Three‐dimensional cartilage tissue engineering using adult stem cells from osteoarthritis patients

Kafienah, Wáel; Mistry, Sanjay; Dickinson, Sally C.; Sims, Trevor J.; Learmonth, Ian D.; Hollander, Anthony P.

doi:10.1002/art.22285

Cited by 155 publications

(153 citation statements)

References 48 publications

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“…Furthermore, it is known that TGF stimulation of MSCs promotes hypertrophy and the increased expression of collagen type I and X. However, Kafienah et al 37 have shown that including parathyroid hormone-related protein downregulates collagen type I and X in cartilage tissue engineered from MSCs. It should be also noted that some transcription factors such as Sox-9 (which is known to be essential for the full expression of chondrocyte phenotype) and Wnt are not chondrogenic itself, but can make cells more responsive to growth factors and other chondrogenic stimuli.…”

Section: Discussionmentioning

confidence: 99%

Articular cartilage repair by genetically modified bone marrow aspirate in sheep

et al. 2010

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Section: Discussionmentioning

confidence: 99%

Articular cartilage repair by genetically modified bone marrow aspirate in sheep

et al. 2010

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“…We have previously shown that these methods are unable to accurately quantify collagen and proteoglycan deposition and matrix formation. 45 Kafienah et al 46 have demonstrated that MSCs isolated from a range of donors with OA (42-90 years old) are capable of undergoing chondrogenesis and produced 3-dimensional tissue engineered cartilage with an extracellular matrix composed of proteoglycan and type II collagen. It is apparent that MSCs isolated from individuals with OA possess some chondrogenic potential.…”

Section: The Effect Of Osteoarthritis On Chondrogenic Potentialmentioning

confidence: 99%

Changes in Chondrogenic Progenitor Populations Associated with Aging and Osteoarthritis

2015

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Chondrogenic progenitor populations, including mesenchymal stem cells, represent promising cell-based transplantation or tissue engineering therapies for the regeneration of damaged cartilage. Osteoarthritis (OA) predominantly affects the elderly and is a leading cause of disability worldwide. Advancing age is a prominent risk factor that is closely associated with the onset and progression of the disease. Understanding the influence that aging and OA have on chondrogenic progenitor cells is important to determine how these processes affect the cellular mechanisms of the cells and their capacity to differentiate into functional chondrocytes for use in therapeutic applications. Here, we review the effect of age-and OA-related changes on the growth kinetics and differentiation potential of chondrogenic progenitor cell populations. Aging differentially influences the proliferative potential of progenitor cells showing reduced growth rates with increased senescence and apoptotic activity over time, while chondrogenesis appears to be independent of donor age. Cartilage tissue affected by OA shows evidence of progenitor populations with some potential for repair, however reports on the proliferative propensity of mesenchymal stem cells and their chondrogenic potential are contradictory. This is likely attributed to the narrow age ranges of samples assessed and deficits in definitively identifying donors with OA versus healthy patients across a wide scope of advancing ages. Further studies that investigate the mechanistic effects of chondrogenic progenitor populations associated with aging and the progression of OA using clearly defined criteria and age-matched control subject groups are crucial to our understanding of the clinical relevance of these cells for use in cartilage repair therapies.

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“…However, clinical applications of these methods would be difficult due to the need for an autologous chondrocyte population. Furthermore, these culture systems are difficult to scale up as larger cultures often result in poor nutrient diffusion leading to necrotic cores [85,86].…”

Section: Chondrogenesis In Ebsmentioning

confidence: 99%

Augmentation of Musculoskeletal Regeneration: Role for Pluripotent Stem Cells

2018

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The rise in the incidence of musculoskeletal diseases is attributed to an increasing ageing population. The debilitating effects of musculoskeletal diseases, coupled with a lack of effective therapies, contribute to huge financial strains on healthcare systems. The focus of regenerative medicine has shifted to pluripotent stem cells (PSCs), namely, human embryonic stem cells and human-induced PSCs, due to the limited success of adult stem cell-based interventions. PSCs constitute a valuable cell source for musculoskeletal regeneration due to their capacity for unlimited self-renewal, ability to differentiate into all cell lineages of the three germ layers and perceived immunoprivileged characteristics. This review summarizes methods for chondrogenic, osteogenic, myogenic and adipogenic differentiation of PSCs and their potential for therapeutic applications.

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Three‐dimensional cartilage tissue engineering using adult stem cells from osteoarthritis patients

Abstract: Three-dimensional hyaline cartilage can be engineered using BMSCs from patients with OA. This method could thus be used for the repair of cartilage lesions.

Cited by 155 publications

References 48 publications

Articular cartilage repair by genetically modified bone marrow aspirate in sheep

Articular cartilage repair by genetically modified bone marrow aspirate in sheep

Changes in Chondrogenic Progenitor Populations Associated with Aging and Osteoarthritis

Augmentation of Musculoskeletal Regeneration: Role for Pluripotent Stem Cells

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