Three Different cDNAs Encoding Mouse D-Factor/LIF Receptor1

Abstract

Three cDNAs for mouse differentiation-stimulating factor (D-factor)/leukemia inhibitory factor (LIF) receptor were isolated from a cDNA library prepared from the liver of a pregnant mouse. A probe for screening was prepared by the RT-PCR method using human cDNA sequences as primers. The mouse D-factor receptor cDNA encoded 1,092 amino acids, which had a marked homology with the human counterpart and consisted of signal sequence, extracellular, transmembrane, and cytoplasmic domains. The WSXWS motif found in members of the cytokine receptor family was also present in the extracellular domain of the mouse D-factor receptor. A second form of cDNA that had a 501 bp insertion was isolated. The insertion introduced a stop codon so that the mRNA encoded the soluble receptor lacking transmembrane and intracellular domains. Because the insertion contained polyadenylation signals, two different sizes of mRNA encoding the soluble receptor were produced, depending on whether or not it utilized these signals. Transcripts utilizing these signals were 2.6-3 kb in size, and were very abundantly expressed in the liver. Transcripts that did not use these signals were longer than 5 kb and of similar size to the mRNA for the cellular receptor.

Dual control of LIF expression and LIF receptor function regulate Stat3 activation at the onset of uterine receptivity and embryo implantation

“…1C). As expected, multiple RNA transcripts, generated by alternate splicing or polyadenylation, were detected by Northern analysis on whole uteri and purified LE for both receptor components, consistent with previous reports (20,21).…”

Soluble receptors in human disease

“…1D). In this model, the soluble receptor binds ligand in the splicing IL-6R␣ [51][52][53][54][55][56] splicing, cleavage Ag gp130 [57][58][59] splicing I CNTFR [60,61] GPI Ag LIFR [62][63][64][65][66] splicing I Leptin R [67][68][69][70] splicing I IL-11R [71,72] splicing Ag, I IL-12 p40 [73][74][75][76] splicing Ag, I Stem cell factor R (c-kit) [77][78][79] combined I Interferon R [80][81] splicing Lipopolysaccharide R (CD14) [82][83][84][85] GPI I…”

Molecular cloning and functional analysis of cDNA encoding a rat leukemia inhibitory factor: towards generation of pluripotent rat embryonic stem cells

“…Leukemia inhibitory factor (LIF) is a secreted glycoprotein produced by a number of di erent cell types, including Krebs ascites (Hilton et al, 1988a,b), Ehrlich ascites (Abe et al, 1986), various ®broblasts including L929 (Tomida et al, 1984), STO (Smith and Hooper, 1983;Koopman and Cotton, 1984), bu alo rat liver cells (Smith and Hooper, 1987), antigenstimulated human alloreactive T lymphocytes (Godard et al, 1988), and mitogen-stimulated spleen cells (Abe et al, 1986). It was characterized and cloned by its ability to induce the di erentiation of the murine myeloid leukemia cell line M1 , Gearing et al, 1987.…”