Three-dimensional assessment of proximal contact enamel using optical coherence tomography

Zin, Thwe; Shimada, Yasushi; Abdou, Ahmed; Sadr, Alireza; Yoshiyama, Masahiro; Sumi, Yasunori; Tagami, Junji

doi:10.1016/j.dental.2019.01.008

Cited by 11 publications

(8 citation statements)

References 38 publications

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“…Swept-source OCT uses laser source that emits different wavelengths sequentially in ultrahigh speed at a kilohertz rate to acquire real-time imaging with enhanced image resolution and 3D image construction. Optical coherence tomography has been used in the detection of carious lesion, cracks, and dental restoration defects [14][15][16][17][18][19]. Previous study validated early caries detection under OCT with histological and confocal laser scanning microscope (CLSM) [19].…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of Occlusal Caries with Dynamic Slicing of 3D Optical Coherence Tomography Images

Luong

Shimada

Araki

et al. 2020

Sensors

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Detecting the extent of occlusal caries is a clinically important but challenging task required for treatment decision making. The aim of this study was to assess the diagnostic power of 3D swept-source optical coherence tomography (OCT) for evaluation of occlusal caries in comparison with X-ray radiography. Extracted human molars not exhibiting American Dental Association (ADA) criteria advanced caries were mounted in a silicone block and digital dental radiographs were captured from the buccal side. Subsequently, occlusal surfaces were scanned with a prototype Yoshida Dental OCT. Thirteen examiners evaluated the presence and extent of caries on radiographs and dynamically sliced 3D OCT video images, using a 4 level scale—0: intact; 1: enamel demineralization without cavitation; 2: enamel caries with cavitation; 3: dentin caries with or without cavitation. Sensitivity, specificity and area under operating characteristic curves (Az) were statistically analyzed (α = 0.05). Reliability analysis showed an excellent agreement among the 13 examiners for both methods. The OCT presented a significantly higher sensitivity and Az value for the detection of caries compared to radiographs (p < 0.05). Radiography showed especially low sensitivity for dentin caries (0–2 versus 3). Dynamic slicing of 3D OCT volumes is a powerful adjunct tool to visual inspection to diagnose the dentin occlusal caries in vitro.

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Section: Introductionmentioning

confidence: 99%

Diagnosis of Occlusal Caries with Dynamic Slicing of 3D Optical Coherence Tomography Images

Luong

Shimada

Araki

et al. 2020

Sensors

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“…The micromorphologies of the mineralized tissues in enamel are frequently measured by electron microscopy or computed tomography-based technologies which, however, suffer from ionizing radiation hazards, expensive devices as well as sophisticated sample preparation [9]. Noninvasive spectroscopic techniques such as optical coherence tomography (OCT) and Raman spectroscopy have recently been developed as powerful analytical tools for the mineralized microstructures of tooth organs [10][11][12][13][14][15][16]. OCT is a nondestructive, cross-sectional imaging tool that is capable of supplying depth-resolved structural information of dental enamel with micrometer resolution [10].…”

Section: Introductionmentioning

confidence: 99%

“…Noninvasive spectroscopic techniques such as optical coherence tomography (OCT) and Raman spectroscopy have recently been developed as powerful analytical tools for the mineralized microstructures of tooth organs [10][11][12][13][14][15][16]. OCT is a nondestructive, cross-sectional imaging tool that is capable of supplying depth-resolved structural information of dental enamel with micrometer resolution [10]. It has been reported that the OCT signals are closely correlated with the demineralization/remineralization cycles, which facilitates the quantitative analysis of the biomineralization process in enamel under different pathophysiological conditions [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Quantitative label‐free optical technique to analyze the ultrastructure changes and spatiotemporal relationship of enamel induced by Msx2 deletion

