Three-Dimensional Bioprinting of Perfusable Hierarchical Microchannels with Alginate and Silk Fibroin Double Cross-linked Network

Li, Huan; Li, Ningning; Zhang, He; Zhang, Yifan; Suo, Hairui; Xu, Mingzhen

doi:10.1089/3dp.2019.0115

Cited by 30 publications

(21 citation statements)

References 30 publications

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“…Among the various seaweed-derived polysaccharides, alginic acid (and particularly its salt form, sodium alginate) [ 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 ], carrageenan [ 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 ], and agarose [ 75 , 76 , 77 , 78 , 79 ] have been widely used as polymeric matrices (either solely or in combination with other polysaccharides or proteins) for the development of hydrogel-based bioinks for 3D bioprinting, as outlined in Table 1 .…”

Section: Polysaccharide-based Hydrogel Bioinksmentioning

confidence: 99%

Section: Polysaccharide-based Hydrogel Bioinksmentioning

confidence: 99%

“…The combination of alginate with nanofibrillar bio-based materials such as protein-based nanofibers has also been used with the same purpose, with a special focus on silk fibroin (SF), a natural biomaterial extracted from Bombyx mori silkworms, known for its excellent biological properties and good mechanical performance [ 61 , 66 ]. As an illustration, Li et al [ 61 ] explored the combination of SF fibers with alginate and Pluronic F127 (used as a sacrificial polymer) to produce a vascularized liver mimetic tissue. The addition of 5% ( w / v ) of SF to the bioink formulation of alginate (5% ( w / v )) and Pluronic F127 (13% ( w / v )) not only promoted a 99.5% cell viability of liver cancer cells (C3A) 14 days after printing, but the printed scaffolds of alginate and SF also showed improved mechanical properties, with compressive modulus increasing from 11.5 ± 1.6 kPa (for alginate) to 16.0 ± 2.5 kPa (for alginate/SF) [ 61 ].…”

Section: Polysaccharide-based Hydrogel Bioinksmentioning

confidence: 99%

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A Guide to Polysaccharide-Based Hydrogel Bioinks for 3D Bioprinting Applications

Teixeira

Lameirinhas

Carvalho

et al. 2022

IJMS

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Three-dimensional (3D) bioprinting is an innovative technology in the biomedical field, allowing the fabrication of living constructs through an approach of layer-by-layer deposition of cell-laden inks, the so-called bioinks. An ideal bioink should possess proper mechanical, rheological, chemical, and biological characteristics to ensure high cell viability and the production of tissue constructs with dimensional stability and shape fidelity. Among the several types of bioinks, hydrogels are extremely appealing as they have many similarities with the extracellular matrix, providing a highly hydrated environment for cell proliferation and tunability in terms of mechanical and rheological properties. Hydrogels derived from natural polymers, and polysaccharides, in particular, are an excellent platform to mimic the extracellular matrix, given their low cytotoxicity, high hydrophilicity, and diversity of structures. In fact, polysaccharide-based hydrogels are trendy materials for 3D bioprinting since they are abundant and combine adequate physicochemical and biomimetic features for the development of novel bioinks. Thus, this review portrays the most relevant advances in polysaccharide-based hydrogel bioinks for 3D bioprinting, focusing on the last five years, with emphasis on their properties, advantages, and limitations, considering polysaccharide families classified according to their source, namely from seaweed, higher plants, microbial, and animal (particularly crustaceans) origin.

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Section: Polysaccharide-based Hydrogel Bioinksmentioning

confidence: 99%

Section: Polysaccharide-based Hydrogel Bioinksmentioning

confidence: 99%

Section: Polysaccharide-based Hydrogel Bioinksmentioning

confidence: 99%

See 1 more Smart Citation

A Guide to Polysaccharide-Based Hydrogel Bioinks for 3D Bioprinting Applications

Teixeira

Lameirinhas

Carvalho

et al. 2022

IJMS

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show abstract

“…The anisotropic features in silk nanofiber scaffolds with hierarchical ECM-like morphology effectively promoted cell migration and the healing of full-thickness skin wounds ( Figure 7A ) ( Lu et al, 2018 ). Perfusable alginate/silk fibroin (Alg/SF) scaffolds with complex microfluidic channels were fabricated using the coaxial extrusion-based bioprinting method ( Li et al, 2020a ). These interconnected microchannels play an essential role in elevating cell viability and facilitating the vascularization process ( Li et al, 2020a ).…”

Section: Applicationsmentioning

confidence: 99%

“…Perfusable alginate/silk fibroin (Alg/SF) scaffolds with complex microfluidic channels were fabricated using the coaxial extrusion-based bioprinting method ( Li et al, 2020a ). These interconnected microchannels play an essential role in elevating cell viability and facilitating the vascularization process ( Li et al, 2020a ). Similarly, silk fibroin–chitosan (SF-CS) scaffolds were designed and fabricated with predefined microfluidic channels and porous structures using the ice-template-induced (ITI) method.…”

Section: Applicationsmentioning

confidence: 99%

Engineering Natural and Recombinant Silks for Sustainable Biodevices

et al. 2022

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Silk fibroin (SF) is a structural protein derived from natural silkworm silks. Materials fabricated based on SF usually inherit extraordinary physical and biological properties, including high mechanical strength, toughness, optical transparency, tailorable biodegradability, and biocompatibility. Therefore, SF has attracted interest in the development of sustainable biodevices, especially for emergent bio-electronic technologies. To expand the function of current silk devices, the SF characteristic sequence has been used to synthesize recombinant silk proteins that benefit from SF and other functional peptides, such as stimuli-responsive elastin peptides. In addition to genetic engineering methods, innovated chemistry modification approaches and improved material processing techniques have also been developed for fabricating advanced silk materials with tailored chemical features and nanostructures. Herein, this review summarizes various methods to synthesize functional silk-based materials from different perspectives. This review also highlights the recent advances in the applications of natural and recombinant silks in tissue regeneration, soft robotics, and biosensors, using B. mori SF and silk-elastin-like proteins (SELPs) as examples.

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Cross‐Linking Strategies for Silk‐Protein‐Based Inks for 3D Printing

Dong,

Liu,

Wang

et al. 2023

ChemistrySelect

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Due to excellent biocompatibility, sufficient raw material, robust mechanical properties and easy cross‐linking, Silk Fibroin (SF) is a promising protein for 3D printing inks and an ideal candidate for 3D scaffolds in fields like regenerative medicine, bioelectronics and bio‐optics. In order to meet the requirements of print accuracy, mechanical properties and form retention capabilities, the first step is to prepare SF 3D printing inks using physical, chemical or other strategies of cross‐linking. The basic chemical groups and physical structure of SF determines its ability to form 3D networks under different conditions using various cross‐linking strategies. In the preparation of SF‐based 3D printing inks, physical, chemical or other strategies of cross‐linking improve the qualities of printing, but each strategy has its advantages and disadvantages. This paper discusses different crosslinking strategies for SF to support the development of exciting potential for SF‐based 3D printing inks to meet more needs in the future.

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Three-Dimensional Bioprinting of Perfusable Hierarchical Microchannels with Alginate and Silk Fibroin Double Cross-linked Network

Cited by 30 publications

References 30 publications

A Guide to Polysaccharide-Based Hydrogel Bioinks for 3D Bioprinting Applications

A Guide to Polysaccharide-Based Hydrogel Bioinks for 3D Bioprinting Applications

Engineering Natural and Recombinant Silks for Sustainable Biodevices

Cross‐Linking Strategies for Silk‐Protein‐Based Inks for 3D Printing

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