In this work, the inner microstructure of aluminum-silicon casting alloy was experimentally investigated to reveal the spatial morphology and phase distribution. Its industrial use is as a lightweight structural material. As a powerful three-dimensional (3D) imaging tool at the nano-scale, the serial block-face scanning electron microscopy (SBF-SEM) was employed to investigate the specimens. After being 3D reconstructed, the images were visualized to identify preference for several specific intermetallics, by quantitative analysis. It was found that these phases have certain relationships in spatial distribution and differences in spatial shapes. The spatial distribution of cracks was studied to understand the propagation and growth relationship.