Three-dimensional alteration of cervical anterior spinal artery and anterior radicular artery in rat model of chronic spinal cord compression by micro-CT

Cheng, Xing; Long, Houqing; Chen, Wenli; Xu, Jiadai; Huang, Yangliang; Fo-bao, LI

doi:10.1016/j.neulet.2015.08.050

Cited by 27 publications

(17 citation statements)

References 28 publications

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“…In our previous studies, we have reported that angiogenesis is related to the recovery of neurological function (Long et al, 2014;Cheng et al, 2015). Here, we confirm that angiogenesis serves as a beneficial factor to improve neurological function after CCSCI, which is supported by many other published studies.…”

Section: Discussionsupporting

confidence: 90%

“…In the SCI group, the polymer (1→4)-3,6-anhydro-a-l-galactopyranosyl-(1→3)-β-D-galactopyranan) sheet (1 mm × 3 mm × 1 mm) was implanted into the C6 epidural space on the dorsal part of the spinal cord. Spinal cord compression was achieved by expansion of the polymer caused by liquid absorption (Long et al, 2014;Cheng et al, 2015). This sheet can absorb liquid in the spinal canal to expand its volume sevenfold (around 2.3 mm × 4.2 mm × 2.2 mm) at the end (Kim et al, 2004;Long et al, 2013).…”

Section: Chronic Compressive Spinal Cord Injurymentioning

confidence: 99%

“…It has been reported that vascular events after SCI not only affect the progress of secondary tissue damage but also establish a new environment that may promote neural plasticity in the chronically injured spinal cord (Mautes et al, 2000;Wang et al, 2018). We have previously revealed that the extent of angiogenesis is related to neurological function recovery following chronic compressive SCI (Long et al, 2014;Cheng et al, 2015). Zoe et al, reported that loss of proliferating NG2 + pericytes prevents intra-lesion angiogenesis and influences scar-forming processes, impairing endogenous repair after SCI in mice (Hesp et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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LncRNA Xist Contributes to Endogenous Neurological Repair After Chronic Compressive Spinal Cord Injury by Promoting Angiogenesis Through the miR-32-5p/Notch-1 Axis

Cheng

et al. 2020

Front. Cell Dev. Biol.

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Endogenous repair after chronic compressive spinal cord injury (CCSCI) is of great clinical interest. Ischemia-hypoxia-induced angiogenesis has been proposed to play an important role during this repair process. Emerging evidence indicates that long non-coding RNAs (lncRNAs) are involved in the pathophysiological processes of various diseases. Here, we identified a lncRNA (Xist; X-inactive specific transcript) with upregulated expression in cervical spine lesions during endogenous neurological repair in CCSCI rats. Therapeutically, the introduction of Xist to rats increased neurological function in vivo as assayed using the Basso, Beattie, and Bresnahan (BBB) score and inclined plane test (IPT). We found that the introduction of Xist enhanced endogenous neurological repair by promoting angiogenesis and microvessel density after CCSCI, while depletion of Xist inhibited angiogenesis and cell sprouting and migration. Mechanistically, Xist promoted angiogenesis by sponging miR-32-5p and modulating Notch-1 expression both in vitro and in vivo. These findings suggest a role of the Xist/miR-32-5p/Notch-1 axis in endogenous repair and provide a potential molecular target for the treatment of ischemia-related central nervous system (CNS) diseases.

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Section: Discussionsupporting

confidence: 90%

Section: Chronic Compressive Spinal Cord Injurymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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LncRNA Xist Contributes to Endogenous Neurological Repair After Chronic Compressive Spinal Cord Injury by Promoting Angiogenesis Through the miR-32-5p/Notch-1 Axis

Cheng

et al. 2020

Front. Cell Dev. Biol.

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“…Our previous study using a rat model indicated that the most serious neurological damage occurred at the 28th day after chronic compression (Cheng et al, 2015 ) and was accompanied by reduction of micro vessels in the dorsal gray matter of cervical cord (Long et al, 2014 ; Cheng et al, 2015 ). Simultaneously, high permeability along with flattening and widening of the compressive zone/segment was found (Long et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural Features of Neurovascular Units in a Rat Model of Chronic Compressive Spinal Cord Injury

Long

Chen

et al. 2018

Front. Neuroanat.

