in conjugate was determined by Ellman’s reagent method . Briefly, 40 mg of DTNB was dissolved in 10 mL of 0.1 M PBS (pH 8.0, including 1 mM EDTA) to prepare Ellman’s reagent.…”

Section: Methodsmentioning

confidence: 99%

Development of Microspheres Based on Thiol-Modified Sodium Alginate for Intestinal-Targeted Drug Delivery

Mao

Zhang

et al. 2019

ACS Appl. Bio Mater.

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“…The thiolate pectin–DOX conjugate showed more anti-cancer activity than free DOX in the case of 143B and CT26 cells, but no significant difference was seen between the free DOX and thiolate pectin-DOX in the case of the prostate cancer cells. Thiolated pectin–DOX conjugates have been used in targeted drug delivery in CT26 cells [ 102 ]. Self-assembled DOX-conjugated hydrophilic pectin nanoparticles loaded with hydrophobic dihydroartemisinin revealed a quick release of DOX and DHA in a weakly acidic environment.…”

Section: Polysaccharides Based Drug Carriersmentioning

confidence: 99%

Polysaccharide-Drug Conjugates: A Tool for Enhanced Cancer Therapy

et al. 2022

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Cancer is one of the most widespread deadly diseases, following cardiovascular disease, worldwide. Chemotherapy is widely used in combination with surgery, hormone and radiation therapy to treat various cancers. However, chemotherapeutic drugs can cause severe side effects due to non-specific targeting, poor bioavailability, low therapeutic indices, and high dose requirements. Several drug carriers successfully overcome these issues and deliver drugs to the desired sites, reducing the side effects. Among various drug delivery systems, polysaccharide-based carriers that target only the cancer cells have been developed to overcome the toxicity of chemotherapeutics. Polysaccharides are non-toxic, biodegradable, hydrophilic biopolymers that can be easily modified chemically to improve the bioavailability and stability for delivering therapeutics into cancer tissues. Different polysaccharides, such as chitosan, alginates, cyclodextrin, pullulan, hyaluronic acid, dextran, guar gum, pectin, and cellulose, have been used in anti-cancer drug delivery systems. This review highlights the recent progress made in polysaccharides-based drug carriers in anti-cancer therapy.

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“…Thiomers are a class of mucoadhesive materials which can be synthesised through the immobilisation of thiol groups on various materials. Some examples of thiomers include thiolated-polycarbophil [73], chitosan [74], pectin [75,76], graphene oxide [77], polyvinylpyrrolidone [78], and hyaluronic acid [79]. Their mucoadhesive properties can be due to their ability to form disulfide bonds with cysteine-rich domains of mucus glycoproteins via thiol/disulfide exchange reactions or oxidation of their thiol groups.…”

Section: Transmucosal Drug Deliverymentioning

confidence: 99%

Silica Nanoparticles in Transmucosal Drug Delivery

Ways

Lau

et al. 2020

Pharmaceutics

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Transmucosal drug delivery includes the administration of drugs via various mucous membranes, such as gastrointestinal, nasal, ocular, and vaginal mucosa. The use of nanoparticles in transmucosal drug delivery has several advantages, including the protection of drugs against the harsh environment of the mucosal lumens and surfaces, increased drug residence time, and enhanced drug absorption. Due to their relatively simple synthetic methods for preparation, safety profile, and possibilities of surface functionalisation, silica nanoparticles are highly promising for transmucosal drug delivery. This review provides a description of silica nanoparticles and outlines the preparation methods for various core and surface-functionalised silica nanoparticles. The relationship between the functionalities of silica nanoparticles and their interactions with various mucous membranes are critically analysed. Applications of silica nanoparticles in transmucosal drug delivery are also discussed.

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Thiolated pectin–doxorubicin conjugates: Synthesis, characterization and anticancer activity studies

Cited by 40 publications

References 32 publications

Development of Microspheres Based on Thiol-Modified Sodium Alginate for Intestinal-Targeted Drug Delivery

Development of Microspheres Based on Thiol-Modified Sodium Alginate for Intestinal-Targeted Drug Delivery

Polysaccharide-Drug Conjugates: A Tool for Enhanced Cancer Therapy

Silica Nanoparticles in Transmucosal Drug Delivery

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