Three‐dimensional distribution patterns of CD34 antigen on nonactivated cord blood cells

Jung, Georges; Wunder, E.; Dieterlen, Alain; Hénon, Philippe; Jacquey, Serge

doi:10.1002/cyto.a.20490

Cited by 4 publications

(2 citation statements)

References 20 publications

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“…In the earlier study, after enzymatic digestion of de-epithelized stroma of limbal explants, stage specific embryonal antigen 4 (SSEA-4) positive cells were sorted by MACS. The isolated multipotent fibroblast-like cells showed a unique marker profile (CD34 2 , CD45 2 , CD123 2 , Cd133 2 , CD14 2 , CD106 2 , HLA-DR 2 / CD31 1 , SSEA-4 1 , CD73 1 , CD105 1 ), different from that of bone marrow mesenchymal (143,144) or other adult stem cells but similar to that of embryonic stem cells (Oct-4 1 , Sox-2 1 , Tra1-60 1 , Tra1-81 1 ) (141). This marker profile is quite similar to that of very small embryonic-like stem cells of the adult humans (46).…”

Section: Stromal Stem Cellsmentioning

confidence: 97%

Stem cells of the adult cornea: From cytometric markers to therapeutic applications

et al. 2008

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The cornea is a major protective shield of the interior of the eye and represents two thirds of its refractive power. It is made up of three tissue layers that have different developmental origins: the outer, epithelial layer develops from the ectoderm overlying the lens vesicle, whereas the stroma and the endothelium have mesenchymal origin. In the adult organism, the outermost corneal epithelium is the most exposed to environmental damage, and its constant renewal is assured by the epithelial stem cells that reside in the limbus, the circular border of the cornea. Cell turnover in the stromal layer is very slow and the endothelial cells probably do not reproduce in the adult organism. However, recent experimental evidence indicates that stem cells may be found in these layers. Damage to any of the corneal layers leads to loss of transparency and low vision. Corneal limbal stem cell deficiency results in severe ocular surface disease and its treatment by transplantating ex vivo expanded limbal epithelial cells is becoming widely accepted today. Stromal and endothelial stem cells are potential tools of tissue engineering and regenerative therapies of corneal ulcers and endothelial cell loss. In the past few years, intensive research has focused on corneal stem cells aiming to improve the outcomes of the current corneal stem cell transplantation techniques. This review summarizes the current state of knowledge on corneal epithelial, stromal and endothelial stem cells. Special emphasis is placed on the molecular markers that may help to identify these cells, and the recently revealed mechanisms that could maintain their ''stemness'' or drive their differentiation. The techniques for isolating and culturing/ expanding these cells are also described. '

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Section: Stromal Stem Cellsmentioning

confidence: 97%

Stem cells of the adult cornea: From cytometric markers to therapeutic applications

et al. 2008

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“…In Table 4 The canonical discriminant technique has been usefully applied to microscopical images, to distinguish subpopulations of CD34 + cells [20], for automatic classification of colon inflammation diseases [21] or to analyze erythrocyte cell shape [22]. Applied to flow cytometry parameters, as decision support system to diagnose acute leukemia in humans [23] or to evaluate udder health status in cattle [24].…”

Section: Resultsmentioning

confidence: 99%

In‐depth immunophenotyping reveals significant alteration of lymphocytes in buffalo with brucellosis

