Thiomicrorhabdus indica sp. nov., an obligately chemolithoautotrophic, sulfur-oxidizing bacterium isolated from a deep-sea hydrothermal vent environment

Li, Xuewen; Jiang, Lijing; Hu, Qitao; Lyu, Jie; Shao, Zongze

doi:10.1099/ijsem.0.003744

Cited by 19 publications

(22 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several sulfur-oxidizing bacteria (SOB), belonging to the class Gammaproteobacteria, have also been isolated from hydrothermal habitats (Table 1, Supplementary Table S1). The main sulfur-oxidizing gammaproteobacterial taxa are in the order Thiotrichales, including Hydrogenovibrio crunogenus (formerly Thiomicrospira crunogena) (Jannasch et al 1985), Hydrogenovibrio thermophile strain I78 (formerly T. thermophile) (Takai et al 2004d), H. thermophilus strain S5 (Jiang et al 2017), Thiomicrorhabdus indica (Liu et al 2020); Thiomicrorhabdus sp. Kp2 is mentioned in the reclassification of these genera (Boden et al 2017b).…”

Section: Proteobacteriamentioning

confidence: 99%

Microorganisms from deep-sea hydrothermal vents

Zeng

Alain

Shao

2021

Mar Life Sci Technol

Self Cite

View full text Add to dashboard Cite

With a rich variety of chemical energy sources and steep physical and chemical gradients, hydrothermal vent systems offer a range of habitats to support microbial life. Cultivation-dependent and independent studies have led to an emerging view that diverse microorganisms in deep-sea hydrothermal vents live their chemolithoautotrophic, heterotrophic, or mixotrophic life with versatile metabolic strategies. Biogeochemical processes are mediated by microorganisms, and notably, processes involving or coupling the carbon, sulfur, hydrogen, nitrogen, and metal cycles in these unique ecosystems. Here, we review the taxonomic and physiological diversity of microbial prokaryotic life from cosmopolitan to endemic taxa and emphasize their significant roles in the biogeochemical processes in deep-sea hydrothermal vents. According to the physiology of the targeted taxa and their needs inferred from meta-omics data, the media for selective cultivation can be designed with a wide range of physicochemical conditions such as temperature, pH, hydrostatic pressure, electron donors and acceptors, carbon sources, nitrogen sources, and growth factors. The application of novel cultivation techniques with real-time monitoring of microbial diversity and metabolic substrates and products are also recommended.

show abstract

Section: Proteobacteriamentioning

confidence: 99%

Microorganisms from deep-sea hydrothermal vents

Zeng

Alain

Shao

2021

Mar Life Sci Technol

Self Cite

View full text Add to dashboard Cite

show abstract

“…ezbiocloud. net) [14] showed that strain G1 T was closely related to Thiomicrorhabdus hydrogeniphila MAS2 T [3,8] (96.0 % sequence identity) and that strain G2 T was closely related to Thiomicrorhabdus indica 13-15A T [5] (95.4 % sequence identity). Meanwhile, the 16S rRNA gene sequence identity between strains G1 T and G2 T was 95.8 %, suggesting that strains G1 T and G2 T may represent two different novel species within the genus Thiomicrorhabdus.…”

Section: S Rrna Gene Phylogenymentioning

confidence: 99%

“… Thiomicrorhabdus is widespread in the aquatic environment especially marine environments including sediment, seawater, deep-sea hydrothermal vents and so on [2–7]. At the time of writing, the genus Thiomicrorhabdus contains seven species with validly published names: Thiomicrorhabdus frisia [1, 2], Thiomicrorhabdus arctica [1, 7], Thiomicrorhabdus psychrophila [1, 7], Thiomicrorhabdus chilensis [6], Thiomicrorhabdus hydrogeniphila [3, 8], Thiomicrorhabdu indica [5] and Thiomicrorhabdus aquaedulcis [4]. These species are characterized as Gram-stain-negative, rod-shaped, and capable of growing chemolithoautotrophically with reduced sulphur compounds, such as sulfide, thiosulfate and elemental sulphur [1].…”

Section: Full-textmentioning

confidence: 99%

Thiomicrorhabdus sediminis sp. nov. and Thiomicrorhabdus xiamenensis sp. nov., novel sulfur-oxidizing bacteria isolated from coastal sediments and an emended description of the genus Thiomicrorhabdus

