Three-dimensional graphene foams promote osteogenic differentiation of human mesenchymal stem cells

Crowder, Spencer W.; Prasai, Dhiraj; Rath, Rutwik; Balikov, Daniel A.; Bae, Hojae; Bolotin, Kirill I.; Sung, Hak‐Joon

doi:10.1039/c3nr00803g

Cited by 237 publications

(194 citation statements)

References 17 publications

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“…It is suspected that the intrinsic properties of 3D GFs resulted in increased cytoskeletal tension and, thus, guided the cell behavior. 11 However, detailed studies are required to fully understand the mechanism.…”

Section: Mechanical Mechanismmentioning

confidence: 99%

“…The biological activity of graphene-based materials can be associated with their capacities to affect molecular processes and functions. 50 Based on current experiments with regard to cell behaviors in these materials (Table 1), the applications of graphene and its derivatives can be categorized into three types: G sheet, 4,5,51 GO flakes, 6,8,9,11,12,52,53 and rGO flakes, which are directly reduced from GO. 7,10,54 Although GO or rGO films and sheets have been used in different experiments, the main form of a single layer of GO or rGO film or sheet is as a flake or microflake with a diameter less than several micrometers.…”

Section: Graphene and Its Derivativesmentioning

confidence: 99%

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Is Graphene a Promising Nano-Material for Promoting Surface Modification of Implants or Scaffold Materials in Bone Tissue Engineering?

Liu

Chen

et al. 2014

Tissue Engineering Part B: Reviews

110

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Bone tissue engineering promises to restore bone defects that are caused by severe trauma, congenital malformations, tumors, and nonunion fractures. How to effectively promote the proliferation and osteogenic differentiation of mesenchymal stem cells (MSCs) or seed cells has become a hot topic in this field. Many researchers are studying the ways of conferring a pro-osteodifferentiation or osteoinductive capability on implants or scaffold materials, where osteogenesis of seed cells is promoted. Graphene (G) provides a new kind of coating material that may confer the pro-osteodifferentiation capability on implants and scaffold materials by surface modification. Here, we review recent studies on the effects of graphene on surface modifications of implants or scaffold materials. The ability of graphene to improve the mechanical and biological properties of implants or scaffold materials, such as nitinol and carbon nanotubes, and its ability to promote the adhesion, proliferation, and osteogenic differentiation of MSCs or osteoblasts have been demonstrated in several studies. Most previous studies were performed in vitro, but further studies will explore the mechanisms of graphene's effects on bone regeneration, its in vivo biocompatibility, its ability to promote osteodifferentiation, and its potential applications in bone tissue engineering.

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Section: Mechanical Mechanismmentioning

confidence: 99%

Section: Graphene and Its Derivativesmentioning

confidence: 99%

Is Graphene a Promising Nano-Material for Promoting Surface Modification of Implants or Scaffold Materials in Bone Tissue Engineering?

Liu

Chen

et al. 2014

Tissue Engineering Part B: Reviews

110

View full text Add to dashboard Cite

show abstract

“…[ 22,23 ] Because of these properties and its inherent structure, which can better mimic the physiological environment conducive for cell growth than gels or polymers, 3D-C has great potential in cell culture. This potential was evident in studies investigating its differentiation effects with neural stem cells (NSCs) [ 20 ] and human mesenchymal stem cells, [ 24 ] which both demonstrated the possibility of differentiation through 3D-C. Moreover, 3D-C's scaffold architecture also provides support for NSC growth and proliferation.…”

mentioning

confidence: 96%

Three‐Dimensional Graphene: A Biocompatible and Biodegradable Scaffold with Enhanced Oxygenation

Loeblein

Perry

Tsang

et al. 2016

Adv Healthcare Materials

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Owing to its high porosity, specific surface area and three-dimensional structure, three-dimensional graphene (3D-C) is a promising scaffold material for tissue engineering, regenerative medicine as well as providing a more biologically relevant platform for living organisms in vivo studies. Recently, its differentiation effects on cells growth and anti-inflammation properties have also been demonstrated. Here, we report a complete study of 3D-C as a fully adequate scaffold for tissue engineering and systematically analyze its biocompatibility and biodegradation mechanism. The metabolic activities of liver cells (HepG2 hepatocarcinoma cells) on 3D-C are studied and our findings show that cell growth on 3D-C has high cell viability (> 90%), low lactate production (reduced by 300%) and its porous structure also provides an excellent oxygenation platform. 3D-C is also biodegradable via a 2-step oxidative biodegradation process by first, disruption of domains and lift off of smaller graphitic particles from the surface of the 3D-C and subsequently, the decomposition of these graphitic flakes. In addition, the speed of the biodegradation can be tuned with pretreatment of O2 plasma.

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“…Graphene derivatives show antibacterial impact because of layer disruption [90]. Since it is cost-effective, it opens a new door in clinical and ecological applications of GO [29]. If we compare with GO, Gt, rGO and GtO graphene subordinates, it has been found out that the antibacterial property diminishes in them as we move towards GtO.…”

Section: Substrates For Antibacterial Effectsmentioning

confidence: 99%

“…Graphene-based materials are used in the field of bone repair or organ regeneration. Both GO and rGO are utilized in osteogenic stem cells to study [28][29][30] chondrogenesis [31], adipogenesis [32], epithelial genesis [32], myogenesis, cardiomyogenesis [33,34] and neurogenesis [35].…”

Section: Introductionmentioning

confidence: 99%

Graphene and graphene oxide as nanomaterials for medicine and biology application

et al. 2018

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Graphene-and graphene oxide-based nanomaterials have gained broad interests in research because of their unique physiochemical properties. The 2D allotropic structure allows it to be used in various biological fields. The biomedical applications of graphene and its composite include its use in gene and small molecular drug delivery. It is further used for biofunctionalization of protein, in anticancer therapy, as an antimicrobial agent for bone and teeth implantation. The biocompatibility of the newly synthesized nanomaterials allows its substantial use in medicine and biology. The current review summarizes the chemical structure and biological application of graphene in various fields.

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Three-dimensional graphene foams promote osteogenic differentiation of human mesenchymal stem cells

Cited by 237 publications

References 17 publications

Is Graphene a Promising Nano-Material for Promoting Surface Modification of Implants or Scaffold Materials in Bone Tissue Engineering?

Is Graphene a Promising Nano-Material for Promoting Surface Modification of Implants or Scaffold Materials in Bone Tissue Engineering?

Three‐Dimensional Graphene: A Biocompatible and Biodegradable Scaffold with Enhanced Oxygenation

Graphene and graphene oxide as nanomaterials for medicine and biology application

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