Three-dimensional liquid chromatography with parallel second dimensions and quadruple parallel mass spectrometry for adult/infant formula analysis

Fast chromatography of pulse triacylglycerols

“…Samples were ground from raw pulses and stored at À80 C. Market class pulses were obtained as ground samples from Prof. John Finley from Louisiana State University, via Dr. Dave Luthria and Dr. Raghavendhar Kotha, who previously analyzed these samples and reported the oligosaccharide contents (Kotha et al, 2020). Pulses were extracted using the method of Folch et al (1957) with minor modifications (Byrdwell et al, 2022). Vitamin D2 was added prior to extraction, as an extraction internal standard (EIS) for fat-soluble vitamins (FSVs), and menaquinone, vitamin K2, was added prior to making sample to final volume in solution, as an analytical internal standard (AIS) for FSVs, both at a concentrations of 2.0 μg/ml.…”

Less Configuration and More Dimensionality: Preparative Heart-Cut Multidimensional Liquid Chromatography Based on Trapping Arrays

“…Then, by switching V1, the 3 D separation was initiated (Figure 3C). The trapped sample in trapping array 1 (indicating the first trap column at the current V3 status) was introduced to the 3 Using a 30 mm I.D. column in 1 D and loading 3 g of sample, the dilute ratio was 1:1.5 due to the limited flow rate of the trap columns, and the trapped fractions from 1 D to 2 D were decreased from 18 to 9; these factors induced the overload of trap columns.…”

“…2 As commercially available systems have become routinely available, two-dimensional liquid/gas chromatography (2D-LC, 2D-GC) has become increasingly popular among various multidimensional separation methods. 3 Improvements in the combination of multidimensional techniques with spectroscopy/spectrometry have been informative for chemical profiling, and multicomponent detection; however, these advances have not provided any benefits to purification laboratories historically. Traditional one-dimensional LC techniques remain the dominating method to tediously isolating compounds with a certain purity.…”

“…The identification and characterization of target components in complex mixtures has been significantly impacted by the extraordinary resolving power of multidimensional separation combined with trapping-enrichment and online deuterated solvent exchange features, which is important at nearly every stage of chemical and biological research (e.g., natural product, drug discovery and development, and -omics research) . As commercially available systems have become routinely available, two-dimensional liquid/gas chromatography (2D-LC, 2D-GC) has become increasingly popular among various multidimensional separation methods . Improvements in the combination of multidimensional techniques with spectroscopy/spectrometry have been informative for chemical profiling, and multicomponent detection; however, these advances have not provided any benefits to purification laboratories historically.…”

“…Although preparative 2D-LC methods have been established and are quite powerful, exhaustive separations of very complex samples are still challenging. , This has motivated researchers to investigate the potential benefits of adding more LC columns to a 2D-LC system . Offline 3D-LC in the preparative scale and several online 3D-LCs in the analytical scale ,, have been developed. Recently, a trapping-enrichment mD-LC framework with online deuterated solvent exchange for streamlined structure elucidation at the microgram scale was introduced.…”