Thiourea modulates the expression and activity profile of mtATPase under salinity stress in seeds of Brassica juncea

Srivastava, Ashish Kumar; Ramaswamy, N.K.; Mukopadhyaya, R.; Jincy, M.; D’Souza, S.F.

doi:10.1093/aob/mcn229

Cited by 55 publications

(45 citation statements)

References 41 publications

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“…The use of TU as a stress ameliorating agent has been successfully demonstrated both at the field level (Sahu et al 2005) as well as under other abiotic stresses at the laboratory level (Srivastava et al 2009(Srivastava et al , 2010b. The preliminary analysis of seedling phenotype was performed to evaluate the differential growth pattern of the seedlings in response to TU supplementation under UV-B stress.…”

Section: Discussionmentioning

confidence: 99%

“…Among the three compounds, the performance of TU was found to be superior (Sahu et al 2005). Considering the efficiency of TU in increasing the crop yield under stress conditions and the positive role of TU treatment in alleviating salinity stress effects (Srivastava et al 2009(Srivastava et al , 2010a, it was thought worthwhile to undertake studies to investigate the potential of TU in alleviating the abiotic stresses including UV-B in Brassica juncea. In the present study, comparative biochemical and molecular responses of UV-B versus UV-B'TU were studied using the Indian mustard.…”

Section: Introductionmentioning

confidence: 99%

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Thiourea mediates alleviation of UV-B stress-induced damage in the Indian mustard (Brassica junceaL.)

Pandey

Srivastava

Suprasanna

et al. 2012

Journal of Plant Interactions

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UV-B radiation (280Á320 nm) as an environmental stress has damaging effect on plants and its overexposure can potentially interfere with growth and development. The effect of thiourea (TU) on UV-B stress tolerance of 10-day-old Brassica juncea seedlings subjected to supplementary UV-B for 5 days was investigated. An increase in the UV-B absorbing compounds anthocyanin, flavonoids and phenolic compounds was observed in UV-B'TU treated seedlings as compared to that of control. The enhanced synthesis of UV-B screening compounds resulted in lesser damage to chlorophyll and also gain in fresh weight and dry weight in UV-B'TU as compared to UV-B alone treatment. The enzymatic activities of guaiacol peroxidase (GPX) and superoxide dismutase (SOD) also increased in UV-B'TU. The expression profiling of phenylalanine ammonia lyase (pal) and chalcone synthase (chs) indicated an upregulation under UV-B'TU treatment, compared to that of control, suggesting that TU treatment ensured an early and efficient induction of flavonoid biosynthetic pathway. The results indicate that TU helps in ameliorating the damaging effects of UV-B stress by efficiently maintaining the antioxidant status and attenuating the penetration of the UV-B.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Thiourea mediates alleviation of UV-B stress-induced damage in the Indian mustard (Brassica junceaL.)

Pandey

Srivastava

Suprasanna

et al. 2012

Journal of Plant Interactions

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“…Because ATP energy is very essential for processes such as Na + efflux by Na + -ATP synthase, Na + -K + /H + transport across the plasma membrane, and control of K + uptake and Na + flow by vacuolar V-ATP synthase (Srivastava et al, 2009;Soontharapirakkul et al, 2011), ATP is the current rate of energy in the cell and this ATP is provided chiefly by mitochondrial F 1 F 0 -ATP synthase (Srivastava et al, 2009;Soontharapirakkul et al, 2011).…”

Section: Several Hormone-and Environment-related Cis-elements Were Prmentioning

confidence: 99%

Isolation and in silico functional analysis of MtATP6, a 6-kDa subunit of mitochondrial F1F0-ATP synthase, in response to abiotic stress

