Thiophanate-methyl induces severe hepatotoxicity in zebrafish

Jia, Kun; Cheng, Bo; Huang, Lirong; Xiao, Jianghong; Bai, Zhonghui; Liao, Xinjun; Cao, Zigang; Shen, Tianzhu; Zhang, Chunping; Hu, Chengyu; Lu, Huiqiang

doi:10.1016/j.chemosphere.2020.125941

Cited by 69 publications

(25 citation statements)

References 43 publications

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“…We here acknowledge that this may constitute a limitation of interpreting immunohistochemistry data since we cannot rule out the remote possibility of crossreactivity with the pro-caspase 3 (37 kDa). Thus, supporting the validity of our findings, other studies have previously demonstrated the specificity of this antibody for the active form of zebrafish CAS3 (44)(45)(46)(47)(48).…”

Section: Discussionsupporting

confidence: 91%

Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary

et al. 2021

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Di-isononyl phthalate (DiNP) is a plasticizer reported to elicit hormone-like activity and disrupt metabolism and reproduction in fish and other vertebrates. In general, phthalates have been used at high concentrations beyond reported environmental levels to assess their adverse effects on fish gonadal physiology. The present study exposed adult female zebrafish to a wide range of DiNP concentrations [0.42 µg L−1 (10−9 M), 4.2 µg L−1 (10−8 M), and 42 µg L−1 (10−7 M)] for 21 days. We evaluated gene expression profiles related to apoptosis, autophagy, and oxidative stress; DNA fragmentation (TUNEL assay: terminal deoxynucleotidyl transferase dUTP nick end labeling) and caspase activity (CAS3) were also examined. Exposure to 0.42 and 4.2 µg L−1 upregulated the genes coding for tnfa and baxa, sod1, prkaa1, respectively. CAS3 immunohistochemistry revealed a higher number of positive vitellogenic oocytes in ovaries exposed to 0.42 µg L−1. Subsequently, we examined the relationship between CAS3 signaling and DNA fragmentation. Accordingly, DNA fragmentation was observed in vitellogenic follicles of fish exposed to 0.42 and 4.2 μg L−1. Our results demonstrate that follicular atresia can occur after exposure to environmental levels of DiNP for 21 days, which may adversely affect the reproductive performance of female zebrafish in a non-monotonic manner.

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Section: Discussionsupporting

confidence: 91%

Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary

et al. 2021

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“…Zebrafish (Danio rerio) has emerged as an advantageous in vivo vertebrate model organism for mechanistic interpreting and high-throughput candidate drug screenings. Zebrafish offers a combination of rapid development, high fecundity, high-throughput screening, and significant morphological and physiological homology to mammals in spite of the reasonable cost [ 33 , 34 ]. Specially, zebrafish completes primary liver morphogenesis in 48 h postfertilization (hpf) and full liver function in 72 hpf.…”

Section: Resultsmentioning

confidence: 99%

Hepatoprotection of Lycii Fructus Polysaccharide against Oxidative Stress in Hepatocytes and Larval Zebrafish

Zhang

et al. 2021

Oxidative Medicine and Cellular Longevity

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Scavenging of oxidative stress by antioxidants may provide a therapeutic strategy for nonalcoholic fatty liver disease (NAFLD). Increasing evidence is supporting the potential application of natural resourced polysaccharides as promising prevention or treatment strategies against NAFLD. In the current study, an acidic heteropolysaccharide, LFP-a1, was isolated and purified from Lycii fructus with successively hot water refluxing extraction, alcohol precipitation, protein removal, and DEAE-52 cellulose chromatographic separation. LFP-a1 was a complicated structured polysaccharide with an average MW of 4.74 × 104 Da and composed of 6 monosaccharides and 1 uronic acid. Preexposure of LFP-a1 could increase the cell viability and reverse the abnormal oxidative stress though inhibition of mitochondrial-mediated apoptotic pathway and correction of cell cycle progression against H2O2 hepatoxicity in NAFLD model L02 cells. Consistently, in vivo study in thioacetamide- (TAA-) induced NAFLD model zebrafish larvae showed LFP-a1 preserved the liver integrity and alleviated TAA-induced oxidative stress through downregulation of abnormal apoptosis. These observations indicated the hepatoprotective activity of LFP-a1, which may be applied for the prevention or treatment of NAFLD or other oxidative stress-related diseases.

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“…Each experiment was repeated three times. The methods of Jia et al 38 and He et al 39 were adopted. Proliferating cell nuclear antigen (PCNA) (Abcam, Cas no.…”

Section: Methodsmentioning

confidence: 99%

Toxicity of thioacetamide and protective effects of quercetin in zebrafish (Danio rerio) larvae

Wang

Deng

Zhang

et al. 2021

Environmental Toxicology

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Quercetin is a flavonoid compound with a variety of biological properties that is widely distributed throughout the plant kingdom. Studies have found that quercetin has anti‐inflammatory, antioxidant, and liver‐protective effects, while thioacetamide (TAA) can cause inflammation and liver damage in zebrafish larvae. The purpose of this study was to evaluate whether quercetin can prevent TAA‐induced inflammation and liver damage in zebrafish larvae and to investigate the molecular mechanisms involved. Zebrafish Tg transgenic lines were used as the experimental animals. Behavioral, oxidative stress level, proliferative antigen chromogenic antibody, and western blot analyses were carried out on zebrafish larvae in the control group and groups treated with TAA and 12 μM quercetin. The results indicated that quercetin promoted the development of zebrafish larvae damaged by TAA, exhibited antioxidant and anti‐inflammatory properties, and promoted cell proliferation. Quercetin reduced the expression of p53 protein in zebrafish larvae injured by TAA, resulting in decreased levels of Bax and increased levels of Bcl‐2. The findings suggested quercetin has antiapoptotic action. Quercetin reduced the expression of DKK1 and DKK2 genes related to the Wnt signaling pathway in zebrafish larvae damaged by TAA and increased the expression of Lef1 and wnt2bb. Quercetin may regulate the development of zebrafish larvae damaged by TAA through the Wnt signaling pathway. This study provides the scientific basis for the development and utilization of quercetin and the development of new related drugs.

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Thiophanate-methyl induces severe hepatotoxicity in zebrafish

Cited by 69 publications

References 43 publications

Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary

Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary

Hepatoprotection of Lycii Fructus Polysaccharide against Oxidative Stress in Hepatocytes and Larval Zebrafish

Toxicity of thioacetamide and protective effects of quercetin in zebrafish (Danio rerio) larvae

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