Thioredoxin reductase is a key factor in the oxidative stress response of Lactobacillus plantarum WCFS1

Serrano, L.M.; Molenaar, Douwe; Wels, Michiel; Teusink, Bas; Bron, Peter A.; Vos, Willem M. de; Smid, Eddy J.

doi:10.1186/1475-2859-6-29

Cited by 113 publications

(75 citation statements)

References 37 publications

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“…Four homologous genes were upregulated in the luxS mutant, perhaps resulting in downregulation of 102 prophage genes (see Table S2 in the supplemental material). This type of stress regulation of prophage genes has been reported in Lactobacillus plantarum and B. subtilis (12,14). We propose that the accumulation of methionine in mutant cells reflects the derangement of the AMC.…”

Section: Resultsmentioning

confidence: 91%

Transcriptional and Metabolomic Consequences of luxS Inactivation Reveal a Metabolic Rather than Quorum-Sensing Role for LuxS in Lactobacillus reuteri 100-23

et al. 2012

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c Autoinducer-2 (AI-2)-mediated quorum sensing has been extensively studied in relation to the regulation of microbial behavior. There are, however, two potential roles for the AI-2 synthase (LuxS). The first is in the production of AI-2 and the second is as an enzyme in the activated methyl cycle, where it catalyzes the conversion of S-ribosylhomocysteine to homocysteine. The by-product of the reaction catalyzed by LuxS is (S)-4,5-dihydroxy-2,3-pentanedione, which spontaneously forms the furanones known collectively as AI-2. The mammalian gut contains a complex collection of bacterial species so a method of interspecies communication might influence community structure and function. Lactobacillus reuteri 100-23 is an autochthonous inhabitant of the rodent forestomach, where it adheres to the nonsecretory epithelium, forming a biofilm. Microarray comparisons of gene expression profiles of the L. reuteri 100-23 wild type and a luxS mutant under different culture conditions revealed altered transcription of genes encoding proteins associated with cysteine biosynthesis/oxidative stress response, urease activity, and sortasedependent proteins. Metabolomic analysis showed that the luxS mutation affected cellular levels of fermentation products, fatty acids and amino acids. Cell density-dependent changes (log phase versus stationary phase growth) in gene transcription were not detected, indicating that AI-2 was unlikely to be involved in gene regulation mediated by quorum sensing in L. reuteri 100-23.

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Section: Resultsmentioning

confidence: 91%

Transcriptional and Metabolomic Consequences of luxS Inactivation Reveal a Metabolic Rather than Quorum-Sensing Role for LuxS in Lactobacillus reuteri 100-23

et al. 2012

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“…Protection against oxidative stress in LAB involves a variety of functions that are highly variable among the LAB species and strains (including many oxidases, peroxidases, catalases, and superoxide dismutases [see above]). Nevertheless, a central role in oxidative stress tolerance has been proposed for the strongly conserved thioredoxin system (TrxA and TrxB) (588,590,591). Many environmental stress conditions can lead to DNA damage, and DNA damage responses also appear to be strongly conserved among LAB, including functions associated with homologous recombination and double-strand-break repair (Rec-ABDFJNOR, RuvAB, and Ssb), the homology-independent facilitator complex (GyrAB and TopA), and the pathway involved in base excision repair (Mfd, UvrABCD, and Xth), although the endonuclease IV (Nfo) that is important in this pathway is absent in many species (588).…”

Section: Comparative Genomics Of the Lab Stressomementioning

confidence: 99%

Stress Physiology of Lactic Acid Bacteria

Papadimitriou

Alegría

Bron

et al. 2016

Microbiol Mol Biol Rev

540

404

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SUMMARYLactic acid bacteria (LAB) are important starter, commensal, or pathogenic microorganisms. The stress physiology of LAB has been studied in depth for over 2 decades, fueled mostly by the technological implications of LAB robustness in the food industry. Survival of probiotic LAB in the host and the potential relatedness of LAB virulence to their stress resilience have intensified interest in the field. Thus, a wealth of information concerning stress responses exists today for strains as diverse as starter (e.g.,Lactococcus lactis), probiotic (e.g., severalLactobacillusspp.), and pathogenic (e.g.,EnterococcusandStreptococcusspp.) LAB. Here we present the state of the art for LAB stress behavior. We describe the multitude of stresses that LAB are confronted with, and we present the experimental context used to study the stress responses of LAB, focusing on adaptation, habituation, and cross-protection as well as on self-induced multistress resistance in stationary phase, biofilms, and dormancy. We also consider stress responses at the population and single-cell levels. Subsequently, we concentrate on the stress defense mechanisms that have been reported to date, grouping them according to their direct participation in preserving cell energy, defending macromolecules, and protecting the cell envelope. Stress-induced responses of probiotic LAB and commensal/pathogenic LAB are highlighted separately due to the complexity of the peculiar multistress conditions to which these bacteria are subjected in their hosts. Induction of prophages under environmental stresses is then discussed. Finally, we present systems-based strategies to characterize the “stressome” of LAB and to engineer new food-related and probiotic LAB with improved stress tolerance.

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“…The survival of L. plantarum under harsh and diverse conditions indicates that it has developed several tolerance and resistance mechanisms (20,28). Although some stress genes have been characterized in this species (9,27,29,30), little is known about their transcriptional regulation.…”

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confidence: 99%

Characterization of the CtsR Stress Response Regulon in Lactobacillus plantarum

et al. 2010

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Lactobacillus plantarum ctsR was characterized. ctsR was found to be cotranscribed with clpC and induced in response to various abiotic stresses. ctsR deletion conferred a heat-sensitive phenotype with peculiar cell morphological features. The transcriptional pattern of putative CtsR regulon genes was examined in the ⌬ctsR mutant. Direct CtsR-dependent regulation was demonstrated by DNA-binding assays using recombinant CtsR and the promoters of the ctsR-clpC operon and hsp1.

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Thioredoxin reductase is a key factor in the oxidative stress response of Lactobacillus plantarum WCFS1

Abstract: Background: Thioredoxin (TRX) is a powerful disulfide oxido-reductase that catalyzes a wide spectrum of redox reactions in the cell. The aim of this study is to elucidate the role of the TRX system in the oxidative stress response in Lactobacillus plantarum WCFS1.

Cited by 113 publications

References 37 publications

Transcriptional and Metabolomic Consequences of luxS Inactivation Reveal a Metabolic Rather than Quorum-Sensing Role for LuxS in Lactobacillus reuteri 100-23

Transcriptional and Metabolomic Consequences of luxS Inactivation Reveal a Metabolic Rather than Quorum-Sensing Role for LuxS in Lactobacillus reuteri 100-23

Stress Physiology of Lactic Acid Bacteria

Characterization of the CtsR Stress Response Regulon in Lactobacillus plantarum

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