Moghadam

Taghavi²,

Niazi‎³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Mitochondrial F 1 F 0 -ATP synthase is a key enzymatic complex of energy metabolism that provides ATP for the cell. Subunits of this enzyme over-express under stress conditions. Little is known about the structure and regulatory mechanism of the F 0 portion of this enzyme. We isolated the full-length coding sequence of the RMtATP6 gene from rice and wheat, and partial sequences from Aegilops crassa and Triticum monococcum (Poaceae). We found that the sequence of rice RMtATP6 is 1965 bp long and contains two exons and one intron in 3'-UTR. Then, we analyzed the 2000-bp upstream region of the initiation codon ATG of the RMtATP6 and AtMtATP6, as promoter. The RMtATP6 coding sequence was found to be much conserved in the different plant species, possibly because of its key role under stress conditions. Promoter analysis demonstrated that RMtATP6 and AtMtATP6 include cis-acting elements such as ABRE, MYC/MYB, GT element in the upstream region, which respond to abscisic acid stress hormone and might show vital its roles in biotic and abiotic tolerance as an early-stress responsive gene. A mitochondrial signal peptide of 30 amino acids in length and an N-terminal cleavage site between amino acids 20 and 21 were discovered in RMtATP6. In addition, we found a transmembrane domain with an alpha helix structure that possibly passed through the mitochondrial inner membrane and established the 6-kDa subunit in the F 0 portion of the enzyme complex. Apparently, under stress conditions, with increasing ATP consumption by the cell, the 6-kDa subunit accumulates; by switching on F 1 F 0 -ATP synthase it provides additional energy needed for cell homeostasis.

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“…TU has also been recognized as an efficient bioregulator imparting tolerance to crop plants against various stresses (Asthir et al 2013;Kaya et al 2015;Srivastava et al 2014). Improvement in the oxidative defense system and plant growth and development under different stress conditions due to TU application was observed in many crops like maize (Kaya et al 2013;Sahu et al 1993), wheat (Asthir et al 2013;Sahu et al 2005), pearl millet (Parihar et al 1998), and Brassica juncea (Srivastava et al 2009;Srivastava et al 2010a;Srivastava et al 2010b). TU was also reported to improve the photosystem efficiency, vegetative growth, and improved regulation of the source-sink relation in B. juncea (Pandey et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Exogenous application of plant growth regulators (PGRs) induces chilling tolerance in short-duration hybrid maize

Waqas

Khan

Akhter

et al. 2017

Environ Sci Pollut Res

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Chilling stress hampers the optimal performance of maize under field conditions precipitously by inducing oxidative stress. To confer the damaging effects of chilling stress, the present study aimed to investigate the effects of some natural and synthetic plant growth regulators, i.e., salicylic acid (SA), thiourea (TU), sorghum water extract (SWE), and moringa leaf extract (MLE) on chilling stress tolerance in autumn maize hybrid. Foliar application of growth regulators at low concentrations was carried out at six leaf (V6) and tasseling stages. An increase in crop growth rate (CGR), leaf area index (LAI), leaf area duration (LAD), plant height (PH), grain yield (GY), and total dry matter accumulation (TDM) was observed in exogenously applied plants as compared to control. In addition, improved physio-biochemical, phenological, and grain nutritional quality attributes were noticed in foliar-treated maize plots as compared to non-treated ones. SA-treated plants reduced 20% electrolyte leakage in cell membrane against control. MLE and SA were proved best in improving total phenolic, relative water (19-23%), and chlorophyll contents among other applications. A similar trend was found for photosynthetic and transpiration rates, whereas MLE and SWE were found better in improving CGR, LAI, LAD, TDM, PH, GY, grains per cob, 1000 grain weight, and biological yield among all treatments including control. TU and MLE have significantly reduced the duration in phenological events of crop at the reproductive stage. MLE, TU, and SA also improved the grain protein, oil, and starch contents as compared to control. Enhanced crop water productivity was also observed in MLE-treated plants. Economic analysis suggested that MLE and SA applications were more economical in inducing chilling stress tolerance under field conditions. Although eliciting behavior of all growth regulators improved morpho-physiological attributes against suboptimal temperature stress conditions, MLE and SA acted as leading agents which proved to be better stress alleviators by improving plant physio-biochemical attributes and maize growth.

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Thiourea modulates the expression and activity profile of mtATPase under salinity stress in seeds of Brassica juncea

Cited by 55 publications

References 41 publications

Thiourea mediates alleviation of UV-B stress-induced damage in the Indian mustard (Brassica junceaL.)

Thiourea mediates alleviation of UV-B stress-induced damage in the Indian mustard (Brassica junceaL.)

Isolation and in silico functional analysis of MtATP6, a 6-kDa subunit of mitochondrial F1F0-ATP synthase, in response to abiotic stress

Exogenous application of plant growth regulators (PGRs) induces chilling tolerance in short-duration hybrid maize

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