Third-generation oxygen amperometric biosensor based on Trametes hirsuta laccase covalently bound to graphite electrode

Gutiérrez-Sánchez, Cristina; Shleev, Sergey; Lacey, Antonio L. De; Pita, Marcos

doi:10.2478/s11696-014-0595-x

Cited by 13 publications

(9 citation statements)

References 11 publications

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“…The performance of the PGE implemented by direct linking BOx to rGO via PBSE was low (11 ± 3 µA mM −1 cm −2 , n = 3). Both PGE-rGO-MWCNT-BOx and PGE-MWCNT-BOx had higher sensitivity to oxygen when compared with other biosensors referred in the literature [27][28][29][30] . Mousty et al reported a biosensor with a sensitivity value of 470 µA mM −1 cm −2 under stirring conditions but with adding of the ABTS electron mediator 31 .…”

Section: Resultsmentioning

confidence: 78%

Nanostructured pencil graphite electrodes for application as high power biocathodes in miniaturized biofuel cells and bio-batteries

Torrinha

Jiyane

Sabela

et al. 2020

Sci Rep

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This work describes a simple method for the fabrication of an enzymatic electrode with high sensitivity to oxygen and good performance when applied as biocathode. Pencil graphite electrodes (PGE) were chosen as disposable transducers given their availability and good electrochemical response. After electrochemical characterization regarding hardness and surface pre-treatment suited modification with carbon-based nanostructures, namely with reduced graphene, MWCNT and carbon black for optimal performance was proceeded. The bioelectrode was finally assembled through immobilization of bilirubin oxidase (BOx) lashed on the modified surface of MWCNT via π–π stacking and amide bond functionalization. The high sensitivity towards dissolved oxygen of 648 ± 51 µA mM−1 cm−2, and a LOD of 1.7 µM, was achieved for the PGE with surface previously modified with reduced graphene (rGO), almost the double registered for direct anchorage on the bare PGE surface. Polarization curves resulted in an open circuit potential (OCP) of 1.68 V (vs Zn electrode) and generated a maximum current density of about 650 μA cm−2 in O2 saturated solution.

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Section: Resultsmentioning

confidence: 78%

Nanostructured pencil graphite electrodes for application as high power biocathodes in miniaturized biofuel cells and bio-batteries

Torrinha

Jiyane

Sabela

et al. 2020

Sci Rep

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“…1 mL of EDC 36 mM and 1 mL of NHS 17 mM in PB pH 6.5 were added to this solution, and the reaction was allowed to proceed for 2 h at room temperature. The membrane bioelectrode was then washed using distilled water and kept at 4 °C until use [ 38 , 39 , 40 ].…”

Section: Methodsmentioning

confidence: 99%

Flexible and Conductive Bioelectrodes Based on Chitosan-Carbon Black Membranes: Towards the Development of Wearable Bioelectrodes

et al. 2021

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Wearable sensors for non-invasive monitoring constitute a growing technology in many industrial fields, such as clinical or sport monitoring. However, one of the main challenges in wearable sensing is the development of bioelectrodes via the use of flexible and stretchable materials capable of maintaining conductive and biocompatible properties simultaneously. In this study, chitosan-carbon black (CH-CB) membranes have been synthesized using a straightforward and versatile strategy and characterized in terms of their composition and their electrical and mechanical properties. In this sense, CH-CB membranes showed good conductivity and mechanical resistance thanks to the presence of carbon black, which decreases the insulating behavior of chitosan, while flexibility and biocompatibility are maintained due to the dual composition of the membrane. Thus, flexible and biocompatible conductive bioelectrodes have been developed by the combined use of CH and CB without the use of toxic reagents, extra energy input, or long reaction times. The membranes were modified using the enzymes Glucose Oxidase and Laccase in order to develop flexible and biocompatible bioelectrodes for enzymatic glucose biofuel cells (BFCs) and glucose detection. A BFC assembled using the flexible bioelectrodes developed was able to deliver 15 µW cm−2, using just 1 mM glucose as biofuel, and up to 21.3 µW·cm−2 with higher glucose concentration. Additionally, the suitability of the CH-CB membranes to be used as a glucose sensor in a linear range from 100 to 600 µM with a limit of detection (LOD) of 76 µM has been proven. Such demonstrations for energy harvesting and sensing capabilities of the developed membrane pave the way for their use in wearable sensing and energy harvesting technologies in the clinical field due to their good mechanical, electrical, and biocompatible properties.

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“…In this work, a wireless biosensing application of DET-based oxidoreductase with conductive nanomaterials was demonstrated, and it may open new possibilities for the development of wireless biosensors. Other biosensors based on DET-capable oxidoreductases were demonstrated as well [83][84][85][86][87]. Similarly, DET-capable oxidoreductases were also used in developing third generation bioelectrodes for biofuel cells.…”

Section: Development Of Biosensors and Biofuel Cellsmentioning

confidence: 99%

Nanocatalysts Containing Direct Electron Transfer-Capable Oxidoreductases: Recent Advances and Applications

Ratautas

Dagys

2019

Catalysts

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Direct electron transfer (DET)-capable oxidoreductases are enzymes that have the ability to transfer/receive electrons directly to/from solid surfaces or nanomaterials, bypassing the need for an additional electron mediator. More than 100 enzymes are known to be capable of working in DET conditions; however, to this day, DET-capable enzymes have been mainly used in designing biofuel cells and biosensors. The rapid advance in (semi) conductive nanomaterial development provided new possibilities to create enzyme-nanoparticle catalysts utilizing properties of DET-capable enzymes and demonstrating catalytic processes never observed before. Briefly, such nanocatalysts combine several cathodic and anodic catalysis performing oxidoreductases into a single nanoparticle surface. Hereby, to the best of our knowledge, we present the first review concerning such nanocatalytic systems involving DET-capable oxidoreductases. We outlook the contemporary applications of DET-capable enzymes, present a principle of operation of nanocatalysts based on DET-capable oxidoreductases, provide a review of state-of-the-art (nano) catalytic systems that have been demonstrated using DET-capable oxidoreductases, and highlight common strategies and challenges that are usually associated with those type catalytic systems. Finally, we end this paper with the concluding discussion, where we present future perspectives and possible research directions.

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Third-generation oxygen amperometric biosensor based on Trametes hirsuta laccase covalently bound to graphite electrode

Cited by 13 publications

References 11 publications

Nanostructured pencil graphite electrodes for application as high power biocathodes in miniaturized biofuel cells and bio-batteries

Nanostructured pencil graphite electrodes for application as high power biocathodes in miniaturized biofuel cells and bio-batteries

Flexible and Conductive Bioelectrodes Based on Chitosan-Carbon Black Membranes: Towards the Development of Wearable Bioelectrodes

Nanocatalysts Containing Direct Electron Transfer-Capable Oxidoreductases: Recent Advances and Applications

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