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Chronic spinal cord compression is the most common cause of spinal cord impairment worldwide. Objective of this study is to assess the ultrastructural features of the neurovascular unit (NVU) in a rat model of chronic compressive spinal cord injury, 24 SD rats were divided into two groups: the control group (n = 12), and the compression group (n = 12). A C6 semi-laminectomy was performed in the control group, whereas a water-absorbent polyurethane polymer was implanted into the C6 epidural space in the compression group. The Basso Beattie Bresnahan (BBB) scores and the somatosensory evoked potentials (SEP) were used to evaluate neurological functions. Transmission Electron Microscopy (TEM) was performed to investigate the change of NVU at the 28th day after modeling. Compared with the control group, the compression group shows a significant reduction (P < 0.05) of BBB score and a significant severity (P < 0.05) of abnormal SEP. TEM results of the compression group showed a striking increase in endothelial caveolae and vacuoles; a number of small spaces in tight junctions; a significant increase in pericyte processing area and vessel coverage; an expansion of the basement membrane region; swollen astrocyte endfeet and mitochondria; and the degeneration of neurons and axons. Our study revealed that damage to NVU components occurred followed by chronic compressive spinal cord injury. Several compensatory changes characterized by thicker endothelium, expansive BM, increased pericyte processing area and vessel coverage were also observed.

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“…Cheng et al [11] and Long et al [12] reported the use of the lead oxide combined with micro-CT scanning for cervical vascular changes in a rat chronic compression model, but the microvascular perfusion was relatively poor. The present study further showed that the microvascular perfusion of cervical spinal cord was not ideal with the lead oxide at low-resolution or high-resolution micro-CT scanning.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Three-Dimensional Micro-CT Angiography of Cervical Spinal Cord between Two Contrast Agents

Liu

Zhou

et al. 2019

Contrast Media & Molecular Imaging

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Purpose. Barium sulfate and lead oxide are commonly used for angiographic studies, but there is no report on the comparison of two contrast agents in angiography of cervical spinal cord. This study was aimed to compare the microvascular architecture of cervical spinal cord in rats after angiography with the barium sulfate agent to the lead oxide agent. Methods. Twelve adult Sprague-Dawley rats were randomly divided into the barium sulfate group (n=6) and the lead oxide group (n=6). Each rat was perfused under the same protocol using either two contrast agents. The angiography was evaluated with the vascular number at different ranks. The cervical spinal cord samples were scanned using micro-CT with low resolution and high resolution. The microvascular parameters, including ratio of vascular volume to tissue volume (VV/TV), vascular number (V.N), diameter (V.Dm), separation (V.Sp), connectivity density (Conn.D), structure model index (SMI), percentage, and volume of vessels at different diameters were measured. Results. The perfusion was better in the barium sulfate group, with more blood vessel trees of rank II and III visible compared to the lead oxide group. Low-resolution micro-CT analysis showed no difference in microvascular parameters except SMI between the two groups. High-resolution micro-CT analysis results showed that V.N and Conn.D of barium sulfate group were 60% and 290% more than those of the lead oxide group; however, V.Sp was 41% less than the lead oxide group. The percentage of vessels with diameter of 10 μm and 20 μm, and the volume of vessels with diameter of less than 100 μm was higher in the barium sulfate group than in the lead oxide group. The SMI index in the barium sulfate group was higher than that in the lead oxide group at both low resolution and high resolution. Conclusions. Compared with lead oxide, barium sulfate is more suitable for perfusion of cervical spinal cord microvessels, and cheap and nontoxic with high resolution.

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Three-dimensional alteration of cervical anterior spinal artery and anterior radicular artery in rat model of chronic spinal cord compression by micro-CT

Cited by 27 publications

References 28 publications

LncRNA Xist Contributes to Endogenous Neurological Repair After Chronic Compressive Spinal Cord Injury by Promoting Angiogenesis Through the miR-32-5p/Notch-1 Axis

LncRNA Xist Contributes to Endogenous Neurological Repair After Chronic Compressive Spinal Cord Injury by Promoting Angiogenesis Through the miR-32-5p/Notch-1 Axis

Ultrastructural Features of Neurovascular Units in a Rat Model of Chronic Compressive Spinal Cord Injury

Comparison of Three-Dimensional Micro-CT Angiography of Cervical Spinal Cord between Two Contrast Agents

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