Three-dimensional analysis of combination effect of ellagitannins and acyclovir on herpes simplex virus types 1 and 2

Vilhelmova, Neli; Jacquet, Rémi; Quideau, Stéphane; Stoyanova, Adelina; Galabov, Angel S.

doi:10.1016/j.antiviral.2010.11.014

Cited by 27 publications

(23 citation statements)

References 34 publications

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“…The degree of interaction is expressed for data represented as percentages in which 0–25, 25–50, 50–100 and >100 μM/ml 2 % calculated values in either a positive (synergy) or negative (antagonism) direction are defined as insignificant, minor, moderate and strong interaction respectively (19–21). …”

Section: Methodsmentioning

confidence: 99%

NVP-BEZ235, a dual PI3K/mTOR inhibitor synergistically potentiates the antitumor effects of cisplatin in bladder cancer cells

Moon

Lee

et al. 2014

International Journal of Oncology

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The PI3K/Akt/mTOR pathway is a prototypic survival pathway and constitutively activated in many malignant conditions. Moreover, activation of the PI3K/Akt/mTOR pathway confers resistance to various cancer therapies and is often associated with a poor prognosis. In this study, we explored the antitumor effect of NVP-BEZ235, a dual PI3K/mTOR inhibitor in cisplatin-resistant human bladder cancer cells and its synergistic interaction with cisplatin. A human bladder cancer cell line with cisplatin resistance was exposed to escalating doses of NVP-BEZ235 alone or in combination with cisplatin and antitumor effects was determined by the CCK-8 assay. Based on a dose-response study, synergistic interaction between NVP-BEZ235 and cisplatin was evaluated by combination index (CI), three-dimensional model and clonogenic assay. The combination of NVP-BEZ235 and cisplatin caused significant synergistic antitumor effect in cisplatin-resistant bladder cancer cells over a wide dose range and reduced the IC50 of NVP-BEZ235 and cisplatin by 5.6- and 3.6-fold, respectively. Three-dimensional synergy analysis resulted in a synergy volume of 388.25 μM/ml2% indicating a strong synergistic effect of combination therapy. The combination therapy caused cell cycle arrest and caspase-dependent apoptosis. Although NVP-BEZ235 suppressed PI3K/mTOR signaling without any paradoxical induction of Akt activity, it caused MEK/ERK pathway activation. The present study demonstrated that the PI3K/mTOR dual inhibitor NVP-BEZ235 can synergistically potentiate the antitumor effects of cisplatin in cisplatin-resistant bladder cancer cells though the suppression of cell cycle progression and the survival pathway as well as induction of caspase-dependent apoptosis.

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Section: Methodsmentioning

confidence: 99%

NVP-BEZ235, a dual PI3K/mTOR inhibitor synergistically potentiates the antitumor effects of cisplatin in bladder cancer cells

Moon

Lee

et al. 2014

International Journal of Oncology

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“…In previous studies, we have shown that castalagin possesses selective activity against the replication of ACVsusceptible strains of HSV-1and HSV-2, and its activity is even higher than that of ACV on the HSV-1 strain [13]. The combination of castalagin with ACV manifested a pronounced synergicity.…”

Section: Discussionmentioning

confidence: 99%

“…In previous investigations, we have shown that three C-glucosidic ellagitannins, namely castalagin, vescalagin, and grandinin, demonstrate remarkable activity against human HSV-1 and HSV-2 that are sensitive or resistant to acyclovir strains [13,21]. In addition, when combined with acyclovir, they manifested a marked synergism [13].…”

Section: Introductionmentioning

confidence: 99%

“…Castalagin demonstrated the highest anti-HSV-1 activity among the tested ellagitannins, attaining effi cacy equal to acyclovir [13,21]. Thus, we used castalagin to study the mode of anti-herpesvirus action of these ellagitannin compounds.…”

Section: Introductionmentioning

confidence: 99%

“…Hydrolyzable tannins, such as ellagitannins and gallotannins, can be hydrolyzed into glucose, gallic and ellagic acids [10][11][12][13]. Ellagitannins are potential inhibitors of various enveloped viruses due to their ability to bind with different proteins, thus altering the proteins structure and inactivating them [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

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Castalagin: Some aspects of the mode of Anti-Herpes Virus Activity

As¹

2018

Ann Antivir Antiretrovir

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Castalagin, a nonahydroxytriphenoyl-bearing C-glucosidic ellagitannin, manifested a marked virucidal effect on HSV-1. This effect was markedly temperature and time dependent, being clearly higher at 37 °C than at room temperature: ∆log of 3.13 with 10 μM concentration of castalagin (MNC). Castalagin also suppressed HSV-1 particle attachment to MDBK cells. A signifi cant effect (∆log = 1.7) was found after 30 min of substance exposure and was increased at 60 min (∆log = 3.2). Castalagin effect on the production of virions during the HSV-1 replication cycle was studied using a time-of-addition experimental scheme at the one-step viral replication cycle design. The highest sensitivity to castalagin was recorded 0-3 h post virus inoculation. A substantially weaker effect was found at the 6-12 h time intervals. At 18-24 h, HSV-1 replication was unsusceptible to castalagin. Evidently, castalagin target is located in the earlier stages of the HSV-1 replication cycle.

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Palladium‐Catalysed Cross‐Coupling of Vinyldisiloxanes with Benzylic and Allylic Halides and Sulfonates

Frye

Connor

Twigg

et al. 2012

Chemistry A European J

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The Hiyama cross-coupling reaction is a powerful method for carbon-carbon bond formation. To date, the substrate scope of this reaction has predominantly been limited to sp(2)-sp(2) coupling reactions. Herein, the palladium-catalysed Hiyama type cross-coupling of vinyldisiloxanes with benzylic and allylic bromides, chlorides, tosylates and mesylates is reported. A wide variety of functional groups were tolerated, and the synthetic utility of the methodology was exemplified through the efficient total synthesis of the cytotoxic natural product bussealin A. In addition, the antiproliferative ability of bussealin A was evaluated in two cancer-cell lines.

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Three-dimensional analysis of combination effect of ellagitannins and acyclovir on herpes simplex virus types 1 and 2

Cited by 27 publications

References 34 publications

NVP-BEZ235, a dual PI3K/mTOR inhibitor synergistically potentiates the antitumor effects of cisplatin in bladder cancer cells

NVP-BEZ235, a dual PI3K/mTOR inhibitor synergistically potentiates the antitumor effects of cisplatin in bladder cancer cells

Castalagin: Some aspects of the mode of Anti-Herpes Virus Activity

Palladium‐Catalysed Cross‐Coupling of Vinyldisiloxanes with Benzylic and Allylic Halides and Sulfonates

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