Thirty‐Six Month Follow‐up of 25 Patients Treated With Combination Anorganic Bovine‐Derived Hydroxyapatite Matrix (ABM)/Cell‐Binding Peptide (P‐15) Bone Replacement Grafts in Human Infrabony Defects. I. Clinical Findings

Yukna, Raymond A.; Krauser, Jack T.; Callan, Donald P.; Evans, Gerald H.; Cruz, Renee; Martín, M. Gómez

doi:10.1902/jop.2002.73.1.123

Cited by 63 publications

(56 citation statements)

References 41 publications

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“…Furthermore, the 9.0 ± 7.3% of residual ABM/P-15 revealed by histomorphometry may have continued to form new bone in vivo and added to the percentage of new bone formation, thus making the difference between the mean scores of the treatments even smaller with additional time (Table 3). The successful bone formation results achieved in this study with ABM/P-15 are consistent with results obtained in other preclinical models [14][15][16], dental models [17][18][19][20][21][22][23][24][25][26], and the pilot orthopaedic model [9].…”

Section: Discussionsupporting

confidence: 89%

“…Yukna et al [26] used ABM/P-15 as a bone replacement graft in human periodontal defects and found a significant improvement at 6 months and 3 years postoperative when compared to the time of surgery. These outcomes included increased mean clinical attachment level, a decrease in probing depth, and improved mean gingival recession.…”

Section: Discussionmentioning

confidence: 99%

“…Ideally, with unlimited funding, the addition of 6 weeks and 3 month histologic evaluations would have been ideal. However, we chose 3 and 6 months as our time points for this pilot study based on previous studies reporting variable rates of fusion ranging from 3 to 12 months [9,15,18,26,29,35]. As for the high fusion rates, a control with empty PEEK rings would likely have resulted in a low fusion rate.…”

Section: Discussionmentioning

confidence: 99%

“…Both studies concluded that P-15 is an appropriate bone graft substitute to create new bone. Furthermore, ABM/P-15 has been used in humans for the regeneration of bone in periodontal defects for almost 10 years [17][18][19][20][21][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of ABM/P-15 versus autogenous bone in an ovine lumbar interbody fusion model

et al. 2010

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A prospective, randomized study was performed in an ovine model to compare the efficacy of an anorganic bovine-derived hydroxyapatite matrix combined with a synthetic 15 amino acid residue (ABM/P-15) in facilitating lumbar interbody fusion when compared with autogenous bone harvested from the iliac crest. P-15 is a biomimetic to the cell-binding site of Type-I collagen for bone-forming cells. When combined with ABM, it creates the necessary scaffold to initiate cell invasion, binding, and subsequent osteogenesis. In this study, six adult ewes underwent anterior-lateral interbody fusion at L3/L4 and L4/L5 using PEEK interbody rings filled with autogenous bone at one level and ABM/P-15 at the other level and no additional instrumentation. Clinical CT scans were obtained at 3 and 6 months; micro-CT scans and histomorphometry analyses were performed after euthanization at 6 months. Clinical CT scan analysis showed that all autograft and ABM/P-15 treated levels had radiographically fused outside of the rings at the 3-month study time point. Although the clinical CT scans of the autograft treatment group showed significantly better fusion within the PEEK rings than ABM/P-15 at 3 months, micro-CT scans, clinical CT scans, and histomorphometric analyses showed there were no statistical differences between the two treatment groups at 6 months. Thus, ABM/P-15 was as successful as autogenous bone graft in producing lumbar spinal fusion in an ovine model, and it should be further evaluated in clinical studies.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of ABM/P-15 versus autogenous bone in an ovine lumbar interbody fusion model

et al. 2010

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“…P-15 has been shown to facilitate physiological processes in a way similar to collagen, to facilitate the exchange of mechanical signals, and to promote cell differentiation [4][5][6]. Like other bone augmentation materials, P-15 associated with anorganic-derived bone matrix (ABM), has been shown to be helpful in the treatment of periodontal defects, and sinus-lifting procedures [1,[7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Early Effects of P-15 on Human Bone Marrow Stem Cells

Sollazzo¹

2010

JOMR

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Objectives: is an analogue of the cell binding domain of collagen. P-15 has been shown to facilitate physiological to process in a way similar to collagen, to serve as anchorage for cells, and to promote the binding, migration and differentiation of cells. However, how P-15 alters osteoblast activity to promote bone formation is poorly understood. To study the osteoinductive properties of peptide P-15, we analyzed the expression levels of bone related genes in human mesenchymal stem cells treated with this biomaterial. Material and methods: Using real time Reverse Transcription-Polymerase Chain Reaction the quantitative expression of specific genes, like transcriptional factors (RUNX2 and SP7), bone related genes (SPP1, COL1A1, COL3A1, BGLAP, ALPL, and FOSL1) and mesenchymal stem cells marker (ENG) were examined. Results: P-15 causes a considerable induction of osteoblast transcriptional factor like osterix (SP7) and of the bone related genes osteopontin (SPP1) and osteocalcin (BGLAP). In contrast the expression of endoglin (ENG) was markedly decreased in stem cells treated with P-15 respect to untreated cells, indicating the differentiation effect of this biomaterial on stem cells. Conclusion: The present study shows the effect of P-15 on mesenchymal stem cells in the early differentiation stages: P-15 is an inducer of osteogenesis on human stem cells as indicated by the activation of bone related markers SP7, SPP1 and BGLAP. The results may allow a better understanding of the molecular mechanism of bone regeneration and as a model for comparing other materials with similar clinical effects.

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