Three-dimensional human skin model infected with Staphylococcus aureus as a tool for evaluation of bioactivity and biocompatibility of antiseptics

Reddersen, Kirsten; Wiegand, Cornelia; Elsner, Peter; Hipler, Uta‐Christina

doi:10.1016/j.ijantimicag.2019.06.022

Cited by 17 publications

(24 citation statements)

References 35 publications

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“…After submerged cultivation, the full skin models were cultivated at the air‐medium border (airlift cultivation) for a further 12 days. For this purpose, the full skin models were transferred to ThinCert 12‐well plates (Greiner Bio‐One) and supplemented with Green's medium (1:1 DMEM + DMEM HAMS‐F12 (Gibco, Thermo Fisher Scientific), as previously reported (Reddersen et al, 2019 ; Wiegand et al, 2016 ). The medium was changed every 2–3 days.…”

Section: Methodsmentioning

confidence: 99%

“…The images were processed and evaluated using the ImageJ program (National Institutes of Health, 2004). Cell viability was determined based on the luminometric ATP measurement using the ATPLiteTM‐M assay (PerkinElmer) as previously reported (Reddersen et al, 2019 ; Wiegand et al, 2016 ). In brief, 500 µl of the cell lysis solution was added to each full skin model.…”

Section: Methodsmentioning

confidence: 99%

“…In vivo conditions are increasingly being mimicked using three‐dimensional (3D) cell cultures. Such full skin models consist of a collagen matrix populated with primary human fibroblasts as dermis and a fully differentiated epidermis made of primary human keratinocytes (Reddersen et al, 2019 ; Wiegand et al, 2016 ). For the investigations in this study, the model was artificially infected with spores to enable the analysis of the sporicidal influence of the substances to be examined under relevant in vivo‐like conditions.…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of antifungal agents against fungal spores: An in vitro study using microplate laser nephelometry and an artificially infected 3D skin model

et al. 2022

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Dermal fungal infections seem to have increased over recent years. There is further a shift from anthropophilic dermatophytes to a growing prevalence of zoophilic species and the emergence of resistant strains. New antifungals are needed to combat these fungi and their resting spores. This study aimed to investigate the sporicidal effects of sertaconazole nitrate using microplate laser nephelometry against the microconidia of Trichophyton , chlamydospores of Epidermophyton , blastospores of Candida , and conidia of the mold Scopulariopsis brevicaulis . The results obtained were compared with those from ciclopirox olamine and terbinafine. The sporicidal activity was further determined using infected three‐dimensional full skin models to determine the antifungal effects in the presence of human cells. Sertaconazole nitrate inhibited the growth of dermatophytes, molds, and yeasts. Ciclopirox olamine also had good antifungal activity, although higher concentrations were needed compared to sertaconazole nitrate. Terbinafine was highly effective against most dermatophytes, but higher concentrations were required to kill the resistant strain Trichophyton indotineae . Sertaconazole nitrate, ciclopirox olamine, and terbinafine had no negative effects on full skin models. Sertaconazole nitrate reduced the growth of fungal and yeast spores over 72 h. Ciclopirox olamine and terbinafine also inhibited the growth of dermatophytes and molds but had significantly lower effects on the yeast. Sertaconazole nitrate might have advantages over the commonly used antifungals ciclopirox olamine and terbinafine in combating resting spores, which persist in the tissues, and thus in the therapy of recurring dermatomycoses.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Efficacy of antifungal agents against fungal spores: An in vitro study using microplate laser nephelometry and an artificially infected 3D skin model

et al. 2022

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“…Human skin equivalents have been successfully used as model systems in infection research, e.g. with Candida albicans (Kuhbacher et al, 2017), Staphylococcus aureus (Reddersen et al, 2019), and helminths (Jannasch et al, 2015). However, a major problem with collagen-based skin equivalents is their susceptibility to fibroblast-mediated contraction during culture.…”

Section: Introductionmentioning

confidence: 99%

Vector-borne Trypanosoma brucei parasites develop in artificial human skin and persist as skin tissue forms

Reuter

Imdahl

Hauf

et al. 2021

Preprint

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Transmission of Trypanosoma brucei by tsetse flies involves the deposition of the cell cycle- arrested metacyclic life cycle stage into mammalian skin at the site of the fly's bite. In the skin, the metacyclic parasites reactivate and differentiate into proliferative trypanosomes before colonizing the host's blood and tissues. We generated an advanced human skin equivalent and used tsetse flies to naturally infect the artificial skin with trypanosomes. We detailed the chronological order of the parasites' development in the skin, and found a rapid activation of metacyclic trypanosomes and differentiation to proliferative parasites. Single-cell parasite transcriptomics were used to document the biological events during differentiation and host invasion at five different timepoints. After the establishment of a proliferative trypanosome population in the skin, the parasites entered a reversible quiescent state characterized by slow replication and a strongly reduced metabolism. We termed these quiescent trypanosomes skin tissue forms (STF), a parasite population that may play an important role in maintaining the infection over long time periods and in asymptomatic infected individuals.

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“…6 PHMB was used for several years as an antiseptic solution in medical applications without evidence of bacterial resistance when used topically on skin, eyes, and wounds. [7][8] It has also shown little toxicity, has a good safety record, good efficacy, low cost, and tolerability. 10,11 In dentistry, the inhibitory action of PHMB as an antiseptic in the formation of oral biofilm has been studied, showing a reduction in the amount of bacteria.…”

Section: Introductionmentioning

confidence: 99%

Perception and acceptance of the use of 0.2% polyhexanide versus 0.12% chlorhexidine digluconate in patients at a risk of developing oral mucositis.

Santaella¹,

Gonçales²,

Martins³

et al. 2020

J Oral Res

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Objective: To evaluate the perception and acceptance of using polyhexanide (PHMB) and chlorhexidine digluconate (CLX) in individuals at a risk of developing oral mucositis induced by chemoradiotherapy. Materials and Methods: This is a randomised comparative study. Participants were randomised into two groups: Group 1 (PHMB 0.2%) and Group 2 (CLX 0.12%), these groups performed a mouth rinse with the respective solutions for 1 minute every 12 hours during an antineoplastic treatment cycle. The participants were evaluated at three different times: before (t0), during (t1) and after a cycle of antineoplastic treatment (t2). Severity of oral mucositis (OM), mouth pain, quality of life (OHIP-14), oral hygiene index and assessment of the acceptance of the substances in the mouth were assessed. Results: There were 23 individuals, 12 in Group 1 (G1) and 11 in Group 2 (G2). Both groups presented with OM in all three evaluations. Reported mouth pain was lower in G1 than in G2. The PHMB had a better acceptance (p=0.012) than the CLX for the time of mouth rinse at t0. There was a lower impact in the quality of life from oral health in the physical pain aspect (p=0.019) and in social incapacity (p=0.037) in G1 than in G2. Conclusions: PHMB has the same acceptance compared to CLX and is a good option for antiseptic mouth rinse with less adverse effects

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Three-dimensional human skin model infected with Staphylococcus aureus as a tool for evaluation of bioactivity and biocompatibility of antiseptics

Cited by 17 publications

References 35 publications

Efficacy of antifungal agents against fungal spores: An in vitro study using microplate laser nephelometry and an artificially infected 3D skin model

Efficacy of antifungal agents against fungal spores: An in vitro study using microplate laser nephelometry and an artificially infected 3D skin model

Vector-borne Trypanosoma brucei parasites develop in artificial human skin and persist as skin tissue forms

Perception and acceptance of the use of 0.2% polyhexanide versus 0.12% chlorhexidine digluconate in patients at a risk of developing oral mucositis.

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