Three-Dimensional Bio-Printed Autologous Omentum Patch Ameliorates Unilateral Ureteral Obstruction-Induced Renal Fibrosis

Jo, Hyunwoo; Choi, Bo Young; Jang, Giup; Lee, Jung Pyo; Cho, Ara; Kim, Boyun; Park, Jeong Hwan; Lee, Jeonghwan; Kim, Young Hoon; Ryu, Jina

doi:10.1089/ten.tec.2022.0165

Cited by 6 publications

(15 citation statements)

References 52 publications

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“…Autologous omental tissue was collected from the animals and processed as bio‐ink. During this process, omentum and fibrin glue were mixed at an optimized ratio (60% of omentum) to fabricate the patch based on a previous optimization study 12 . A 30 × 40 × 0.8‐mm (width × length × thickness) patch was produced using 3D bioprinting technology.…”

Section: Resultsmentioning

confidence: 99%

“…In previous study, we confirmed that the omentum patch had antifibrosis and antitubular injury effects on kidneys of chronic kidney disease rodent models. 12 In order to apply the patch to human clinical trials, we evaluated the safety of patch transplantation and found there were no immune responses or surgical complications after patch transplantation. Omentum was made in the form of a patch using 3D bioprinter after the micronization process.…”

Section: Discussionmentioning

confidence: 99%

“…Because adipocytes and milky spots are randomly distributed in native omentum, the active ingredients can be applied unevenly when applied as a patch. In the previous study, the native omentum was micronized to form a uniform patch, and the patch was transplanted in the kidneys to provide evenly active biomaterials 12 . In addition, the patch was made by mixing with fibrin glue that is degradable in vivo 22 .…”

Section: Discussionmentioning

confidence: 99%

“… 11 In the previous rodent‐model study, the patch made of micronized omentum reduced tubular injury by reducing the gene expression of the renal injury marker, such as Havcr1, Lcn2, and Ccl2. 12 In addition, the patch showed an effect on reducing renal interstitial fibrosis. After ureteral obstruction, fibrosis‐related genes were observed to be downregulated by the omentum patch, and lower fibrosis was observed in the omentum patch implant group compared to the fibrin patch group.…”

Section: Introductionmentioning

confidence: 98%

“…For these reasons micronized fat is used in dermatology, plastic surgery, and orthopedics as a source of regeneration 11 . In the previous rodent‐model study, the patch made of micronized omentum reduced tubular injury by reducing the gene expression of the renal injury marker, such as Havcr1, Lcn2, and Ccl2 12 . In addition, the patch showed an effect on reducing renal interstitial fibrosis.…”

Section: Introductionmentioning

confidence: 99%

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Safety assessment of a three‐dimensional‐printed autologous omentum patch: An application on the kidneys as a new treatment approach

Kim²,

Kim³

et al. 2023

Clinical Translational Sci

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Recently, the field of regenerative medicine has made great strides in the development of new treatments for various organ dysfunctions. One of the most promising new approaches is the use of three‐dimensional (3D) printing and autologous tissues. In this study, we evaluated the safety of a 3D‐printed autologous omentum patch to kidneys using large animals. A total of seven micropigs underwent transplantation of the 3D‐printed autologous omentum patch. Twelve weeks after transplantation, the safety was evaluated by measuring body weight, blood, and the renal resistive index. In addition, biopsy samples were histologically analyzed. The results showed no surgical complications, renal functional hematological changes, or inflammatory responses. Therefore, this study provides important insights into direct therapy to kidneys with a 3D‐printed patch made of autologous tissue. Furthermore, it has the potential for the development of new therapies for various organ dysfunction.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Safety assessment of a three‐dimensional‐printed autologous omentum patch: An application on the kidneys as a new treatment approach

Kim²,

Kim³

et al. 2023

Clinical Translational Sci

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Advances and results in omental patch repair of gastrointestinal perforations: A narrative review

Velasco-Velasco,

Llerena-Velastegui

2024

Surgery in Practice and Science

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The Use of Autologous Omentum Transposition as a Therapeutic Intervention to Reduce the Complication of Ischemia/Reperfusion Injuries in a Rat Model

Shamshirgaran,

Mohammadi,

Zahmatkesh

et al. 2024

Can J Kidney Health Dis

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Background: Ischemia/reperfusion injury (IRI) causes cellular dysfunction and death in organs like the kidney, heart, and brain. It involves energy depletion during ischemia and oxidative stress, inflammation, and apoptosis during reperfusion. Kidney IRI often leads to acute kidney injury (AKI) in various clinical scenarios. The omentum, an adipose tissue with healing properties, has been used to treat injuries in different organs. Objective: This study aimed to assess the omentum’s healing effects on reducing IRI’s adverse effects after renal ischemia in Wistar rats. Method: A total number of 36 male Wistar rats were used in a study on IRI-induced AKI. Rats were divided into 6 groups of normal kidneys wrapped with omentum “Sham-1” and “Sham-2,” ischemic kidney wrapped with omentum as “OMT-1” and “OMT-2,” and ischemic kidney without omentum as “Control-1” and “Control-2.” Ischemia was induced by clamping the left renal artery for 45 minutes. The omentum was transposed onto the injured kidney in “OMT” group. After sacrifice at weeks 4 and 8, kidney histology and blood samples were analyzed for kidney function markers. Results: On the first day after surgery, there was an immediate increase in creatinine and blood urea nitrogen (BUN) levels, which then decreased by day 28. Both OMT groups showed significantly lower levels of creatinine and BUN compared to Control groups on day 1, but after 28 days differences were not statistically significant. Histological analysis using H&E and Masson’s trichrome staining revealed significantly higher levels of inflammatory cell infiltration and hyperemia in the OMT groups. However, fibrosis and glomerular shrinkage were higher in the Control groups. Conclusion: Using an omental flap significantly prevented fibrosis within the renal parenchyma, slow down the AKI progression, and potentially serving as a promising therapeutic strategy for kidney dysfunction.

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Three-Dimensional Bio-Printed Autologous Omentum Patch Ameliorates Unilateral Ureteral Obstruction-Induced Renal Fibrosis

Cited by 6 publications

References 52 publications

Safety assessment of a three‐dimensional‐printed autologous omentum patch: An application on the kidneys as a new treatment approach

Safety assessment of a three‐dimensional‐printed autologous omentum patch: An application on the kidneys as a new treatment approach

Advances and results in omental patch repair of gastrointestinal perforations: A narrative review

The Use of Autologous Omentum Transposition as a Therapeutic Intervention to Reduce the Complication of Ischemia/Reperfusion Injuries in a Rat Model

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