2022
DOI: 10.1111/pin.13222
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Three‐dimensional imaging of intramural perineural invasion in colorectal cancer: Three‐dimensional reconstruction approach with multiple immunohistochemically stained sections

Abstract: Perineural invasion (PNI) at Auerbach's plexus in colorectal cancer (CRC), known as intramural PNI, is associated with adverse prognostic outcomes. This study aimed to characterize the three‐dimensional (3D) architecture of CRC with intramural PNI and to evaluate the morphological features of tumor invasion around nerve tissue. Serial tissue sections from two cases of CRC were stained with cytokeratin AE1/AE3 and an anti‐S‐100 protein antibody. 3D models were reconstructed by scanning the virtual slides. In on… Show more

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Cited by 4 publications
(3 citation statements)
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“…Second, 3D reconstruction of blood vessels can help clinicians predict the type of blood vessels before surgery, find blood vessels during surgery, and correctly anatomize blood vessels. This is especially helpful for people who are very overweight, have experienced major changes in their anatomy, or have severe abdominal adhesions[ 35 ]. Third, 3D vascular reconstruction technology may help avoid some intraoperative complications, such as visceral damage, vascular damage, and bleeding, which are some of the main reasons for prolonged surgical time.…”
Section: Discussionmentioning
confidence: 99%
“…Second, 3D reconstruction of blood vessels can help clinicians predict the type of blood vessels before surgery, find blood vessels during surgery, and correctly anatomize blood vessels. This is especially helpful for people who are very overweight, have experienced major changes in their anatomy, or have severe abdominal adhesions[ 35 ]. Third, 3D vascular reconstruction technology may help avoid some intraoperative complications, such as visceral damage, vascular damage, and bleeding, which are some of the main reasons for prolonged surgical time.…”
Section: Discussionmentioning
confidence: 99%
“…Tissue clearing followed by three-dimensional (3D) imaging is a technique that enables the simultaneous analysis of multiple cells within an organ. Masaki Mori et al were the first to characterize the 3D structure of tumor invasion around nerve tissue in CRC using this method [ 178 ]. By staining CRC tissue with cytokeratin AE1/AE3 and anti-S100 protein antibody, virtual slides were scanned to reconstruct the tissue, effectively demonstrating the morphological features of intramural PNI in CRC [ 178 ].…”
Section: Models To Study Pnimentioning
confidence: 99%
“…However, the resolution of 3D analysis was low, and the observation was limited to a small area around the nerve fibers [ 11 ]. Miyashita et al examined the 3D structure of PNI in Auerbach’s plexus in a cohort of patients with colorectal cancer using the reconstruction of serial sections and reported the presence of two PNI types, PNI with preserved nerve structure and PNI with damaged the nerve structure [ 12 ]. However, the structural relationship between nerve fibers and cancer across the full thickness of the bile duct wall using 3D imaging methods has not yet been examined.…”
Section: Introductionmentioning
confidence: 99%