Three-dimensional collagen architecture in bovine articular cartilage

Jeffery, A. K.; Blunn, GW; Archer, CW; Bentley, G.

doi:10.1302/0301-620x.73b5.1894669

Cited by 260 publications

(197 citation statements)

References 16 publications

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“…21(d) and (h)]. The orientation of the collagen fibers can be deduced from measurement of the quadrupolar splitting at two orientation of the plug relative to the field according to eqn (3). This indicates the gradual ordering of the collagen fibers during growth, in agreement with biomechanical and EM results obtained for the maturation of rat articular cartilage (69,70).…”

Section: Study Of the Maturation Of Pig Articular Cartilagesupporting

confidence: 76%

“…Articular cartilage can be divided into four zones, which differ in the relative amounts of cartilage constituents and in the collagen fiber orientation: the calcified zone, the radial zone, the intermediate zone and the superficial zone. Collagen fibers rise vertically from the bone and keep the same orientation throughout the calcified and radial zones (2)(3)(4). In the intermediate zone, the fibers bend and then flatten in the superficial zone, where they are aligned parallel to the cartilage surface.…”

Section: Introductionmentioning

confidence: 99%

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Multinuclear NMR and microscopic MRI studies of the articular cartilage nanostructure

Shinar

Navon

2006

NMR in Biomedicine

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Studies of the structure of articular cartilage by a number of NMR spectroscopic and imaging techniques are reviewed. Advantage is taken of the fact that the NMR investigations can be done non-invasively on the intact tissue and do not require sectioning, slicing and decalcification as in the case of electron microscopy. The different contributions to 1 H T 2 relaxation are described and it is pointed out that ignoring the biexponential behavior of the transverse relaxation can lead to serious errors in the proton density measurements and the T 2 characterization of the articular cartilage. A way to slow the transverse relaxation and to minimize its angular dependence by the use of dipolar echo is described. 2 H double quantum filtered spectroscopic MRI is a powerful technique to follow the orientation and density of the collagen fibers in articular cartilage. Using this technique, it was found that attachment of the cartilage to the bone has a stabilizing effect on the collagen matrix and that the hydroxyapatite in the calcified zone resides near the collagen fibers but does not contribute to their order. In response to mechanical pressure, it was shown that the collagen fibers flatten near the surface and become crimped near the bone. A number of NMR techniques have been described for the measurement of 23 Na residual quadrupolar interaction. It was found that this can serve as a very sensitive measure of the depletion of proteoglycans. Finally, a combination of the above techniques was used to study a maturation of articular cartilage in pigs. The increased order and density of the collagen fibers from newborn to adult pigs revealed itself as a shortening of T 2 and significant increase of the residual quadrupolar interaction of both 2 H and 23 Na nuclei.

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Section: Study Of the Maturation Of Pig Articular Cartilagesupporting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Multinuclear NMR and microscopic MRI studies of the articular cartilage nanostructure

Shinar

Navon

2006

NMR in Biomedicine

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“…Over the past two decades, highresolution SEM imaging and cryofracture techniques have suggested that this model be revised. The work of Clark 11 and Jeffrey et al 12 suggest a model shown schematically in Figure 2, in which fibers arising from the subchondral bone form well-defined sheets or lamellae which curve over in the "transitional" layer to then lie parallel to the surface in the most superficial tangential layer. In this model the fibers do not descend back to the bone but terminate in the tangential layer.…”

Section: Cartilage Structurementioning

confidence: 99%

A review of some recent developments in polarization-sensitive optical imaging techniques for the study of articular cartilage

Matcher

2009

Journal of Applied Physics

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This article reviews recent developments in the optical imaging of articular cartilage using polarized-light methods, with an emphasis on tools that could be of use in tissue engineering approaches to treatment. Both second harmonic generation microscopy and polarizationsensitive optical coherence tomography are described and their potential role in the treatment of cartilage disorders such as osteoarthritis is suggested. Key results are reviewed and future developments discussed.2

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“…While the juvenile cartilage shows six-laminar structuring (lower region of the anterior horn), the adult cartilage appears bilaminar under the given measurements conditions bilaminar appearance the cartilage shows a surface zone of high intensity and a broad zone with decreasing intensity from the surface to the bone. These appearances were interpreted as MR visualization of the arcade [or leaf-like (25,26)] model of the hyaline cartilage. Figure 4(a) documents that the MR visualized multilaminarity can be influenced by the adjacent tissue.…”

Section: Age-dependence Of Collageneous Networkmentioning

confidence: 99%

MRI assessment of cartilage ultrastructure

Gründer¹

2006

NMR in Biomedicine

121

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In T 2 -weighted MRI images joint cartilage can appear laminated. The multilaminar appearance is visualized as zones of different intensity. This appearance is based on the dipolar interaction of water molecules within cartilage zones of different collageneous network structures. Therefore, the MR visualization of zones of anisotropic arrangement of the collagen fibers depends upon their orientation to the static magnetic field (magic-angle effect). The aim of this article is to demonstrate the potential of high-resolution MRI for characterizing cartilage network structuring and biomechanical properties. Information equivalent to that from polarization light microscopy can be derived noninvasively. Based on NMR microscopic (mMRI) data, potential new possibilities of MRI for quantitative assessment of collagen structuring and intracartilagenous load distribution are presented. These methods use MR intensity angle dependence and load influence on cartilage visualization. Alternatively to the determination of mechanical parameters from cartilage deformation, it is demonstrated that stress distribution and biomechanical properties can be derived in principle from the local intensity variation of anisotropic fiber orientation zones. The limitations with respect to a clinical application of the proposed methods are discussed.

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Three-dimensional collagen architecture in bovine articular cartilage

Cited by 260 publications

References 16 publications

Multinuclear NMR and microscopic MRI studies of the articular cartilage nanostructure

Multinuclear NMR and microscopic MRI studies of the articular cartilage nanostructure

A review of some recent developments in polarization-sensitive optical imaging techniques for the study of articular cartilage

MRI assessment of cartilage ultrastructure

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