Three-dimensional cell culture based on microfluidic techniques to mimic living tissues

Morimoto, Yuya

doi:10.1039/c2bm00117a

Cited by 48 publications

(28 citation statements)

References 46 publications

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“…However, we did not address the design considerations or cell trapping strategies for microfluidic systems from an engineering point of view. Other works have reviewed the importance of microfluidics in cancer cell culture and drug discovery [74][75][76][77][78][79][80], as well as considering various microfluidic chips enabling 3D cell culture with cancerous or noncancerous cells [9,[81][82][83][84][85][86]. To the best of our knowledge, there is currently no review article available in the literature discussing detailed engineering features and design considerations for such microfluidic systems.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Spheroids-on-a-chip: Recent advances and design considerations in microfluidic platforms for spheroid formation and culture

Moshksayan

Kashaninejad

Warkiani

et al. 2018

Sensors and Actuators B: Chemical

215

175

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Highlights  Cell spheroids are spherical aggregates best mimicking the tissue microenvironment.  Spheroid culture better recapitulates the in-vivo condition in microfluidic chips.  Microfluidics provides rapid spheroid formation with size uniformity and control.  These chips contain microwells, microstructures, droplet generators, etc.  To fabricate such chips, some design considerations must be taken into account.

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Section: Accepted Manuscriptmentioning

confidence: 99%

Spheroids-on-a-chip: Recent advances and design considerations in microfluidic platforms for spheroid formation and culture

Moshksayan

Kashaninejad

Warkiani

et al. 2018

Sensors and Actuators B: Chemical

215

175

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“…Frequently, microwell arrays are cast from low-fouling materials such as agarose, poly(ethylene glycol) or poly(dimethylsiloxane) (PDMS) using moulds manufactured through intensive micromachining, laser ablation or lithography techniques. 14,17,[20][21][22][23][24][25] In addition, microwell array platforms have been studied for the aggregation of dissociated pancreatic mouse islet single cells 26 and insulinproducing cells, 8 although both platforms require specialised and expensive manufacturing techniques, which are not amenable to rapid prototyping of novel microwell designs.…”

Section: Introductionmentioning

confidence: 99%

Rapid fabrication of functionalised poly(dimethylsiloxane) microwells for cell aggregate formation

et al. 2017

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Cell aggregates reproduce many features of the natural architecture of functional tissues, and have therefore become an important in vitro model of tissue function. In this study, we present an efficient and rapid method for the fabrication of site specific functionalised poly(dimethylsiloxane) (PDMS) microwell arrays that promote the formation of insulin-producing beta cell (MIN6) aggregates. Microwells were prepared using an ice templating technique whereby aqueous droplets were frozen on a surface and PDMS was cast on top to form a replica. By employing an aqueous alkali hydroxide solution, we demonstrate exclusive etching and functionalisation of the microwell inner surface, thereby allowing the selective absorption of biological factors within the microwells. Additionally, by manipulating surface wettability of the substrate through plasma polymer coating, the shape and profile of the microwells could be tailored. Microwells coated with antifouling Pluronic 123, bovine serum albumin, collagen type IV or insulin growth factor 2 were employed to investigate the formation and stability of MIN6 aggregates in microwells of different shapes. MIN6 aggregates formed with this technique retained insulin expression. These results demonstrate the potential of this platform for the rapid screening of biological factors influencing the formation and response of insulin-producing cell aggregates without the need for expensive micromachining techniques.

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“…Commonly used methods to produce uniform cellular spheroids include: the hanging drop culture, microfluidic spheroid formation, non‐adherent micropatterned surfaces, and culture in microwell plates . With the proposed platform it is possible to produce arrays of sedimented spheroids and test them in the same platform (see the scheme in Figure a).…”

Section: Resultsmentioning

confidence: 99%

Biomimetic Miniaturized Platform Able to Sustain Arrays of Liquid Droplets for High‐Throughput Combinatorial Tests

Neto

Correia

Custódio

et al. 2014

Adv Funct Materials

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The development of high‐throughput and combinatorial technologies is helping to speed up research that is applicable in many areas of chemistry, engineering, and biology. A new model is proposed for flat devices for the high‐throughput screening of accelerated evaluations of multiplexed processes and reactions taking place in aqueous‐based environments. Superhydrophobic (SH) biomimetic surfaces based on the so‐called lotus effect are produced, onto which arrays of micro‐indentations allow the fixing of liquid droplets, based on the rose‐petal effect. The developed platforms are able to sustain arrays of quasi‐spherical microdroplets, allowing the isolation and confinement of different combinations of substances and living cells. Distinct compartmentalized physical, chemical, and biological processes may take place and be monitored in each droplet. The devices permit the addition/removal of liquid and mechanical stirring by adding magnetic microparticles into each droplet. By facing the chip downward, it is possible to produce arrays of cell spheroids developed by gravity in the suspended droplets, with the potential to be used as microtissues in drug screening tests.

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Three-dimensional cell culture based on microfluidic techniques to mimic living tissues

Cited by 48 publications

References 46 publications

Spheroids-on-a-chip: Recent advances and design considerations in microfluidic platforms for spheroid formation and culture

Spheroids-on-a-chip: Recent advances and design considerations in microfluidic platforms for spheroid formation and culture

Rapid fabrication of functionalised poly(dimethylsiloxane) microwells for cell aggregate formation

Biomimetic Miniaturized Platform Able to Sustain Arrays of Liquid Droplets for High‐Throughput Combinatorial Tests

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