Three-dimensional eco-friendly bacterial nanocellulose (BNC) scaffold for regenerative dentistry: Characterization, cytocompatibility and differentiation potential

Jain, Pooja; Yu-Tong Lin, Ruby; Mishra, Keerti; Handral, Harish; Dubey, Nileshkumar

doi:10.1016/j.dental.2023.11.001

Cited by 4 publications

(1 citation statement)

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“…In addition, their cellular response was verified with viability assays that demonstrate that the stem cells derived from the dental pulp (DPSC) remained viable on Days 1 and 7, having a decrease on Day 14, which coincides with a study previous, where the same cells will be used at different evaluation times (1, 3, 7, 14, and 21 days), decreasing their cell viability from Day 14, the growth peak occurs in the first days, subsequently, it usually decreases because the cells begin to secrete ECM. 52 On the other hand, the SEM micrographs show the efficient cell adhesion on the 3D scaffolds, as well as the cell distribution and morphology, which agrees with that presented by Jain et al 53 Where the cells were aggregated in large groups and the intercellular contact of the cells is observed, the cytoplasmic extensions, confirming the excellent response of cell viability in the 3D printed scaffold (Figures 6 and 7).…”

Section: Discussionsupporting

confidence: 88%

Standardization of 3D printing parameters to control the size and shape of pores in Polylactic acid scaffolds

Pérez‐Sánchez,

Ortiz de la O,

Álvarez‐Pérez

et al. 2024

MedComm – Biomaterials and Applications

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The challenge of three‐dimensional (3D) printing by polymeric extrusion in tissue bioengineering is to control with precision the microarchitecture and porous interconnectivity of scaffolds, as well as search for models that allow and facilitate the development of personalized constructs that meet optimal characteristics for the regeneration of significant bone defects. In this study, anatomically accurate scaffolds were designed and printed to a critical size defect from a microtomography image of the rat calvaria. Different software is used to design a scaffold with exact anatomy. With Ultimaker Cura software, distinct printing parameters were standardized, permitting the printing of different types of pores and graded porosity scaffolds, with exact adaptation to the bone defect, utilizing a commercial 3D printer with a fused deposition modeling technique and compensating for the limitations of the method. The scaffolds were characterized by evaluating their mechanical properties and surface characteristics (pore size and porosity), employing scanning electron microscopy images, verifying that the size and shape of the pores were controlled, and evaluating cell viability and cell distribution on the 3D printed scaffold. Therefore, this work proves that by standardizing the printing parameters, it was possible to print a unique customized scaffold, controlling the shape and size of pores.

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Section: Discussionsupporting

confidence: 88%