Chen

Lai

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

Self Cite

View full text Add to dashboard Cite

Two novel Gram-strain-negative and rod-shaped bacteria, designated strain G1T and G2T, were isolated from sediment samples collected from the coast of Xiamen, PR China. The cells were motile by a single polar flagellum. Growth of strain G1T occurred at 10–40 °C (optimum, 30 °C), at pH 6.0–9.0 (optimum, pH 7.5) and with 5–1530 mM NaCl (optimum, 510 mM), while the temperature, pH and NaCl concentration ranges for G2T were 4–45 °C (optimum, 28 °C), pH 5.5–8.0 (optimum, pH 6.5) and 85–1530 mM NaCl (optimum, 340 mM). The two isolates were obligate chemolithoautotrophs capable of using thiosulfate, sulfide, elemental sulphur or tetrathionate as an energy source. Strain G1T used molecular oxygen or nitrite as an electron acceptor, while strain G2T used molecular oxygen as the sole electron acceptor. The dominant fatty acids of G1T and G2T were summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C16 : 0 and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). The DNA G+C content of G1T and G2T were 45.1 and 48.3 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain G1T and G2T were members of the genus Thiomicrorhabdus , and most closely related to Thiomicrorhabdus hydrogeniphila MAS2T (96.0 %) and Thiomicrorhabdus indica 13-15AT (95.4 %), respectively. The 16S rRNA gene sequence similarity between strains G1T and G2T was 95.8 %. Based on the phylogenetic, genomic and phenotypic data presented here, the isolate strains represent novel species of the genus Thiomicrorhabdus , for which the names Thiomicrorhabdus sediminis sp. nov. (type strain G1T=MCCC 1A14511T=KCTC 15841T) and Thiomicrorhabdus xiamenensis sp. nov. (type strain G2T=MCCC 1A14512T=KCTC 15842T) are proposed.

show abstract

“…The reclassified five species are T. frisia (Brinkhoff et al, 1999a), T. chilensis (Brinkhoff et al, 1999b), T. arctica, T. psychrophila (Knittel et al, 2005) and T. hydrogeniphila (Watsuji et al, 2016). The other four species described in this genus are T. aquaedulcis (Kojima & Fukui 2019), T. indica (Liu et al, 2020), T. sediminis and T. xiamenensis (Liu et al, 2021). They are all obligate chemolithoautotrophs which oxidize sulfur compounds.…”

Section: Introductionmentioning

confidence: 99%

“…T (Watsuji et al, 2016); 7, T. aquadulcis HaS4 T(Kojima & Fukui, 2019); 8, T. indica 13-15A T(Liu et al, 2020); 9, T. sediminis G1 T(Liu et al, 2021); 10, T. xiamenensis G2 T(Liu et al, 2021). All strains are obligate chemolithoautotrophs which oxidize thiosulfate, tetrathionate and sulfur.…”

mentioning

confidence: 99%

Thiomicrorhabdus immobilis sp. nov., a mesophilic sulfuroxidizing bacterium isolated from sediment of a brackish lake

Kojima

Mochizuki

Kanda

et al. 2022

Preprint

View full text Add to dashboard Cite

A novel sulfur-oxidizing bacterium, strain Am19T , was isolated from sediment of a brackish lake. Strain Am19T grew chemolithotrophically on inorganic sulfur compounds, and heterotrophic growth was not observed. Cells were rod-shaped with length of 1.1–3.0 μm and width of 0.5–0.8 μm. Growth was observed at 5–37°C with an optimum growth temperature of 30°C. The pH range for growth was 5.6–8.5 with an optimum pH of 6.6–7.0. Major fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), summed feature 8 (C18 : 1w7c and/or C18 : 1w6c) and C16 : 0. The sole respiratory quinone was ubiquinone-8. The complete genome of strain Am19T is composed of a circular chromosome with length of 2.5 Mbp and G + C content of 42.7 mol%. Phylogenetic analysis based on genomic data indicated that strain Am19 T belongs to the genus Thiomicrorhabdus but is distinct from any existing species. Analysis of the 16S rRNA gene supported creation of a new species to accommodate strain Am19T . On the basis of genomic and phenotypic characteristics, strain Am19T (= NBRC 114602T = BCRC 81336T ) is proposed as the type strain of a new species, with name of Thiomicrorhabdus immobilis sp. nov.

show abstract

Thiomicrorhabdus indica sp. nov., an obligately chemolithoautotrophic, sulfur-oxidizing bacterium isolated from a deep-sea hydrothermal vent environment

Cited by 19 publications

References 18 publications

Microorganisms from deep-sea hydrothermal vents

Microorganisms from deep-sea hydrothermal vents

Thiomicrorhabdus sediminis sp. nov. and Thiomicrorhabdus xiamenensis sp. nov., novel sulfur-oxidizing bacteria isolated from coastal sediments and an emended description of the genus Thiomicrorhabdus

Thiomicrorhabdus immobilis sp. nov., a mesophilic sulfuroxidizing bacterium isolated from sediment of a brackish lake

Contact Info

Product

Resources

About