An imaging method and optical system, referred to as a line-scanning tomographic optical
microscope (LSTOM) using a combination of line-scanning technique and CT
reconstruction principle, is proposed and studied theoretically and experimentally. In our
implementation a narrow focus line is scanned over the sample and the reflected light is
measured in a confocal arrangement. One such scan is equivalent to a transverse projection
in tomography. Repeating the scanning procedure in several directions, a number
of transverse projections are recorded from which the image can be obtained
using conventional CT reconstruction algorithms. The resolution of the image is
independent of the spatial dimensions and structure of the applied detector; furthermore,
the transfer function of the system is isotropic. The imaging performance of the
implemented confocal LSTOM was compared with a point-scanning confocal microscope,
based on recorded images. These images demonstrate that the resolution of the
confocal LSTOM exceeds (by 15%) the resolution limit of a point-scanning confocal
microscope.