Three-dimensional culture of hepatocytes on porcine liver tissue-derived extracellular matrix

Lang, Ren; Stern, Matthew M.; Smith, Leona; Liu, Yan; Bharadwaj, Shantaram; Liu, Guihua; Baptista, Pedro M.; Bergman, Christopher R.; Söker, Shay; Yoo, James J.; Atala, Anthony; Zhang, Yuanyuan

doi:10.1016/j.biomaterials.2011.06.005

Cited by 125 publications

(110 citation statements)

References 37 publications

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“…The result is an ideal liver specific scaffold for liver engineering that can be seeded with patient specific endothelial cells, hepatocytes, cholangiocytes, or progenitor cells. This will allow for engineering of a transplantable organ specific to the recipient, circumventing the need for immunosuppression [9]. A critical step in tissue engineering a transplantable liver is the creation of a functional vasculature capable of long-term perfusion following anastomosis.…”

Section: Whole Liver Decellularizationmentioning

confidence: 99%

Sustained In Vivo Perfusion of a Re-Endothelialized Tissue Engineered Porcine Liver

Mao¹

2017

Int J Transplant Res Med

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Section: Whole Liver Decellularizationmentioning

confidence: 99%

Sustained In Vivo Perfusion of a Re-Endothelialized Tissue Engineered Porcine Liver

Mao¹

2017

Int J Transplant Res Med

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“…However, our results indicate that primary airway epithelial cell cultures seem to be the most relevant experimental model because they displayed the highest homology in expression pattern and the lowest number of dysregulated genes compared with BM. Improvements of culture conditions, as previously discussed for primary hepatocytes with the change of extracellular matrix or the use of new three-dimensional in vitro models (Kim et al, 2010;Lang et al, 2011), could allow primary airway epithelial cells to present gene expression profiles that accurately reflect those of bronchial tissue, and to be used with full confidence for toxicological and pharmacological studies. It should be noted that the interindividual variability of expression observed for some genes in primary cells may affect the reproducibility of experiments.…”

Section: Number Of Genes Differentially Expressed Between Lung Cell Mmentioning

confidence: 99%

Xenobiotic Metabolism and Disposition in Human Lung Cell Models: Comparison with In Vivo Expression Profiles

et al. 2012

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ABSTRACT:Numerous lung cell lines are currently used as in vitro models for pharmacological and toxicological studies. However, no exhaustive report about the metabolic capacities of these models in comparison with those of lung tissues is available. In the present study, we used a high-throughput quantitative real-time reverse transcription-polymerase chain reaction strategy to characterize the expression profiles of 380 genes encoding proteins involved in the metabolism and disposition of xenobiotics in 10 commonly used lung cell lines (

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“…2 Following the success of perfusion decellularization in the cardiac model, the principle has been applied to other whole organs such as the kidney, [3][4][5][6] lungs, [7][8][9] and liver. 6,[10][11][12][13][14][15][16][17][18] A number of effective strategies for the decellularization of rat livers have been described by Uygun et al, 10 Bao et al 19 and Baptista et al…”

Section: Introductionmentioning

confidence: 99%

Recellularization via the bile duct supports functional allogenic and xenogenic cell growth on a decellularized rat liver scaffold

et al. 2016

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ABSTRACT. Recent years have seen a proliferation of methods leading to successful organ decellularization. In this experiment we examine the feasibility of a decellularized liver construct to support growth of functional multilineage cells. Bio-chamber systems were used to perfuse adult rat livers with 0.1% SDS for 24 hours yielding decellularized liver scaffolds. Initially, we recellularized liver scaffolds using a human tumor cell line (HepG2, introduced via the bile duct). Subsequent studies were performed using either human tumor cells co-cultured with human umbilical vein endothelial cells (HUVECs, introduced via the portal vein) or rat neonatal cell slurry (introduced via the bile duct). Bio-chambers were used to circulate oxygenated growth medium via the portal vein at cholangiocytes was verified by CK-7 positivity. Quantitative albumin measurement from the grafts showed increasing albumin levels after seven days of perfusion. Graft albumin production was higher than that observed in traditional cell culture. This data shows that rat liver scaffolds support human cell ingrowth. The scaffold likewise supported the engraftment and survival of neonatal rat liver cell slurry. Recellularization of liver scaffolds thus presents a promising model for functional liver engineering.

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Three-dimensional culture of hepatocytes on porcine liver tissue-derived extracellular matrix

Cited by 125 publications

References 37 publications

Sustained In Vivo Perfusion of a Re-Endothelialized Tissue Engineered Porcine Liver

Sustained In Vivo Perfusion of a Re-Endothelialized Tissue Engineered Porcine Liver

Xenobiotic Metabolism and Disposition in Human Lung Cell Models: Comparison with In Vivo Expression Profiles

Recellularization via the bile duct supports functional allogenic and xenogenic cell growth on a decellularized rat liver scaffold

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