et al. 2023

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Water buffalo (Bubalus bubalis) has a prominent position in the livestock industry worldwide but still suffers from limited knowledge on the mechanisms regulating the immune against infections, including brucellosis (BRC), one of the most significant neglected zoonotic diseases of livestock. Seventy-three buffalo were recruited for the study. Thirty-five were naturally infected with Brucella spp. The aims of the study were to (i) verify the cross-reactivity of 16 monoclonal antibodies (mAbs) developed against human, bovine, and ovine antigens; (ii) evaluate lymphocyte subset alterations in BRC positive buffalo; (iii) evaluate the use of the canonical discriminant analysis (CDA), with flow cytometric data, to discriminate BRC positive from negative animals. A new set of eight mAbs (anti CD3e, CD16, CD18, CD45R0, CD79a; CD172a) were shown to cross-react with water buffalo orthologous molecules. BRC positive animals presented a significant (p < 0.0001) decrease in the percentage of PBMC (29.5 vs. 40.3), total, T and B lymphocytes (23.0 vs. 35.5, 19.2 vs. 28.9, 2.6 vs. 5.7, respectively). In contrast, they showed an increase in percentage of granulocytes (65.2 vs. 55.1; p < 0.0001) and B lymphocytes CD21 neg (22.9 vs. 16.1; p = 0.0067), a higher T/B lymphocyte ratio (10.3 vs. 6.4; p = 0.0011) and CD3 + /CD21 + (14.7 vs. 8.3; p = 0.0005) ratio. The CDA, applied to 33 different flow cytometric traits, allowed the discrimination of all BRC positive from negative buffalo. Although this is a preliminary study, our results show that flow cytometry can be used in a wide range of applications in livestock diseases, including in support of uncertain BRC diagnoses.

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Polychromatic flow cytometry analysis of CD34+ hematopoietic stem cells in cryopreserved early preterm human cord blood samples

et al. 2010

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During the last decades, extended characterizations were performed of human fullterm cord blood (hTCB) cells, but little information is available on human early preterm cord blood (hEPCB) hematopoietic stem cells (HSCs). In our study, we analyzed by flow cytometry 19 hEPCB and 17 hTCB samples. First, we observed that the percentage of CD34 Pos CD45 Dim cells was higher in hEPCB compared with hTCB and that it decreased during 16th-20th week of pregnancy. Within the CD34 Pos CD45 Dim population, we examined the expression of CD29, CD31, CD38, CD90, CD117, CD133, CD135, CD200, CD243, and CD338. We found that CD135 intensity and CD243 Pos cells percentage were lower in hEPCB compared with hTCB. As to CD38, we observed that hEPCB samples were richer in undifferentiated CD34Pos CD45 Dim CD38 Neg HSCs compared with hTCB counterparts. We also compared the expression of the abovementioned molecules in undifferentiated and committed HSCs residing in hEPCB and hTCB. CD133Pos cells compared with hTCB samples. Finally, analyzing monocytes and lymphocytes within the two samples, we observed that T-cell percentages were higher in hTCB, whereas B-cell percentages were higher in hEPCB. We, therefore, studied the B-cell lineage maturation and found a higher percent of pro-B and pre-B cells in hEPCB compared with hTCB samples. Taken together, these results evidence the hematopoietic peculiarity of hEPCB, potentially useful for highlighting early steps of human hematolymphopoiesis as well as for developing novel strategies of stem cell-based therapy. ' 2010 International Society for Advancement of Cytometry Key termsfetal cord blood; CD34; hematopoietic stem cells; flow cytometry; B-lymphocytes; early preterm cord blood DURING the past decade, human full-term cord blood (HTCB) has been considered to be an attractive hematopoietic stem cells (HSC) source not only for transplantation purposes, but also for basic research investigators studying useful marker to identify immature stages of HSC differentiation (1-3). Compared with mobilized peripheral blood stem cells and marrow HSCs, CB-HSCs are essentially characterized by a series of advantages such as less-stringent HLA-matching requirements between donors and recipients, absence of ethical concerns as well as risks for the mother and the infant, low Epstein Barr virus and cytomegalovirus contaminations, immediate availability of frozen units, and maintenance of ethnic balance by targeted collection programs (1).

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Three‐dimensional distribution patterns of CD34 antigen on nonactivated cord blood cells

Cited by 4 publications

References 20 publications

Stem cells of the adult cornea: From cytometric markers to therapeutic applications

Stem cells of the adult cornea: From cytometric markers to therapeutic applications

In‐depth immunophenotyping reveals significant alteration of lymphocytes in buffalo with brucellosis

Polychromatic flow cytometry analysis of CD34+ hematopoietic stem cells in cryopreserved early preterm human cord blood samples

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