Líu

Guo

et al. 2021

Journal of Biophotonics

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New advances in the molecular mechanism of enamel mineralization reveal the practical significance of regenerative medicine in clinical transformation. Muscle segment homeobox 2 (MSX2), a transcription factor, is recently reported to be closely associated with the amelogenesis imperfecta (AI). To elucidate the biomineralization framework of AI enamel, herein, Msx2 gene mutant mice are investigated by dual-mode noninvasive spectroscopic analytical techniques for the first time. Optical coherence tomography (OCT) records the depth-resolved structural information of mice teeth, where a dramatic decrease in enamel thickness and quality occurred in Msx2 deficient (Msx2 À/À ) enamel. And it has the advantages of fast, noninvasive and low cost. Raman spectroscopy, a powerful molecular fingerprint tool, further witnesses an imbalance of inorganic and organic contents in Msx2 À/À enamel. In addition, abnormal expression of MSX2 also influences the spatial distribution of phosphate in enamel according to the Raman spectral imaging. Therefore, OCT integrated with Raman spectroscopy provides the quantitative label-free optical parameters of both the physical structure and chemical component in mice enamel, which strengthens the understanding of the biomineralization process underlying the Msx2-related amelogenesis imperfect.

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“…Indeed, continuously decreasing cross-sectional hardness values of demineralized and infiltrated enamel have previously been shown with increasing lesion depths [23], and this would render non-infiltrated enamel lesion areas vulnerable. Moreover, it has recently been shown that the level of demineralization correlates with the presence of superficial microcracks within the vicinities of proximal contact areas [28]; hence, it does not seem surprising that the prevalence of marginal ridge fractures is associated with the presence of proximal carious lesions [29]. To overcome this fragility, a more comprehensive attempt to adhesively reinforce the enamel lesion would seem favorable [30,31], and, therefore, a combined external/internal infiltration concept increasing the amount of infiltrated lesion volume has recently been introduced [32].…”

Section: Introductionmentioning

confidence: 99%

Ex vivo investigation on internal tunnel approach/internal resin infiltration and external nanosilver-modified resin infiltration of proximal caries exceeding into dentin

Kielbassa¹,

Leimer²,

Hartmann³

et al. 2020

PLoS ONE

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This ex vivo proof-of-concept study aimed to investigate the effect of nanosilver particles (AgNP) added to a conventional infiltrant resin (Icon) on external penetration into natural proximal enamel caries exceeding into dentin after internal tunnel preparation and internal infiltration. Carious lesions (ICDAS codes 2/3) of extracted human (pre-)molars revealing proximal caries radiographically exceeding into dentin (E2/D1 lesions) were preselected. Then, 48 of those specimens showing demineralized areas transcending the enamel-dentin border as assessed by means of near-infrared light transillumination (DIAGNOcam) were deproteinized (NaOCl, 5%). Using an internal tunnel approach, occlusal cavities central to the marginal ridge were prepared. Excavation of carious dentin, total etch procedure (H 3 PO 4 , 40%), and internal resin infiltration (FITC-labeled) followed, along with final restorations (flowable composite resin). Outer lesion surfaces were etched (HCl, 15%) prior to external infiltration (RITC-labeled). Group 1 (control; n = 24) used non-modified infiltrant, while an infiltrant/ AgNP mixture (20 nm; 5.5 wt%) was used with experimental Group 2 (n = 24). Non-infiltrated pores of cut lesions were stained (Berberine), and specimens were analyzed using confocal laser scanning microscopy. Compared to the non-filled infiltrant, incorporation of AgNP had no effect on the resin's external penetration. Between the groups, no significant differences regarding internal or external infiltration could be detected, and non-infiltrated lesion areas did not differ significantly (p>0.109; t-test). The internal tunnel preparation in combination with both an internal resin infiltration and an additional external infiltration approach using a nanosilver-modified infiltrant resin leads to increased infiltrated lesion areas, thus occluding and adhesively stabilizing the porous volume of the demineralized enamel. While exerting antimicrobial effects by the nanosilver particles, this approach should have the potential as a viable treatment alternative for proximal lesions extending into dentin, thus avoiding the sacrifice of sound enamel, postponing the frequently inevitable restoration/re-restoration cycle of conventional proximal caries treatment, and improving dental health.

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Three-dimensional assessment of proximal contact enamel using optical coherence tomography

Cited by 11 publications

References 38 publications

Diagnosis of Occlusal Caries with Dynamic Slicing of 3D Optical Coherence Tomography Images

Diagnosis of Occlusal Caries with Dynamic Slicing of 3D Optical Coherence Tomography Images

Quantitative label‐free optical technique to analyze the ultrastructure changes and spatiotemporal relationship of enamel induced by Msx2 deletion

Ex vivo investigation on internal tunnel approach/internal resin infiltration and external nanosilver-modified resin infiltration of proximal caries exceeding into dentin

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