Rheological characterization of sludge is known to be an essential tool to optimize flow, mixing and other process parameters in wastewater treatment plants. This study deals with the characterization of thickened excess activated sludge in comparison to raw primary sludge and excess activated sludge. The effects of key parameters (total solid concentration, temperature, and pH) on the rheology and flow behaviour of thickened excess activated sludge were studied. The rheological investigations were carried out for total solid concentration range of 0.9%w/w-3.7%w/w, temperature range of 23°C-55°C, and pH range of 3.6-10.0. Different rheological model equations were fitted to the experimental data. The model equations with better fitting were used to calculate the yield stress, apparent, zerorate, infinite-rate viscosities, flow consistency index, and flow index. The decrease in concentration from 3.7%w/w to 3.1%w/w resulted in drastic reduction of yield stress from 27.6Pa to 11.0Pa, and further reduction of yield stress to 1.3Pa was observed as solid concentration was reduced to 1.3%w/w. The viscosity at higher shear rate (>600s-1) decreased from 0.05Pa.s down to 0.008Pa.s when the total solid concentration was reduced from 3.7% to 0.9%. Yield stress decreased from 20.1Pa down to 8.3Pa for Bingham plastic model when temperature was raised from 25˚C to 55˚C. Activation energy and viscosity also showed decreasing trend with increasing temperature. Yield stress of thickened excess activated sludge increased from a value of 6.0Pa to 8.3Pa when pH was increased from 3.6 to 10.0. The effect of polymer dose on the rheological behaviour of thickening of excess activated sludge was also investigated, and the optimum polymer dosage for enhanced thickener performance was determined to be 1.3kg/ton DS.