Three‐dimensional magnetic resonance microscopy of pulmonary solitary tumors in transgenic mice

Kubo, Shigeto; Kobayashi, Susumu; Kocher, Olivier; Halmos, Balázs; Tenen, Daniel G.; Takahashi, Masaya

doi:10.1002/mrm.21006

Cited by 13 publications

(9 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The smallest lesion detected in this study was 0.5 mm in diameter, as in higher-field MRI [10],[17]. Kubo et al assessed MR imaging of lung tumors using a 4.7 Tesla superconducting MR system and found that abnormalities were detected with a more than 96% histopathological correlation [20]. In their research, other lung abnormalities, such as atelectasis, pleural thickening and etc., were also evaluated besides lung tumor.…”

Section: Discussionsupporting

confidence: 48%

“…In previous research assessing lung tumors, most researchers applied respiratory gating [7],[18],[19],[22],[24], some used both respiratory and cardiac gating [8],[9],[17],[20],[21],[23], and acceptable image quality was generally achieved. In this study, neither respiratory nor cardiac gating was applied, however, minor or modest artifacts, acceptable for routine, did not impair image evaluation and MRI is capable of showing submillimeter lesions.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Detection of Lung Tumors in Mice Using a 1-Tesla Compact Magnetic Resonance Imaging System

et al. 2014

View full text Add to dashboard Cite

Due to their small size, lung tumors in rodents are typically investigated using high-field magnetic resonance (MR) systems (4.7 T or higher) to achieve higher signal-to-noise ratios, although low-field MR systems are less sensitive to susceptibility artifacts caused by air in the lung. We investigated the feasibility of detecting lung tumors in living, freely breathing mice with a 1-T compact permanent magnet MR system. In total, 4 mice were used, and MR images of mouse lungs were acquired using a T1-weighted three-dimensional fast low-angle shot sequence without cardiac or respiratory gating. The delineation and size of lung tumors were assessed and compared with histopathological findings. Submillimeter lesions were demonstrated as hyperintense, relative to the surrounding lung parenchyma, and were delineated clearly. Among the 13 lesions validated in histopathological sections, 11 were detected in MR images; the MR detection rate was thus 84.6%. A strong correlation was obtained in size measurements between MR images and histological sections. Thus, a dedicated low-field MR system can be used to detect lung tumors in living mice noninvasively without gating.

show abstract

Section: Discussionsupporting

confidence: 48%

Section: Discussionmentioning

confidence: 99%

Detection of Lung Tumors in Mice Using a 1-Tesla Compact Magnetic Resonance Imaging System

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Unlike brain imaging, in-vivo MRI of the lung is challenging because of the inherent difficulties associated with properties of the organ, including respiratory and cardiac motion artifacts, severe magnetic field susceptibility arising from large air-tissue interfaces [20], [21]. In particular, the pronounced susceptibility effects in the lung may dynamically alter the magnetic field homogeneity during respiratory cycle and thus may cause shifts in the resonance frequencies of the different proton pools in the tissue.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Lung Cancer by Amide Proton Transfer (APT) Imaging: An In-Vivo Study in an Orthotopic Mouse Model

et al. 2013

Self Cite

View full text Add to dashboard Cite

Amide proton transfer (APT) imaging is one of the chemical exchange saturation transfer (CEST) imaging methods which images the exchange between protons of free tissue water and the amide groups (−NH) of endogenous mobile proteins and peptides. Previous work suggested the ability of APT imaging for characterization of the tumoral grade in the brain tumor. In this study, we tested the feasibility of in-vivo APT imaging of lung tumor and investigated whether the method could differentiate the tumoral types on orthotopic tumor xenografts from two malignant lung cancer cell lines. The results revealed that APT imaging is feasible to quantify lung tumors in the moving lung. The measured APT effect was higher in the tumor which exhibited more active proliferation than the other. The present study demonstrates that APT imaging has the potential to provide a characterization test to differentiate types or grade of lung cancer noninvasively, which may eventually reduce the need invasive needle biopsy or resection for lung cancer.

show abstract

“…MRI has been used to detect primary tumor as well as metastases to the rodent lung 54–56 and provides the advantage of assessing treatment effect in the same animal without the use of other imaging modalities that utilize ionizing radiation. Although imaging the murine lung can prove challenging due to organ motion 57, it is feasible, especially with the benefit of respiratory and cardiac gating 58–60. Our initial studies establish that tumor volume measured by MRI is highly correlated with the mass of tumor collected and weighed at necropsy, and thereby provides a good representation of total tumor burden.…”

Section: Discussionmentioning

confidence: 79%

Photodynamic therapy of disseminated non-small cell lung carcinoma in a murine model

et al. 2011

View full text Add to dashboard Cite

Background and Objective Photodynamic therapy (PDT) of thoracic malignancies involving the pleural surfaces is an active area of clinical investigation. The present report aims to characterize a model for PDT of disseminated non-small cell lung carcinoma grown orthotopically in nude mice, and to evaluate PDT effect on tumor and normal tissues. Study Design H460 human non-small cell lung carcinoma (NSCLC) cells were injected percutaneously into the thoracic cavity of nude mice. HPPH-PDT (1 mg/kg, 24 h) was performed via the interstitial delivery (150 mW/cm) of 661 nm light to the thoracic cavity at fluences of 25-200 J/cm. Results H460 tumors exhibited exponential growth within the thoracic cavity consisting of diffuse, gross nodular disease within 9 days after intrathoracic injection. Tumor volume, measured by magnetic resonance imaging (MRI), was highly correlated with the aggregate tumor mass extracted from the corresponding animal. Intrathoracic PDT at fluences of ≥ 50 J/cm produced significant decreases in tumor burden as compared to untreated controls, however mortality increased with rising fluence. Accordingly, 50 J/cm was selected for MRI studies to measure intra-animal PDT effects. Tumor distribution favored the ventral (vs. dorsal), caudal (vs. cranial), and right (vs. left) sides of the thoracic cavity by MRI; PDT did not change this spatial pattern despite an overall effect on tumor burden. Histopathology revealed edema and fibrin deposition within the pulmonary interstitium and alveoli of the PDT-treated thoracic cavity, as well as occasional evidence of vascular disruption. Prominent neutrophil infiltration with a concomitant decline in the lymphocyte compartment was also noted in the lung parenchyma within 24 hours after PDT. Conclusion HPPH-PDT of an orthotopic model of disseminated NSCLC is both feasible and effective using intracavitary light delivery. We establish this animal model, together with the treatment and monitoring approaches, as novel and valuable methods for the pre-clinical investigation of intrathoracic PDT of disseminated pleural malignancies.

show abstract

Three‐dimensional magnetic resonance microscopy of pulmonary solitary tumors in transgenic mice

Cited by 13 publications

References 19 publications

Detection of Lung Tumors in Mice Using a 1-Tesla Compact Magnetic Resonance Imaging System

Detection of Lung Tumors in Mice Using a 1-Tesla Compact Magnetic Resonance Imaging System

Characterization of Lung Cancer by Amide Proton Transfer (APT) Imaging: An In-Vivo Study in an Orthotopic Mouse Model

Photodynamic therapy of disseminated non-small cell lung carcinoma in a murine model

Contact Info

Product

Resources

About